Effect of capsaicin on hematopoietic stem cell quiescence in mice

Hao ZENG; Mengjia HU; Yong TANG; Yukai LU; Mo CHEN; Junping WANG

doi:10.16016/j.1000-5404.201806015

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Effect of capsaicin on hematopoietic stem cell quiescence in mice

Basic Medicine | 更新时间：2026-01-09

- Effect of capsaicin on hematopoietic stem cell quiescence in mice
- Journal of Third Military Medical University Vol. 40, Issue 22, Pages: 2021-2026(2018)
- 作者机构：
  
  陆军军医大学(第三军医大学)军事预防医学系全军复合伤研究所，创伤、烧伤与复合伤国家重点实验室
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81573084);the Chongqing Science and Technology Innovation Talents Support Program(CSTCKJCXLJRC06)
- DOI：10.16016/j.1000-5404.201806015
  CLC： R285.5;R322.2;R329.25
- Received：03 June 2018，
  
  Revised：2018-06-21，
  
  Published：30 November 2018
- 稿件说明：
移动端阅览
Hao ZENG, Mengjia HU, Yong TANG, et al. Effect of capsaicin on hematopoietic stem cell quiescence in mice[J]. Journal of Third Military Medical University, 2018, 40(22): 2021-2026.
DOI：

Hao ZENG, Mengjia HU, Yong TANG, et al. Effect of capsaicin on hematopoietic stem cell quiescence in mice[J]. Journal of Third Military Medical University, 2018, 40(22): 2021-2026. DOI： 10.16016/j.1000-5404.201806015.

摘要

目的

观察辣椒素对造血干细胞稳态的影响，并初步探讨其相关机制。

方法

采用8~10周龄C57BL/6J小鼠，按随机数字表法分成实验组和对照组，分别给予0.4 mg/d辣椒素溶液和单纯溶剂灌胃建立动物模型。采用流式细胞术检测造血干细胞稳态变化。利用流式分选得到原代造血干细胞进行q-PCR，分析周期相关蛋白激酶CDK2、CDK6 mRNA表达水平。利用CD45.1小鼠作为受体进行造血移植实验。

结果

流式细胞术分析结果显示，实验组造血干/祖细胞(LSK)比例较对照组明显升高[(0.299± 0.041)%

(0.177±0.052)%，

0.01

]

。亚群分析显示，与对照组比较，实验组短期造血干细胞(ST-HSC)比例上升[(47.48±3.22)%

(37.63±1.85)%，

0.01

]

，造血祖细胞(MPP)比例下降[(40.59±3.04)%

(50.12±1.95)%，

0.01

]

。周期分析结果显示，与对照组比较，实验组造血干/祖细胞增殖，G

期细胞数目下降。q-PCR结果也证实实验组CDK2、CDK6 mRNA表达增强。凋亡和竞争性骨髓移植结果两组差异无统计

学意义(

0.05)。

结论

辣椒素促进造血干细胞增殖，灌胃辣椒素能致短期造血功能增强。

Abstract

Objective

To study the effect of capsaicin on the quiescence of hematopoietic stem cells (HSCs) and explore the underlying mechanisms.

Methods

C57BL/6J mice (8-10 weeks old) were randomized for daily intragastric administration of 0.4 mg capsaicin solution or PBS for over 4 weeks. Flow cytometry was used to detect the changes in the HSCs. Fluorescence-activated cell sorting was employed to acquire the primary HSCs for analysis of cycle-related protein kinase CDK2 and CDK6 mRNA expression levels using real-time quantitative PCR (q-PCR). The whole bone marrow cells from capsaicin- or PBS-treated mice were transplanted in mixture with the recipient bone marrow cells in CD45.1 mice

and the hematopoietic capacity of the transplanted HSCs were assessed at different time points after the transplantation.

Results

Flow cytometry analysis showed that capsaicin treatment for over 4 weeks resulted in a significantly increased percentage of hematopoietic stem/ progenitor (LSK) cells in the bone marrow monocytes as compared with PBS treatment [(0.299±0.041)%

(0.177±0.052)%

0.01

]

. Subpopulation analysis of the LSK cells showed that compared with PBS treatment

capsaicin treatment significantly increased the percentage of short-term HSCs [(47.48±3.22)%

(37.63±1.85)%

0.01

]

and decreased the percentage of the hematopoietic progenitor (MPP) cells [(40.59±3.04)%

(50.12±1.95)%

0.01

]

suggesting the enhanced hematopoietic function in capsaicin-treated mice. Cell cycle analysis showed obvious proliferation of LSK cells and a reduction in the G

cells. The results of q-PCR also confirmed the up-regulation of CDK2 and CDK6

mRNA levels in the LSK cells from capsaicin-treated mice. In the cell apoptosis test and competitive bone marrow transplantation experiment

no obvious difference was found between capsaicin and PBS treatment groups

indicating that capsaicin did not affect the long-term hematopoietic capacity of the HSCs.

Conclusion

Capsaicin promotes HSC proliferation

and intragastric administration of capsaicin can enhance the short-term hematopoietic function in mice.

关键词

Keywords

references

YANG F, XIAO X, CHENG W, et al . Structural mechanism underlying capsaicin binding and activation of the TRPV1 ion channel[J ] . Nat Chem Biol, 2015, 11(7): 518-524. DOI: 10.1038/nchembio.1835.

