Role of E3 ubiquitin ligase MARCH5 in proliferation, invasion and migration of cervical cancer cells

Shuai DIAO; Lina HU; Hongtao ZHU; Lim Vincent; Jianguo HU

doi:10.16016/j.1000-5404.201806028

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Role of E3 ubiquitin ligase MARCH5 in proliferation, invasion and migration of cervical cancer cells

Basic Medicine | 更新时间：2026-01-09

- Role of E3 ubiquitin ligase MARCH5 in proliferation, invasion and migration of cervical cancer cells
- Journal of Third Military Medical University Vol. 40, Issue 22, Pages: 2053-2060(2018)
- 作者机构：
  
  400010 重庆, 重庆医科大学附属第二医院妇产科
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Young Scholars of China(81702557)
- DOI：10.16016/j.1000-5404.201806028
  CLC： R345;R730.23;R737.33
- Received：05 June 2018，
  
  Revised：2018-08-13，
  
  Published：30 November 2018
- 稿件说明：
移动端阅览
Shuai DIAO, Lina HU, Hongtao ZHU, et al. Role of E3 ubiquitin ligase MARCH5 in proliferation, invasion and migration of cervical cancer cells[J]. Journal of Third Military Medical University, 2018, 40(22): 2053-2060.
DOI：

Shuai DIAO, Lina HU, Hongtao ZHU, et al. Role of E3 ubiquitin ligase MARCH5 in proliferation, invasion and migration of cervical cancer cells[J]. Journal of Third Military Medical University, 2018, 40(22): 2053-2060. DOI： 10.16016/j.1000-5404.201806028.

摘要

目的

探讨泛素E3连接酶膜相关锌指蛋白5(membrane-associated RING-CH 5，MARCH5)在子宫颈癌组织中的表达、功能以及潜在机制。

方法

用免疫组织化学检测泛素E3连接酶MARCH5在宫颈癌组织中的表达，EdU、Transwell实验和划痕实验检测沉默泛素E3连接酶MARCH5后，宫颈癌细胞增殖、侵袭和迁移能力变化情况，Western blot检测宫颈癌HeLa细胞株沉默MARCH5基因后Drp1、Bax、MARCH5、PGAM的表达情况。使用Mito Tracker Green标记线粒体，然后使用免疫荧光检测MARCH5与线粒体的共定位。

结果

在宫颈癌组织中MARCH5的表达明显较正常宫颈组织高，且MARCH5的表达与宫颈癌的分级有关(

0.01)，与年龄和分期无关(

0.05)。沉默MARCH5后，HeLa细胞的增殖、侵袭和迁移能力降低(

0.05)。沉默MARCH5后，Drp1表达下调，PGAM5和Bax表达上调(

0.05)。

结论

MARCH5在宫颈癌组织中表达上调，是宫颈癌候选癌基因，也是宫颈癌治疗的一个潜在靶点。

Abstract

Objective

To study the expression of membrane-associated RING-CH 5 (MARCH5)

an E3 ubiquitin ligase

in cervical cancer and investigate its role in the proliferation

invasion and migration of cervical cancer cells

in vitro

Methods

Immunohistochemistry was used to detect the expression of MARCH5 in clinical specimens of cervical cancer tissues. The cervical cancer cell line HeLa with MARCH5 silencing was examined for changes in proliferation

invasion

and migration abilities using EdU assay

Transwell assay

and scratch wound healing assay

and Western blotting was used to detect the expression of Drp1

Bax

MARCH5

and PGAM in the cells. Immunofluorescence assay with MitoTracker labeling was employed to characterize the colocalization of MARCH5 with the mitochondria.

Results

Cervical cancer tissues expressed high levels of MARCH5 compared with the normal cervical tissues

and the expression level was correlated with the tumor grade (

0.01) but not with the patients' age or tumor stage (

0.05). Silencing MARCH5 in HeLa cells resulted in significant suppression of the cell proliferation

invasion

and migration (

0.05)

and significantly down-regulated Drp1 expression and up-regulated PGAM5 and Bax expression (

0.05).

Conclusion

MARCH5

which is up-regulated in cervical cancer

can serve as a candidate oncogene for cervical cancer and also a potential target for cervical cancer treatment.

关键词

Keywords

references

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