Bone marrow transplantation promotes tumor growth and lung metastasis in C57BL/6 subcutaneous tumor-bearing mice

Xiao HAN; Dengqun LIU; Dan LUO; Guoxiang LIU

doi:10.16016/j.1000-5404.201808111

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Bone marrow transplantation promotes tumor growth and lung metastasis in C57BL/6 subcutaneous tumor-bearing mice

更新时间：2026-01-09

- Bone marrow transplantation promotes tumor growth and lung metastasis in C57BL/6 subcutaneous tumor-bearing mice
- Journal of Third Military Medical University Vol. 40, Issue 21, Pages: 1932-1935(2018)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81472697);the General Program of National Natural Science Foundation of China(81673089)
- DOI：10.16016/j.1000-5404.201808111
  CLC：
- Received：12 August 2018，
  
  Published：15 November 2018
- 稿件说明：
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Xiao HAN, Dengqun LIU, Dan LUO, et al. Bone marrow transplantation promotes tumor growth and lung metastasis in C57BL/6 subcutaneous tumor-bearing mice[J]. Journal of Third Military Medical University, 2018, 40(21): 1932-1935.
DOI：

Xiao HAN, Dengqun LIU, Dan LUO, et al. Bone marrow transplantation promotes tumor growth and lung metastasis in C57BL/6 subcutaneous tumor-bearing mice[J]. Journal of Third Military Medical University, 2018, 40(21): 1932-1935. DOI： 10.16016/j.1000-5404.201808111.

摘要

Abstract

Objective

To investigate the effect of bone marrow transplantation on the tumorigenesis and metastasis of subcutaneously inoculated Lewis lung cancer(LLC) cells in C57BL/6(C57) mice.

Methods

After irradiated with 8 Gy carbon-ion beam to destroy the bone marrow hematopoietic system

the mice were transplanted with the bone marrow cells collected from normal C57 mice

via

tail vein injection to establish a mouse model of bone marrow transplantation(BMT). Then

both BMT mice and normal C57 mice were subcutaneously injected with LLC cells on the back to build a model of lung tumor-bearing mice

Then the tumor size

tumor weight and lung metastasis were recorded to evaluate the effect of BMT.

Results

The tumor masses could be touched on day 5 after subcutaneous tumor implantation(day 0)

which was in 2 d earlier than in the C57 mice. At the time of sacrifice day(day 19)

the BMT mice had signif

icantly larger tumor size and heavier weight compared with normal C57 mice(

0.01). Moreover

obvious lung metastasis foci were observed in BMT mice but not in normal C57 mice.

Conclusion

BMT C57 mice are more suitable for subcutaneous tumor modeling than normal C57 ones

with larger tumor size and easier for metastasis.

关键词

Keywords

references

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