Expression and clinical significance of miR-142-5p in esophageal squamous cell carcinoma

Jian XU; Yuequan JIANG; Huarong CAI; Zhi ZHANG; Zhe YIN; Qi ZHANG

doi:10.16016/j.1000-5404.201805133

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Expression and clinical significance of miR-142-5p in esophageal squamous cell carcinoma

更新时间：2026-01-09

- Expression and clinical significance of miR-142-5p in esophageal squamous cell carcinoma
- Journal of Third Military Medical University Vol. 40, Issue 21, Pages: 1936-1941(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201805133
  CLC：
- Received：17 May 2018，
  
  Published：15 November 2018
- 稿件说明：
移动端阅览
Jian XU, Yuequan JIANG, Huarong CAI, et al. Expression and clinical significance of miR-142-5p in esophageal squamous cell carcinoma[J]. Journal of Third Military Medical University, 2018, 40(21): 1936-1941.
DOI：

Jian XU, Yuequan JIANG, Huarong CAI, et al. Expression and clinical significance of miR-142-5p in esophageal squamous cell carcinoma[J]. Journal of Third Military Medical University, 2018, 40(21): 1936-1941. DOI： 10.16016/j.1000-5404.201805133.

摘要

Abstract

Objective

To investigate the expression and clinical significance of miR-142-5p in esophageal squamous cell carcinoma (ESCC).

Methods

The cancer and para-cancerous tissue specimens of 100 ESCC patients undergoing surgical treatment in our hospital from April to September 2014 were collected

and examined by real time fluorescence quantitative PCR. And all cases were followed up for at least 3 years. Its mRNA expression was also detected in the ESCC cell lines Eca109 and Kyse150 and normal human esophageal epithelial cell line Het-1. Eca109 cells were transfected with miR-142-5p mimic (miR-142-5p mimic group) or mimics control (negative control)

and those untreated served as blank control. The level of TGF-β2 protein was measured by Western blotting. At the same time

the expression levels of miR-142-5p in ESCC cell lines were detected.

Results

The relative expression level of miR-142-5p was 0.78±0.16 in the ESCC tissues

which was significantly lower than that in the adjacent tissues (1.43±0.34

0.05). Its level in the Eca109 (1.53±0.27) and Kyse150 cells (1.12±0.31) were significantly lower than that in the normal esophageal epithelial cell line Het-1a (2.24±0.68

0.05). The protein level of TGF-β2 protein was significantly lower in the miR-142-5p mimic transfected cells than the negative and blank control cells (

0.05). The expression level of miR-142-5p in the ESCC tissues was correlated with the lymph node metastasis

tumor stage

and history of alcohol consumption (

0.05). The time of progression was obviously shorter in the patients with declined miR-142-5p expression than those without (

0.05).

Conclusion

MiR-142-5p is involved in the occurrence of ESCC

and its low expression is related to the invasion

metastasis

and prognosis of ESCC patients.

关键词

Keywords

references

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