Down-regulation of integrin-linked kinase suppresses glycolysis and proliferation and promotes apoptosis of cervical cancer cells possibly by inhibiting Wnt/β-catenin pathway

Juan SHI; Xia FAN

doi:10.16016/j.1000-5404.201804008

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Down-regulation of integrin-linked kinase suppresses glycolysis and proliferation and promotes apoptosis of cervical cancer cells possibly by inhibiting Wnt/β-catenin pathway

Military Medicine | 更新时间：2026-01-09

- Down-regulation of integrin-linked kinase suppresses glycolysis and proliferation and promotes apoptosis of cervical cancer cells possibly by inhibiting Wnt/β-catenin pathway
- Journal of Third Military Medical University Vol. 40, Issue 18, Pages: 1668-1673(2018)
- 作者机构：
  
  1.716000 陕西延安，延安大学医学院:外妇护理教研室
  2.716000 陕西延安，延安大学医学院:预防医学教研室
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201804008
  CLC： R73-36; R730.51; R737.33
- Received：02 April 2018，
  
  Revised：2018-06-05，
  
  Published：30 September 2018
- 稿件说明：
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Juan SHI, Xia FAN. Down-regulation of integrin-linked kinase suppresses glycolysis and proliferation and promotes apoptosis of cervical cancer cells possibly by inhibiting Wnt/β-catenin pathway[J]. Journal of Third Military Medical University, 2018, 40(18): 1668-1673.
DOI：

Juan SHI, Xia FAN. Down-regulation of integrin-linked kinase suppresses glycolysis and proliferation and promotes apoptosis of cervical cancer cells possibly by inhibiting Wnt/β-catenin pathway[J]. Journal of Third Military Medical University, 2018, 40(18): 1668-1673. DOI： 10.16016/j.1000-5404.201804008.

摘要

目的

观察下调整合素连接激酶(integrin-linked kinase，ILK)对宫颈癌细胞糖酵解及增殖、凋亡的影响，探讨其相关作用机制。

方法

选择宫颈癌细胞CaSki，细胞分3组，转染ILK siRNA、siRNA control分别为干扰组和阴性组，以未转染的细胞为对照组。qRT-PCR、Western blot检测细胞中ILK mRNA、蛋白表达水平。试剂盒检测细胞中丙酮酸激酶(pyruvate kinase，PK)、己糖激酶(hexokinase，HK)、三磷腺苷(adenosine triphosphate，ATP)水平和培养液中乳酸水平，MTT法检测细胞增殖，流式细胞术检测细胞凋亡，Western blot检测细胞中活化的含半胱氨酸的天冬氨酸蛋白水解酶3(cleaved Caspase-3)、活化的含半胱氨酸的天冬氨酸蛋白水解酶9(cleaved Caspase-9)、β-连环蛋白(β-catenin)、c-myc蛋白水平。

结果

ILK siRNA能够下调宫颈癌细胞中ILK mRNA和蛋白水平，siRNA control对细胞中ILK mRNA和蛋白水平没有影响。阴性组PK、HK、ATP、乳酸水平、细胞存活率、凋亡率、cleaved Caspase-3、cleaved Caspase-9、β-catenin、c-myc表达水平与对照组相比差异均无统计学意义(

0.05)。与对照组比较，干扰组PK、HK、ATP、乳酸水平明显降低，细胞存活率明显降低，细胞凋亡率明显升高，细胞中cleaved Caspase-3、cleaved Caspase-9表达水平升高，β-catenin、c-myc表达水平下降，差异均有统计学意义(

0.05)。

结论

下调ILK抑制宫颈癌细胞糖酵解和细胞增殖，促进细胞凋亡，作用机制可能与下调Wnt/β-catenin信号通路的激活有关。

Abstract

Objective

To study the effect of small interfering RNA (siRNA)-mediated down-regulation of integrin-linked kinase (ILK) on glycolysis

proliferation

and apoptosis of cervical cancer cells and explore the underlying mechanism.

Methods

Human cervical cancer CaSki cells were transfected with a specific siRNA targeting ILK and a control siRNA

and the changes in the expression levels of ILK mRNA and protein were detected using qRT-PCR and Western blotting. The levels of pyruvate kinase (PK)

hexokinase (HK)

ATP and lactic acid in the culture medium were detected using a commercial detection kits. The changes in the proliferation and apoptosis of the transfected cells were detected using MTT assay and flow cytometry

respectively

and the expression levels of cleaved Caspase-3

cleaved Caspase-9

β-catenin and c-myc proteins were detected using Western blotting.

Results

Transfection with ILK siRNA

but not the control siRNA

significantly reduced ILK mRNA and protein expressions in the cells. Transfection of the cells with ILK siRNA caused significant reduction in the levels of PK

ATP and lactic acid in the culture medium

significantly decreased cell survival rate

increased apoptosis rate

enhanced the levels of cleaved Caspase-3 and Cleaved caspase-9

and lowered the expressions of β-catenin and c-myc as compared with the control cells (

0.05).

Conclusion

Down-regulation of ILK inhibits glycolysis and cell growth and promotes cell apoptosis of cervical cancer cells in vitro possibly by inhibiting the activation of Wnt/β-catenin signaling pathway.

关键词

Keywords

references

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