Effects of ginsenoside Rg3 on fatigue resistance and skeletal muscle mitochondrial function in rats exposed to a simulated altitude of 5 000 m

Jiadan YANG; Rongfeng XIANG; Qing DAI; Xiaodan LAI

doi:10.16016/j.1000-5404.201807172

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Effects of ginsenoside Rg3 on fatigue resistance and skeletal muscle mitochondrial function in rats exposed to a simulated altitude of 5 000 m

Basic Medicine | 更新时间：2026-01-09

- Effects of ginsenoside Rg3 on fatigue resistance and skeletal muscle mitochondrial function in rats exposed to a simulated altitude of 5 000 m
- Journal of Third Military Medical University Vol. 41, Issue 2, Pages: 110-115(2019)
- 作者机构：
  
  1.400016 重庆，重庆医科大学附属第一医院药学部
  2.400038 重庆，陆军军医大学(第三军医大学)第一附属医院药剂科
- 作者简介：
- 基金信息：
  
  the Basic and Frontier Research Plan of Chongqing(CSTC2015jcyjBX0058);the National Natural Science Found for Young Scholars of China(81603330);the Chinese Medicine Science and Technology Project of Chongqing Science and Technology Commission and Chongqing Health and Family Planning Commission(ZY201802043)
- DOI：10.16016/j.1000-5404.201807172
  CLC： R285.5;R322.74;R339.54
- Received：24 July 2018，
  
  Revised：2018-09-01，
  
  Published：30 January 2019
- 稿件说明：
移动端阅览
Jiadan YANG, Rongfeng XIANG, Qing DAI, et al. Effects of ginsenoside Rg3 on fatigue resistance and skeletal muscle mitochondrial function in rats exposed to a simulated altitude of 5 000 m[J]. Journal of Third Military Medical University, 2019, 41(2): 110-115.
DOI：

Jiadan YANG, Rongfeng XIANG, Qing DAI, et al. Effects of ginsenoside Rg3 on fatigue resistance and skeletal muscle mitochondrial function in rats exposed to a simulated altitude of 5 000 m[J]. Journal of Third Military Medical University, 2019, 41(2): 110-115. DOI： 10.16016/j.1000-5404.201807172.

摘要

目的

观察人参皂苷Rg3在模拟高原环境下提高大鼠的抗疲劳能力和对骨骼肌线粒体功能的影响。

方法

40只大鼠按随机数字表法分成4组，每组10只，分为正常对照组、Rg3+正常对照组(NG+Rg3)、缺氧模型组(model group

MG)、Rg3+缺氧模型组(MG+Rg3)。MG组和MG+Rg3组在模拟海拔5 000 m高度的低压舱中喂养，大鼠每日从模拟高原环境中取出1 h行Rg3灌胃，剂量为20 mg/(kg·d)。连续灌胃15 d后，观察大鼠在正常环境和模拟高原环境下力竭游泳时间。力竭游泳实验结束后30 min，腹主动脉采血并检测大鼠总胆固醇(total cholesterol，TC)、甘油三酯(triglyceride，TG)、乳酸脱氢酶(lactate dehydrogenase，LDH)、血糖(glucose

GLU)、血氨(blood ammonia，BA)和血尿素氮(blood urea nitrogen，BUN)的变化。同时迅速分离大鼠股四头肌，检测骨骼肌线粒体丙二醛(malon-dialdehyde

MDA)、锰超氧化物歧化酶(Mn-superoxide dismutase

Mn-SOD)以及呼吸链复合物Ⅲ、Ⅳ活性的变化。

结果

Rg3显著延长正常环境和模拟高原环境下大鼠的力竭游泳时间(

0.01)；显著上调正常环境和模拟高原环境下大鼠血清TC、TG、LDH、GLU浓度(

0.05，

0.01)和骨骼肌线粒体Mn-SOD活性及骨骼肌线粒体呼吸链复合物Ⅲ、Ⅳ活性(

0.01)；显著下调模拟高原环境下大鼠的BUN含量和骨骼肌线粒体MDA含量(

0.01)。

结论

人参皂苷Rg3在模拟高原环境下可延长大鼠力竭游泳时间，改善疲劳相关的生化指标，提高骨骼肌线粒体对自由基的消除作用和供能效力。

Abstract

Objective

To observe the effects of ginsenoside Rg3 (Rg3) in improving fatigue resistance and functionality of skeletal muscle mitochondria in rats at a simulated high altitude.

Methods

Forty adult male SD rats were randomly divided into 4 groups (

=10)

namely the normal group (NG)

Rg3-treated normal group (NG+Rg3)

model group (MG)

and Rg3-treated model group (MG+Rg3). In the latter 2 groups

the rats were exposed to a stimulated altitude of 5 000 m in a hypobaric chamber

and on a daily basis

the rats were taken from the chamber for intragastric administration of 20 mg/kg Rg3 (completed in 1 h). After 15 d of treatment

forced swimming test was performed and 30 min later blood samples were collected from the abdominal aorta for testing the levels of total cholesterol (TC)

plasma triglyceride (TG)

lactate dehydrogenase (LDH)

blood glucose (GLU)

blood ammonia (BA) and blood urea nitrogen (BUN); The quadriceps were quickly isolated for detecting malondialdehyde (MDA)

Mn-superoxide dismutase (Mn-SOD) and the activity of compound Ⅲ and Ⅳ in the respiratory chain.

Results

Rg3 treatment significantly increased the swimming time (

0.05 or 0.01) and serum levels of TC

LDH and GLU (

0.05 or 0.01) and enhanced Mn-SOD activity and mitochondrial respiratory chain complex Ⅲ and Ⅳ activities (

0.01) both in rats kept in normal conditions and in those exposed to the simulated high altitude. Rg3 also significantly decreased serum BUN level and MDA content in the skeletal muscle mitochondria in rats exposed to the simulated high altitude (

0.01).

Conclusion

Rg3 can extend forced swimming time

improve the biochemical indexes related to fatigue

and enhance free radical clearance and energy supply of skeletal muscle mitochondria of rats.

关键词

Keywords

references

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