Expression, clinical significance and functional roles of LHX3 gene in hepatocellular carcinoma

Zhonglan YOU; Zhanfei TIAN; Yi HONG; Yan ZHU; Yazhou WU

doi:10.16016/j.1000-5404.201804071

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Expression, clinical significance and functional roles of LHX3 gene in hepatocellular carcinoma

Basic Medicine | 更新时间：2026-01-09

- Expression, clinical significance and functional roles of LHX3 gene in hepatocellular carcinoma
- Journal of Third Military Medical University Vol. 40, Issue 19, Pages: 1741-1747(2018)
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)
  2.军事预防医学系卫生统计学教研室，第一附属医院感染科
  3.430010 武汉，解放军第161医院感染科
  4.646000 四川泸州，泸州市人民医院感染科
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81573254);the Natural Science Foundation of Chongqing(CSTC2018jcyjAX0473)
- DOI：10.16016/j.1000-5404.201804071
  CLC： R363.2+5;R394.2;R735.7
- Received：11 April 2018，
  
  Revised：2018-06-11，
  
  Published：15 October 2018
- 稿件说明：
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Zhonglan YOU, Zhanfei TIAN, Yi HONG, et al. Expression, clinical significance and functional roles of LHX3 gene in hepatocellular carcinoma[J]. Journal of Third Military Medical University, 2018, 40(19): 1741-1747.
DOI：

Zhonglan YOU, Zhanfei TIAN, Yi HONG, et al. Expression, clinical significance and functional roles of LHX3 gene in hepatocellular carcinoma[J]. Journal of Third Military Medical University, 2018, 40(19): 1741-1747. DOI： 10.16016/j.1000-5404.201804071.

摘要

目的

探讨LHX3基因在肝癌患者中的表达、临床和预后意义以及生物学功能。

方法

收集具有完整临床病理信息的肝癌患者组织样本100例和对应的癌旁组织45例制备成组织芯片。采用PCR、Western blot和免疫组织化学方法分析LHX3基因在肝癌组织和癌旁/正常肝组织中的表达变化，分析LHX3在不同组织中的表达差异、LHX3基因表达与患者临床病理参数之间的关系。采用Kaplan-Meier和Cox regression生存分析法确定LHX3基因表达在肝癌患者总生存时间中的意义。通过Transwell实验分析LHX3基因在肝癌转移中的功能。

结果

与癌旁或正常组织相比，LHX3基因在肝癌组织中的表达显著升高；LHX3表达与肝癌患者的临床分期和肿

瘤转移密切相关(

0.05，

0.01)，而与患者的年龄、性别、肿瘤体积、病理分级和肿瘤位置不相关(

0.05)；而且LHX3基因在临床晚期患者和转移患者中的表达均出现了急剧升高；生存分析发现LHX3高表达与患者不良预后紧密相关(

=0.002)，而且是一个独立的预后标志物(风险比=1.265，

=0.032)；进一步功能分析结果显示，过表达LHX3基因能够明显促进肿瘤细胞的迁移和侵袭能力。

结论

LHX3基因通过促进肝癌细胞的转移缩短肝癌患者的总生存时间。

Abstract

Objective

To investigate the expression

clinical significant and functional roles of LIM homeobox 3 (LHX3) in hepatocellular carcinoma (HCC).

Methods

Atotal of 100 HCC tissue samples from the patients with fully clinicopathological data and 45 paired para-cancerous tissues were collected for tissue microarray. PCR

Western blotting and immunohistochemical staining were used to detect the expression of LHX3 in the HCC tissues and para-cancerous tissues. The differences of LHX3 expression in different groups and the relationship of the expression with clinicopathological data were analyzed. Kaplan-Meier and Cox regression survival analyses were performed to investigate the prognostic significance of the gene in total survival time. Transwell assay was employed to study the functional roles of LHX3 in tumor metastasis.

Results

LHX3 was highly expressed in the HCC tissues compared to normal or para-cancerous tissues. The expression was significantly correlated with clinical stage (

=0.037) and metastasis (

0.001)

but not with age

gender

histological grade

tumor size and tumor location (

0.05) of HCC patients. Its expression was remarkably increased in the HCC patients at advanced stages or with metastasis. Further survival analyses showed that the increased LHX3 expression level was associated with unfavorable prognosis (

=0.002)

and could be regarded as an independent prognostic factor for HCC patients (haz

ard ratio=1.265

=0.032). Functional studies indicated that LHX3 over-expression promoted cancer cell migration and invasion in HCC cells.

Conclusion

LHX3 shortens the total survival time of HCC patients through promoting metastasis.

关键词

Keywords

references

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