Resveratrol protects human umbilical vein endothelial cells against oxidative injury by activating transcription factor EB <italic style="font-style: italic">in vitro</italic>

Xi ZHOU; Long YI; Min ZHOU; Yu ZHANG; Pengfei HOU; Yang LIU; Mantian MI

doi:10.16016/j.1000-5404.201805059

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Resveratrol protects human umbilical vein endothelial cells against oxidative injury by activating transcription factor EB in vitro

Basic Medicine | 更新时间：2026-01-09

- Resveratrol protects human umbilical vein endothelial cells against oxidative injury by activating transcription factor EB in vitro
- Journal of Third Military Medical University Vol. 40, Issue 20, Pages: 1839-1845(2018)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)军事预防医学系营养与食品安全研究中心，重庆市医学营养研究中心，重庆市营养与食品安全重点实验室
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Young Scholars of China(81502804)
- DOI：10.16016/j.1000-5404.201805059
  CLC： R282.71;R285.5;R543
- Received：08 May 2018，
  
  Revised：2018-07-18，
  
  Published：30 October 2018
- 稿件说明：
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Xi ZHOU, Long YI, Min ZHOU, et al. Resveratrol protects human umbilical vein endothelial cells against oxidative injury by activating transcription factor EB in vitro[J]. Journal of Third Military Medical University, 2018, 40(20): 1839-1845.
DOI：

Xi ZHOU, Long YI, Min ZHOU, et al. Resveratrol protects human umbilical vein endothelial cells against oxidative injury by activating transcription factor EB in vitro[J]. Journal of Third Military Medical University, 2018, 40(20): 1839-1845. DOI： 10.16016/j.1000-5404.201805059.

摘要

目的

观察白藜芦醇(resveratrol，RSV)对血管内皮细胞氧化应激损伤的抑制作用，并探索转录因子EB(transcription factor EB

TFEB)在其中的影响。

方法

用棕榈酸(palmitate，PA)处理原代人脐静脉内皮细胞(human umbilical vein endothelial cells，HUVECs)建立氧化应激损伤模型。RSV(10 μmol/L)预处理2 h，再用PA(200 μmol/L)处理24 h，CCK-8法检测细胞增殖活力，荧光酶标仪检测细胞内活性氧(ROS)生成，脂质氧化丙二醛(malondialdehyde

MDA)试剂盒检测细胞脂质过氧化水平。Western blot检测RSV对TFEB蛋白表达的影响。

结果

PA(200 μmol/L)处理24 h可显著抑制内皮细胞增殖活力，上调胞内ROS水平和MDA水平，导致内皮细胞氧化应激损伤(

0.01)。RSV预处理后能显著抑制PA诱导的氧化应激损伤，显著上调TFEB的mRNA和蛋白表达(

0.05)，而TFEB siRNA处理以后，RSV的保护性作用被明显削弱。

结论

白藜芦醇是通过激活转录因子EB，从而抑制棕榈酸诱导的内皮细胞氧化应激损伤。

Abstract

Objective

To observe the inhibitory effect of resveratrol on oxidative stress in human umbilical vein endothelial cells (HUVECs)

in vitro

and explore the role of transcription factor EB (TFEB) in mediating the anti-oxidative effect of resveratrol.

Methods

Primary culture of HUVECs was pretreated with 10 μmol/L resveratrol for 2 h followed by exposure to 200 μmol/L palmitate for 24 h. The proliferation of the cells was assessed using CCK-8 assay

and reactive oxygen species (ROS) and malondialdehyde (MDA) levels in the cells were determined using a commercial detection kit. The mRNA and protein expressions of TFEB were measured using qRT-PCR and Western blotting

respectively.

Results

Palmitate treatment significantly decreased the viability and increased ROS and MDA levels in cultured HUVECs (

0.01). Pretreatment with resveratrol significantly inhibited PA-induced oxidative injury and up-regulated the mRNA and protein levels of TFEB in the cells; knockdown of TFEB with a specific siRNA obviously attenuated the protective effect of resveratrol against oxidative cell injury (

0.05).

Conclusions

Resveratrol can protect cultured HUVECs against oxidative injury by activating TFEB.

关键词

Keywords

references

CHANG P P, WRUCK L M, SHAHAR E, et al. Trends in hospitalizations and survival of acute decompensated heart failure in four US communities (2005-2014): ARIC study community surveillance[J]. Circulation, 2018, 138(1): 12-24. DOI: 10.1161/CIRCULATIONAHA.117.027551.

SHAW L J, GOYAL A, MEHTA C, et al. 10-Year resource utilization and xosts for cardiovascular care[J]. J Am Coll Cardiol, 2018, 71(10): 1078-1089. DOI: 10.1016/j.jacc. 2017.12.064.

SCHOBER A, WEBER C. Mechanisms of microRNAs in atherosclerosis[J]. Annu Rev Pathol, 2016, 11: 583-616. DOI: 10.1146/annurev-pathol-012615-044135.

TAJBAKHSH A, REZAEE M, KOVANEN P T, et al. Efferocytosis in atherosclerotic lesions: malfunctioning regulatory pathways and control mechanisms[J]. Pharmacol Ther, 2018, 188: 12-25. DOI: 10.1016/j.pharmthera.2018.02.003.