BOGUSZ S Jr, LIBARDI S H, DIAS F F, et al . Brazilian Capsicum peppers: capsaicinoid content and antioxidant activity[J ] . J Sci Food Agric, 2018, 98(1): 217-224. DOI: 10.1002/jsfa.8459.

THOENNISSEN N H, O'KELLY J, LU D, et al . Capsaicin causes cell-cycle arrest and apoptosis in ER-positive and -negative breast cancer cells by modulating the EGFR/HER-2 pathway[J ] . Oncogene, 2010, 29(2): 285-296. DOI: 10.1038/onc.2009.335.

GILARDINI MONTANI M S, D'ELISEO D, CIRONE M, et al . Capsaicin-mediated apoptosis of human bladder cancer cells activates dendritic cells via CD91[J ] . Nutrition, 2015, 31(4): 578-581. DOI: 10.1016/j.nut.2014.05.005.

ZHAO J J, HU Y W, HUANG C, et al . Dihydrocapsaicin suppresses proinflammatory cytokines expression by enhancing nuclear factor IA in a NF-κB-dependent manner[J ] . Arch Biochem Biophys, 2016, 604: 27-35. DOI: 10.1016/j.a bb.2016.06.002.

NISHINO K, TANAMACHI K, NAKANISHI Y, et al . Radiosensitizing effect of TRPV1 channel inhibitors in cancer cells[J ] . Biol Pharm Bull, 2016, 39(7): 1224-1230. DOI: 10.1248/bpb.b16-00080.

BITENCOURT S, STRADIOT L, VERHULST S, et al . Inhibitory effect of dietary capsaicin on liver fibrosis in mice[J ] . Mol Nutr Food Res, 2015, 59(6): 1107-1116. DOI: 10.1002/mnfr.201400649.

王婧, 郎和东, 余利, 等.辣椒素对超重及肥胖成人体成分的影响[J].第三军医大学学报, 2016, 38(14): 1701-1704. DOI: 10.16016/j.1000-5404.201601057.

WANG J, LANG H D, YU L, et al . Effect of dietary capsaicin consumption on body composition in overweight and obese adults[J ] . J Third Mil Med Univ, 2016, 38(14): 1701-1704. DOI: 10.16016/j.1000-5404.201601057.

王瀚, 余智操, 魏卫, 等.辣椒素对微波辐射所致小鼠血液系统损伤的保护作用[J].航天医学与医学工程, 2012, 25(3): 180-183. DOI: 10.16289/j.cnki.1002-0837. 2012.03.009.

WANG H, YU Z C, WEI W, et al . Protective effects of capsaicin on blood system injured by microwave radiation in mice[J ] . Space Med Med Eng, 2012, 25(3): 180-183. DOI: 10.16289/j.cnki. 1002-0837.2012.03.009.

LEE S A, RYU Y S, CHOI H I, et al . Capsaicin promotes the development of burst-forming units-erythroid (BFU-E) from mouse bone marrow cells[J ] . Exp Mol Med, 2007, 39(3): 278-283. DOI: 10.1038/emm.2007.31.

CHALLEN G A, BOLES N, LIN K K, et al . Mouse hemato- poietic stem cell identification and analysis[J ] . Cytometry A, 2009, 75(1): 14-24. DOI: 10.1002/cyto.a.20674.

SUN F, XIONG S, ZHU Z. Dietary capsaicin protects cardio- metabolic organs from dysfunction[J]. Nutrients, 2016, 8(5): 174. DOI: 10.3390/nu8050174.

WANG Z, EMA H. Mechanisms of self-renewal in hematopoietic stem cells[J]. Int J Hematol, 2016, 103 (5): 498-509. DOI: 10.1007/s12185-015-1919-5.

COŞKUN S, CHAO H, VASAVADA H, et al . Development of the fetal bone marrow niche and regulation of HSC quiescence and homing ability by emerging osteolineage cells[J ] . Cell Rep, 2014, 9(2): 581-590. DOI: 10.1016/j.celrep.2014.09.013.

MENDELSON A, FRENETTE P S. Hematopoietic stem cell niche maintenance during homeostasis and regeneration[J]. Nat Med, 2014, 20(8): 833-846. DOI: 10.1038/nm.3647.

YAMADA T,PARK C S, LACORAZZA H D. Genetic control of quiescence in hematopoietic stem cells[J]. Cell Cycle, 2013, 12(15): 2376-2383. DOI: 10.4161/cc.25416.

MAIESE K. Warming up to new possibilities with the capsaicin receptor TRPV1: mTOR, AMPK, and erythropoietin[J]. Curr Neurovasc Res, 2017, 14(2): 184-189. DOI: 10.2174/ 1567202614666170313105337.

KUNISAKI Y, BRUNS I, SCHEIERMANN C, et al . Arteriolar niches maintain haematopoietic stem cell quiescence[J ] . Nature, 2013, 502(7473): 637-643. DOI: 10.1038/nature12612.

RAMKUMAR C, KONG Y, TRABUCCO S E, et al . Smurf2 regulates hematopoietic stem cell self-renewal and aging[J ] . Aging Cell, 2014, 13(3): 478-486. DOI: 10.1111/acel.12195.

YAMASHITA M, NITTA E, SUDA T. Aspp1 preserves hematopoietic stem cell pool integrity and prevents malignant transformation[J]. Cell Stem Cell, 2015, 17(1): 23-34. DOI: 10.1016/j.stem.2015.05.013.

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