Identification of optimal reference genes during development of hydatid cysts from protoscoleces and transcriptional expression of candidate aquaporins

Fen WANG; Xunuo LIU; Hongye WU; Kai LI; Junjie FAN; Bin YE

doi:10.16016/j.1000-5404.201803049

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Identification of optimal reference genes during development of hydatid cysts from protoscoleces and transcriptional expression of candidate aquaporins

更新时间：2026-01-09

- Identification of optimal reference genes during development of hydatid cysts from protoscoleces and transcriptional expression of candidate aquaporins
- Journal of Third Military Medical University Vol. 40, Issue 17, Pages: 1533-1541(2018)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81672045);the General Program of National Natural Science Foundation of China(30972567);the General Program of National Natural Science Foundation of China(30371258)
- DOI：10.16016/j.1000-5404.201803049
  CLC：
- Received：12 March 2018，
  
  Published：15 September 2018
- 稿件说明：
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Fen WANG, Xunuo LIU, Hongye WU, et al. Identification of optimal reference genes during development of hydatid cysts from protoscoleces and transcriptional expression of candidate aquaporins[J]. Journal of Third Military Medical University, 2018, 40(17): 1533-1541.
DOI：

Fen WANG, Xunuo LIU, Hongye WU, et al. Identification of optimal reference genes during development of hydatid cysts from protoscoleces and transcriptional expression of candidate aquaporins[J]. Journal of Third Military Medical University, 2018, 40(17): 1533-1541. DOI： 10.16016/j.1000-5404.201803049.

摘要

Abstract

Objective

To select suitable reference genes for detecting transcriptional expression profiles of aquaporins genes of

Echinococcus granulosus

(

AQPs) during the development of hydatid cysts from protoscoleces.

Methods

geNorm software was used to evaluate the stability of 5 candidate reference genes

including

ND1

ATP6

elp

actin

and

Act

Ⅱ

and then RT-qPCR was employed to detect the transcriptional expression level of

AQP in

in vitro

protoscoleces and in mice during the development of hydatid cysts from protoscoleces.

Results

Actin

and

Act

Ⅱ were optimal for detecting transcriptional level of

AQP gene in

in vitro

protoscoleces by RT-qPCR. While

ATP6

and

ND1

were optimal for the transcriptional level of the

AQP3 genes in the germinal cells of cyst walls of hydatids developed from protoscoleces

in vivo

. The transcriptional levels of

AQP3 and

AQP9 reached the highest in protoscoleces after 3 days of culture

and then were in

trend of decreasing with the time elapse

with significant difference in different time points (

0.05). The transcriptional expression of

AQP4 was the highest in protoscoleces after 25 days' culture (

0.05)

and comparatively lower in the days 3

15 and 35

though without notably difference (

0.05). In

in vivo

study

the transcriptional levels of

AQP3

AQP4 and

AQP9 were the highest in protoscoleces (

0.05)

and were quite low in 3 to 7 months after the protoscoleces inoculation in the abdominal cavities of mice

but there were no statistical differences (

0.05). The expression levels of

AQP

AQP1

AQPFA-CHIP

and

AQPAnG were very low and even hardly detected in both the protoscoleces and the germinal cells of cyst walls of hydatids developed from protoscoleces.

Conclusion

The optimal reference genes for transcriptional expression are identified by geNorm during the development of hydatid cysts from protoscoleces. The transcriptional levels of

AQPs genes are very low in germinal cells of cyst walls of hydatids developed from protoscoleces

which may be related to the formation or accumulation of hydatid fluid.

关键词

Keywords

references

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