FÖRSTERMANN U, XIA N, LI H. Roles of vascular oxidative stress and nitric oxide in the pathogenesis of atherosclerosis[J]. Circ Res, 2017, 120(4): 713-735. DOI: 10.1161/CIRCRESAHA.116.309326.

ZHOU X, CHEN M, ZENG X, et al. Resveratrol regulates mitochondrial reactive oxygen species homeostasis through Sirt3 signaling pathway in human vascular endothelial cells[J]. Cell Death Dis, 2014, 5: e1576. DOI: 10.1038/cddis.2014.530.

苏倩. Akt信号通路通过激活TFEB而抑制氧化应激诱导的神经细胞凋亡[D]. 石家庄: 河北医科大学, 2017.

SU Q. Activation of Akt signaling pathway suppresses oxidative stress-induced neuronal apoptosis through phosphorylating TFEB[D]. Shijiazhuang: Hebei Medical University, 2017.

徐得莱. 转录因子EB在氧化应激诱导细胞自噬中的作用[D]. 苏州: 苏州大学, 2016.

XU D L. The role of transcription factor EB in oxidative stress induced cell autophagy[D]. Suzhou: Soochow University, 2016.

SETTEMBRE C, DE CEGLI R, MANSUETO G, et al.TFEB controls cellular lipid metabolism through a starvation-induced autoregulatory loop[J]. Nat Cell Biol, 2013, 15(6): 647-658. DOI: 10.1038/ncb2718.

SETTEMBRE C, DI MALTA C, POLITO V A, et al. TFEB links autophagy to lysosomal biogenesis[J]. Science, 2011, 332(6036): 1429-1433. DOI: 10.1126/science.1204592.

SARDIELLO M, PALMIERI M, DI RONZA A, et al. a gene network regulating lysosomal biogenesis and function[J]. Science, 2009, 325(5939): 473-477. DOI: 10.1126/science.1174447.

CHEN M L, YI L, JIN X, et al. Resveratrol attenuates vascular endothelial inflammation by inducing autophagy through the cAMP signaling pathway[J]. Autophagy, 2013, 9(12): 2033-2045. DOI: 10.4161/auto.26336.

周曦, 易龙, 金鑫, 等. SIRT1/UCP2通路在白藜芦醇抑制血管内皮细胞氧化应激损伤中的作用[J].第三军医大学学报, 2013, 35(16): 1671-1675.

ZHOU X, YI L, JIN X, et al. Role of SIRT1/UCP2 signaling pathway in resveratrol-induced inhibition of oxidative injure in vascular endothelial cells[J]. J Third Mil Med Univ, 2013, 35(16): 1671-1675.

ZHU X D, LEI X P, DONG W B. Resveratrol as a potential therapeutic drug for respiratory system diseases[J]. Drug Des Devel Ther, 2017, 11: 3591-3598. DOI: 10.2147/DDDT.S148868.

POLLACK R M, BARZILAI N, ANGHEL V, et al. Resveratrol improves vascular function and mitochondrial number but not glucose metabolism in older adults[J]. J Gerontol a Biol Sci Med Sci, 2017, 72(12): 1703-1709. DOI: 10.1093/gerona/glx041.

LI Y R, LI S, LIN C C. Effect of resveratrol and pterostilbene on aging and longevity[J]. Biofactors, 2018, 44(1): 69-82. DOI: 10.1002/biof.1400.

BONKOWSKI M S, SINCLAIR D A. Slowing ageing by design: the rise of NAD+ and sirtuin-activating compounds[J]. Nat Rev Mol Cell Biol, 2016, 17(11): 679-690. DOI: 10.1038/nrm.2016.93.

PETROVSKI G, GURUSAMY N, DAS D K. Resveratrol in cardiovascular health and disease[J]. Ann N Y Acad Sci, 2011, 1215: 22-33. DOI: 10.1111/j.1749-6632.2010.05843.x.

CSISZAR A. Anti-inflammatory effects of resveratrol: possible role in prevention of age-related cardiovascular disease[J]. Ann N Y Acad Sci, 2011, 1215: 117-122. DOI: 10.1111/j.1749-6632.2010.05848.x.

MORSELLI E, MAIURI M C, MARKAKI M, et al. Caloric restriction and resveratrol promote longevity through the sirtuin-1-dependent induction of autophagy[J]. Cell Death Dis, 2010, 1: e10. DOI: 10.1038/cddis.2009.8.

KIM M Y, LIM J H, YOUN h H, et al. Resveratrol prevents renal lipotoxicity and inhibits mesangial cell glucotoxicity in a manner dependent on the AMPK-SIRT1-PGC1α axis in db/db mice[J]. Diabetologia, 2013, 56(1): 204-217. DOI: 10. 1007/s00125-012-2747-2.

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Resveratrol protects human umbilical vein endothelial cells against oxidative injury by activating transcription factor EB in vitro

DOI：10.16016/j.1000-5404.201805059

摘要

Abstract

关键词

Keywords

references