Long non-coding RNA LSINCT5 promotes proliferation and metastasis of papillary thyroid cancer by binding to miR-29c

Jianren KUANG; Ping WEI

doi:10.16016/j.1000-5404.201807173

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Long non-coding RNA LSINCT5 promotes proliferation and metastasis of papillary thyroid cancer by binding to miR-29c

Basic Medicine | 更新时间：2026-01-09

- Long non-coding RNA LSINCT5 promotes proliferation and metastasis of papillary thyroid cancer by binding to miR-29c
- Journal of Third Military Medical University Vol. 40, Issue 20, Pages: 1851-1857(2018)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院内分泌科
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201807173
  CLC： R394.13;R730.23;R736.1
- Received：11 July 2018，
  
  Revised：2018-08-30，
  
  Published：30 October 2018
- 稿件说明：
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Jianren KUANG, Ping WEI. Long non-coding RNA LSINCT5 promotes proliferation and metastasis of papillary thyroid cancer by binding to miR-29c[J]. Journal of Third Military Medical University, 2018, 40(20): 1851-1857.
DOI：

Jianren KUANG, Ping WEI. Long non-coding RNA LSINCT5 promotes proliferation and metastasis of papillary thyroid cancer by binding to miR-29c[J]. Journal of Third Military Medical University, 2018, 40(20): 1851-1857. DOI： 10.16016/j.1000-5404.201807173.

摘要

目的

探讨lncRNA LSINCT5靶向结合miR-29c促乳头状甲状腺癌(papillary thyroid cancer，PTC)增殖和迁移的作用及机制。

方法

高通量转录芯片筛选PTC癌组织和癌旁组织差异表达lncRNAs。实时荧光定量PCR(qRT-PCR)检测32例来源于本院的PTC配对癌组织和癌旁组织样本(其中男性18例，女性14例，平均年龄57岁)和PTC细胞系中LSINCT5的表达水平。PTC细胞株TPC-1和KAT-5中转染siNC、siLSINCT5-1、siLSINCT5-2后，CCK-8和Transwell迁移实验测定转染各组的细胞增殖和转移水平。采用双荧光素酶报告基因实验验证LSINCT5与miR-29c的靶向结合。TPC-1细胞中转染Control(空质粒组)、LSINCT5、LSINCT5-Mut以及共转染LSINCT5和miR-29c后，qRT-PCR和Western blot检测ITGB1和E-cadherin表达水平。

结果

与配对的癌旁组织相比，LSINCT5在癌组织中表达显著升高(

0.05)。LSINCT5在PTC细胞系中表达均高于人正常甲状腺细胞HT-ori3，差异有统计学意义(

0.05)。与转染siNC相比，PTC细胞株TPC-1和KAT-5在转染siLSINCT5-1、siLSINCT5-2后，TPC-1和KAT-5细胞的增殖和迁移能力均显著降低(

0.05)。双荧光素酶报告基因和RIP证实LSINCT5能够与miR-29c特异性结合。与转染LSINCT5组相比，共转染LSINCT5和miR-29c组中TPC-1细胞增殖和迁移能力均显著降低(

0.05)，ITGB1 mRNA和蛋白表达水平显著下调(

0.05)，E-cadherin蛋白表达水平上升。

结论

LSINCT5能够发挥miRNAs分子海绵的功能，靶向结合miR-29c而抑制miR-29c-ITGB1轴的抑癌作用，促进PTC的增殖和转移。

Abstract

Objective

To evaluate the role of long non-coding RNA (lncRNA) LSINCT5 in promoting the proliferation and metastasis of papillary thyroid cancer (PTC) and explore the mechanism.

Methods

lncRNA high-throughput chip was employed to screen the differentially expressed lncRNAs between PTC tissues and the adjacent tissues. qRT-PCR was used to detect the expression level of LSINCT5 in paired PTC tissues and adjacent tissues from 32 patients (including 18 male and 14 female patients with a mean age of 57 years) and in PTC and normal human thyroid cell lines. Human PTC cell lines TPC-1 and KAT-5 were transfected with siLSINCT5-1 and siLSINCT5-2 for knocking down LSINCT5 expression

and the changes in cell proliferation and invasion were analyzed using CCK-8 assay and Transwell analysis. Dual luciferase reporter analysis and RIP were used to detect the interaction between LSINCT5 and miR-29c. The mRNA and protein expressions of ITGB1 and E-cadherin in TPC-1 cells co-transfected with LSINCT5 and miR-29c were detected using qRT-PCR and Western blotting.

Results

Compared with the adjacent tissues

PTC tissues showed a significantly increased LSINCT5 expression (

0.05). LSINCT5 expression was also significantly elevated in PTC cell lines compared to normal human thyroid HT-ori3 cells (

0.05). Compared with siNC transfection

transfection with siLSINCT5-1 and siLSINCT5-2 for LSINCT5 knockdown significantly suppressed the proliferation and migration of TPC-1 and AKT-5 cells (

0.05). Dual luciferase reporter and RIP analyses confirmed that LSINCT5 could specifically bind to miR-29c. Compared with LSINCT5 transfection alone

co-transfection with LSINCT5 and mir-29c significantly inhibited the cell proliferation and migration (

0.05)

lowered ITGB1 mRNA and protein expressions (

0.05)

and increased E-cadherin protein expression in TPC-1 cells.

Conclusions

LSINCT5 plays the role of miRNA molecular sponge

and by competitively binding to miR-29c

LSINCT5 attenuates the tumor-inhibiting effect of miR-29c-ITGB1 axis to promote the proliferation and metastasis of PTC.

关键词

Keywords

references

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