Molecular epidemiological analysis of three human adenovirus type 55 outbreaks in military forces stationed in Chengdu area

Wenbo WANG; Liangqi GU; Yuan LIU; Yang YANG; Xiuhua ZHAO; Yifan ZHOU; Xuelian ZHANG; Maomao CHEN; Wei QIU; Quanshui FAN; Xiaobing HU

doi:10.16016/j.1000-5404.201807082

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Molecular epidemiological analysis of three human adenovirus type 55 outbreaks in military forces stationed in Chengdu area

Military Medicine | 更新时间：2026-01-09

- Molecular epidemiological analysis of three human adenovirus type 55 outbreaks in military forces stationed in Chengdu area
- Journal of Third Military Medical University Vol. 40, Issue 24, Pages: 2211-2216(2018)
- 作者机构：
  
  1.610021 成都, 西部战区疾病预防控制中心
  2.610083 成都, 西部战区总医院检验科
- 作者简介：
- 基金信息：
  
  the China Postdoctoral Science Fund(2018T111157);the China Postdoctoral Science Fund(2017M623427);the China Postdoctoral Science Fund(2017M613427);the China Postdoctoral Science Fund(2017T100820)
- DOI：10.16016/j.1000-5404.201807082
  CLC： R181.81; R372; R511.8
- Received：11 July 2018，
  
  Revised：2018-09-25，
  
  Published：30 December 2018
- 稿件说明：
移动端阅览
Wenbo WANG, Liangqi GU, Yuan LIU, et al. Molecular epidemiological analysis of three human adenovirus type 55 outbreaks in military forces stationed in Chengdu area[J]. Journal of Third Military Medical University, 2018, 40(24): 2211-2216.
DOI：

Wenbo WANG, Liangqi GU, Yuan LIU, et al. Molecular epidemiological analysis of three human adenovirus type 55 outbreaks in military forces stationed in Chengdu area[J]. Journal of Third Military Medical University, 2018, 40(24): 2211-2216. DOI： 10.16016/j.1000-5404.201807082.

摘要

目的

对成都地区驻地部队暴发55型腺病毒进行病原分离、全基因序列及和遗传进化特点等分子流行病学分析，为揭示55型腺病毒的进化起源及制订疾病防控策略提供参考数据。

方法

采集55型腺病毒感染者咽拭子标本，采用人喉癌上皮细胞HEp-2分离病毒株。利用分片段PCR和测序获取毒株的全基因序列，检索并下载GenBank数据库中55型腺病毒全基因序列，采用生物信息学分析软件-MEGA6.06进行序列比对和系统发育进化树构建。

结果

从成都周边驻地部队3起感染疫情中分离出55型腺病毒5株，分别为24-1株、24-2株、1804001株、1806003株、DYN株。采用12片段分段PCR和测序获得了5株病毒全基因序列，GenBank登录号分别为MH256653~MH256657，5株病毒之间序列差异较小，仅有16处碱基变异。与我国报道的第一株55型腺病毒QS-DLL株和重庆株(CQ-2903)序列相比，有30处碱基变异。根据系统发育进化树分析结果，新分离的5株HAdV-55毒株均与QS-DLL株相近，与我们之前分离的西藏株(LS89/Tibet/2016)、云南株(KM03/YN/2016)等进化距离相对较远。

结论

本研究分析发现2018年成都周边驻地部队流行的55型腺病毒为QS-DLL类似株或重庆分离株CQ-2903株的突变株。

Abstract

Objective

To conduct a molecular epidemiological analysis of 3 human adenovirus type 55 (HAdV-55) outbreaks in the year 2018 in military forces in Chengdu area and obtain epidemiological evidence to identify the evolutionary origin of HAdV-55 for formulating disease prevention and control strategies.

Methods

Throat swab specimens from HAdV-55-infected individuals were collected for virus isolation

via

HEp-2 cells. The full-length DNA sequences of the isolated HAdV-55 strains were obtained with segmented PCR and sequencing. The whole genome sequences of HAdV-55 retrieved from the GenBank database were used for sequence alignment and phylogenetic tree construction using bioinformatics analysis software MEGA6.06.

Results

Five strains of HAdV-55

namely 24-1 strain

24-2 strain

1804001 strain

1806003 strain

and DYN strain

were isolated from 3 outbreaks in the military forces stationed around Chengdu City. The whole genome sequences of these 5 HAdV-55 strains (GenBank accession numbers MH256653

MH256654

MH256655

MH256656

and MH256657

respectively) obtained by 12-segment fragmentation PCR sequencing showed only minor variations with mutations of 16 bases. Compared with the sequence of the first isolated HAdV-55 strain (QS-DLL) in China

these strains showed mutations at 30 bases. Phylogenetic tree analysis showed that the 5 newly isolated HAdV-55 strains were similar to the QS-DLL strain and the Chongqing strain (CQ-2903)

and their evolutionary distances were greater from the previously isolated Tibet strain (LS89/Tibet/2016) and Yunnan strain (KM03/YN/2016).

Conclusion

The HAdV-55 strains prevalent in the 2018 outbreaks in the military forces around Chengdu are QS-DLL-like strains or mutant strains of CQ-2903.

关键词

Keywords

references

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