Effects of carbon, silver and nitrogen ions-implanted silicone rubber on biological behaviors of rat bone marrow mesenchymal stem cells
- Journal of Third Military Medical University Vol. 40, Issue 23, Pages: 2162-2167, 2189(2018)
- 作者机构：
400037 重庆, 陆军军医大学(第三军医大学)第二附属医院整形美容外科
- 作者简介：
- 基金信息：the General Program of National Natural Science Foundation of China(81571918)
DOI：10.16016/j.1000-5404.201807117
CLC： R318.08;R329.21;R329.28Received：10 July 2018，
Revised：2018-08-06，
Published：15 December 2018
- 稿件说明：
移动端阅览
Xiang LI, Yao CHEN, Xin ZHOU, et al. Effects of carbon, silver and nitrogen ions-implanted silicone rubber on biological behaviors of rat bone marrow mesenchymal stem cells[J]. Journal of Third Military Medical University, 2018, 40(23): 2162-2167, 2189.
Xiang LI, Yao CHEN, Xin ZHOU, et al. Effects of carbon, silver and nitrogen ions-implanted silicone rubber on biological behaviors of rat bone marrow mesenchymal stem cells[J]. Journal of Third Military Medical University, 2018, 40(23): 2162-2167, 2189. DOI： 10.16016/j.1000-5404.201807117.
摘要
目的
2
探讨碳、银和氮离子注入硅橡胶对大鼠骨髓间充质干细胞黏附、增殖和分化的影响。
方法
2
以浓度为1×10
16
ions/cm
2
在10KeV条件下向普通硅橡胶注入碳离子、银离子和氮离子
得到碳硅胶(C-SR)、银硅胶(Ag-SR)和氮硅胶(N-SR)3组改性的硅橡胶材料并作为实验组
普通硅橡胶(SR)作为对照组
从130g成年SD大鼠骨髓取2~5代骨髓间充质干细胞进行生物相容性实验。通过CCK-8观察细胞增殖
并在普通光镜下比较黏附细胞的数量和形态
F-actin免疫荧光染色观察细胞骨架形态的变化、Western blot检测成骨及成脂分化相关标志物的表达。
结果
2
成功将离子注入硅橡胶中
得到碳硅胶(C-SR)、银硅胶(Ag-SR)和氮硅胶(N-SR)3种改性的硅橡胶材料。大鼠骨髓间充质干细胞能较好地粘附在改性后的硅橡胶材料上
并且N-SR上细胞增殖较明显
与硅橡胶上的细胞增殖差异有统计学意义(
P
<
0.05)。通过F-actin免疫荧光染色
不同材料上的细胞骨架形态发生变化
Western blot检测结果显示N-SR有促进成骨分化的趋势。
结论
2
碳、银和氮离子注入硅橡胶提高了材料表面的生物相容性
其中N-SR能促进细胞的粘附与增殖
并对干细胞的分化产生影响。
Abstract
Objective
2
To investigate the effects of carbon
silver and nitrogen ions-implanted silicone rubber on the adhesion
proliferation and differentiation of rat bone marrow mesenchymal stem cells (BMMSCs).
Methods
2
The surface of the virgin silicone rubber was implanted with carbon ions
silver ions and nitrogen ions under the condition of 10 KeV at 1×10
16
ions/cm
2
to obtain carbon silica gel (C-SR)
silver silica gel (Ag-SR) and nitrogen silica gel (N-SR). And these gels served as the experimental groups
while the virgin silicone rubber as control group. The BMMSCs were isolated from the 130 g adult SD rats
then the P2 to P5 generation cells were cultured on all above materials. Then CCK-8 assay was used to observe the proliferation of cells
and the number and morphology of adherent cells were observed under a photomicrographe. F-actin immunofluorescence staining was used to observe the change of cytoskeleton
and Western blot assay was used to detect the differentiation of stem cells.
Results
2
The carbon
silver and nitrogen ions were successfully implanted into virgin silicone rubber
and the obtained 3 kinds of modified silicone rubber materials were named as C-SR
Ag-SR and N-SR
respectively. When the rat BMMSCs were cultured on the ion-implanted silicone rubber materials
the cells better adhered to the materials
especially on N-SR. Meanwhile
the cells growing on the N-SR material showed better proliferation
with significant difference with those growing on virgin silicone rubber (
P
<
0.05). The results of F-actin immunofluorescence staining indicated that the cytoskeleton morphology on ion-implanted silicone rubber materials had changed
and Western blot results identified that the BMMSCs had a tendency towards osteogenic differentiation.
Conclusion
2
The carbon
silver and nitrogen ions-implanted silicone rubber materials improve the biocompatibility of the surface of the silicone rubber. N-SR material can promote the adhesion and proliferation and influence the differentiation of the stem cells.
关键词
Keywords
references
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