Beneficial effects of a new hemoglobin substitute on functions of vital organs in traumatic hemorrhagic shock rats

Yu ZHU; Kunlun TIAN; Yue WU; Jie ZHANG; Xiaoyong PENG; Xinming XIANG; Liangming LIU; Tao LI

doi:10.16016/j.1000-5404.201803174

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Beneficial effects of a new hemoglobin substitute on functions of vital organs in traumatic hemorrhagic shock rats

Military Medicine | 更新时间：2026-01-09

- Beneficial effects of a new hemoglobin substitute on functions of vital organs in traumatic hemorrhagic shock rats
- Journal of Third Military Medical University Vol. 40, Issue 19, Pages: 1729-1734(2018)
- 作者机构：
  
  400042 重庆，陆军军医大学(第三军医大学)第三附属医院(野战外科研究所)第二研究室，创伤、烧伤与复合伤国家重点实验室
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Young Scholars of China(81700429)
- DOI：10.16016/j.1000-5404.201803174
  CLC： R641;R657.62;R973.5
- Received：15 March 2018，
  
  Revised：2018-07-02，
  
  Published：15 October 2018
- 稿件说明：
移动端阅览
Yu ZHU, Kunlun TIAN, Yue WU, et al. Beneficial effects of a new hemoglobin substitute on functions of vital organs in traumatic hemorrhagic shock rats[J]. Journal of Third Military Medical University, 2018, 40(19): 1729-1734.
DOI：

Yu ZHU, Kunlun TIAN, Yue WU, et al. Beneficial effects of a new hemoglobin substitute on functions of vital organs in traumatic hemorrhagic shock rats[J]. Journal of Third Military Medical University, 2018, 40(19): 1729-1734. DOI： 10.16016/j.1000-5404.201803174.

摘要

目的

观察一种新型血红蛋白代用品，产品代号YQ，对非控失血性休克大鼠重要器官的保护作用。

方法

100只SD大鼠[雌雄各半，12周龄，体质量(220±20)g

]

随机分为(

=20)：0.5%YQ组、2%YQ组、5%YQ组、乳酸林格氏液(lactate Ringer，LR)对照组和全血组。采用切断脾动脉分支方法构建非控失血性休克模型，观察不同浓度YQ在不同复苏阶段(复苏血压维持50、70、80 mmHg，复苏后1、2 h等)对重要脏器(肝、肾)血流量、组织氧分压、器官功能及血气的影响。

结果

在不同复苏阶段，与LR对照组比较：①3个YQ浓度均可增加大鼠的PaO

和SaO

。复苏2 h后2%YQ[(98.8±15.9)mmHg

]

和5%YQ组[(98.6±14.1) mmHg

]

对PaO

的作用明显优于LR对照组[(68.6±5.6)mmHg

]

(

0.01)；2%YQ[(92.1±2.5) mmHg

]

和5%YQ组[(93.6±2.4) mmHg

]

对SaO

的作用明显优于LR对照组[(70.6±8.5)mmHg

]

(

0.01)，接近全血[(94.3±3.3) mmHg

]

。②3个YQ浓度均可增加经皮氧分压，其中5%YQ组效果最好，复苏2 h后其作用[(80.4±5.9) mmHg

]

接近全血组[(80.0±7.3) mmHg

]

，与LR对照组比较[(59.1±1.6) mmHg

]

，有显著性差异(

0.01)。③3个YQ浓度均可增加大鼠的肝肾血流量，其中5%YQ组效果最好，复苏2 h后其作用[肝：(218.7±13.8) U/min；肾：(187.4±10.9) U/min

]

接近全血组[肝：(220.4±18.2) U/min；肾：(179.2±16.3) U/min

]

，明显优于LR对照组[肝：(171.4±20.4) U/min；肾：(169.4±4.7) U/min

]

。④3个YQ浓度组均可降低大鼠AST、ALT、BUN和Crea水平。与LR对照组比较[AST：(209.6±15.6) U/L；ALT：(54.9±3.6) U/L；BUN：(10.8±2.5) mmol/L；Crea：(47.7±4.4) μmol/L

]

，复苏2 h后5%YQ组的肝肾功能[AST：(149.8±13.7)U/L；ALT：(45.9±4.2) U/L；BUN：(9.7±1.8) mmol/L；Crea：(25.8±2.1) μmol/L

]

明显减低。

结论

YQ通过改善肝、肾器官功能从而发挥对非控制失血性休克大鼠的保护作用。

Abstract

Objective

To investigate the effects of a new type hemoglobin substitute (product code YQ) on main organ functions in uncontrolled hemorrhagic shock rats.

Methods

A total of 100 SD rats (50 males and 50 females

at an age of 12 weeks

body weight 220±20 g) were randomly divided into 5 groups: 0.5%YQ

2.0%YQ

5%YQ

lactate Ringer's solution (LR) group

and whole blood group

with 20 rats in each group. After the uncontrolled hemorrhagic shock model was established in the rats by cutting off the splenic artery branch

the effects of different concentrations of YQ on vital organ blood flow (liver and kidneys)

functions of the liver and kidneys

and transcutaneous partial pressures of oxygen and blood gases were tested at vary resuscitation stages (resuscitation being maintained at 50

70 and 80 mmHg

in 1 and 2 h after resuscitation).

Results

① Compared with LR

all 3 doses of YQ increased PaO

and SaO

at different resuscitation stages; At 2 h post-resuscitation stage

the 2% YQ (98.8±15.9 mmHg) and 5% YQ groups (98.6±14.1 mmHg) showed better effects on PaO

than the LR group (68.6±5.6 mmHg

0.01)

while so were their effects on SaO

(92.1±2.5 and 93.6±2.4

70.6±8.5 mmHg

0.01)

which was similar to that of the whole blood group (94.3±3.3 mmHg). ② All the 3 doses of YQ enhanced the transcutaneous oxygen pressures at different resuscitation stages

and 5% YQ presented the best effect

with its effect closed to that of the whole blood group at 2 h post-resuscitation stage (80.4±5.9

80.0±7.3 mmHg)

while significantly higher than that of the LR group (59.1±1.6 mmHg

0.01). ③ The liver and kidney blood flows were elevated in all the 3 YQ groups at different resuscitation stages. Among them

the 5% YQ had the best effect

with the effects equivalent to those of the whole blood group at 2 h post-resuscitation stage (liver: 218.7±13.8

220.4±18.2 U/min; kidneys: 187.4±10.9

179.2±16.3 U/min) and better than those of the LR group (liver: 171.4±20.4 U/min; kidneys: 169.4±4.7 U/min). ④ The levels of AST

ALT

BUN and Crea were all reduced in the 3 YQ groups at different resuscitation stages. The 5% YQ group had significantly decreased AST (209.6±15.6

149.8±13.7 U/L)

ALT (54.9±3.6

45.9±4.2 U/L)

BUN (10.8±2.5

9.7±1.8 mmol/L) and Crea (47.7±4.4

25.8±2.1 μmol/L) when compared with the LR group at 2 h post-resuscitation stage.

Conclusion

YQ exerts protective effect on uncontrolled hemorrhagic shock via improving liver and kidney functions.

关键词

Keywords

references

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