Culture systems for transformations of hydatid cysts from protoscoleces
in vitro - Journal of Third Military Medical University Vol. 41, Issue 5, Pages: 415-423(2019)
- 作者机构：
400016 重庆，重庆医科大学基础医学院病原生物学教研室，分子医学与肿瘤研究中心
- 作者简介：
- 基金信息：the General Program of National Natural Science Foundation of China(81672045)
DOI：10.16016/j.1000-5404.201808123
CLC： Q95-331;Q959.175;R383.33Received：18 August 2018，
Revised：2018-11-05，
Published：15 March 2019
- 稿件说明：
移动端阅览
Hongye WU, Kai LI, Fen WANG, et al. Culture systems for transformations of hydatid cysts from protoscoleces
in vitro [J]. Journal of Third Military Medical University, 2019, 41(5): 415-423.Hongye WU, Kai LI, Fen WANG, et al. Culture systems for transformations of hydatid cysts from protoscoleces
in vitro [J]. Journal of Third Military Medical University, 2019, 41(5): 415-423. DOI： 10.16016/j.1000-5404.201808123.
摘要
目的
2
建立适合细粒棘球绦虫原头蚴在体外形成棘球蚴的培养体系。
方法
2
以RPMI1640或DMEM培养基为基质，胎牛血清或绵羊血清为营养成分，人肝细胞株L02为饲养细胞设计配伍2大类、6个组培养体系；培养细粒棘球绦虫原头蚴45 d，根据各组原头蚴的头节外翻率、成囊率和囊泡大小情况，寻找适合原头蚴形成棘球蚴的体外培养体系最佳配伍；在最佳配伍的培养体系继续培养棘球蚴囊泡至180 d。
结果
2
在起初的45 d期间，DMEM体系的E组(DMEM+胎牛血清+人肝细胞株L02)培养的棘球蚴生长最快，20 d起原头蚴成囊率高于其他各组(
P
<
0.05)，25 d起棘球蚴囊泡大于其他各组(
P
<
0.05)，45 d时原头蚴成囊率达到100%；在其后的46~180 d内，棘球蚴囊泡持续增大，至80 d时肉眼可见透明囊泡，至180 d时最大囊泡直径达2.2 mm。
结论
2
添加胎牛血清及人肝细胞株L02的DMEM培养基有利于体外培养的原头蚴发育为棘球蚴囊泡。
Abstract
Objective
2
To search for a suitable culture system for transforming p
rotoscoleces of
Echinococcus granulosus
into hydatid cysts
in vitro
.
Methods
2
Based on the basic cell culture media RPMI1640 or DMEM
and fetal calf serum or sheep serum as nutrients
and human liver cell line L02 as server cells
culture systems for transformations of protoscoleces into hydatid cysts
in vitro
were established based on block designs. The culture systems were divided into 2 main groups
and 6 subgroups according the different ingredients of culture media
sera
and sever cells. The protoscoleces were cultured in 6 subgroups for 45 d
and then the most suitable medium for the transformations was screened according to the ratios of evaginated or encysted protoscoleces
and the sizes of protoscoleces. In the selected best culture system
the encysted protoscoleces were kept cultivating up to 180 d for further observation.
Results
2
Within the first period of 45 d
it demonstrated that the culture system of DMEM medium+fetal calf serum+L02 cells was the best culture system for transformations of protoscoleces into hydatid cysts
in vitro
because of the fastest growth speed of protoscoleces
the maximal ratio of encystation of protoscoleces since 20 d (
P
<
0.05)
the biggest size of hydatid cysts since 25 d (
P
<
0.05)
and 100% percentage of encystation of protoscoleces at 45 d. In the following culture days (46 to 180 d)
the hydatid cysts still grew up
the transparent hydatid cysts were visible to the naked eyes at 80 d
and the biggest cyst size reached to 2.2 mm at 180 d.
Conclusion
2
The culture medium of DMEM
added with fetal calf serum and L02 cells
is a suitable culture system for transformations of protoscoleces into hydatid cysts
in vitro
.
关键词
Keywords
references
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