DFMG maintains plaque stability in ApoE<sup>-/-</sup> mouse model of atherosclerosis by modulating angiogenesis

Pingjuan BO; Rubo WANG; Fei ZENG; Liping XIANG; Xueping XIANG; Xiaohua FU; Yong ZHANG

doi:10.16016/j.1000-5404.201803026

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DFMG maintains plaque stability in ApoE^-/- mouse model of atherosclerosis by modulating angiogenesis

更新时间：2026-01-09

- DFMG maintains plaque stability in ApoE^-/- mouse model of atherosclerosis by modulating angiogenesis
- Journal of Third Military Medical University Vol. 40, Issue 17, Pages: 1554-1560(2018)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81370382);the National Natural Science Foundation of Hunan Province(14JJ2059)
- DOI：10.16016/j.1000-5404.201803026
  CLC：
- Received：04 March 2018，
  
  Published：15 September 2018
- 稿件说明：
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Pingjuan BO, Rubo WANG, Fei ZENG, et al. DFMG maintains plaque stability in ApoE^-/- mouse model of atherosclerosis by modulating angiogenesis[J]. Journal of Third Military Medical University, 2018, 40(17): 1554-1560.
DOI：

Pingjuan BO, Rubo WANG, Fei ZENG, et al. DFMG maintains plaque stability in ApoE^-/- mouse model of atherosclerosis by modulating angiogenesis[J]. Journal of Third Military Medical University, 2018, 40(17): 1554-1560. DOI： 10.16016/j.1000-5404.201803026.

摘要

Abstract

Objective

To investigate the effect of 7-difluoromethoxy-5

4′-dimethoxygenistein (DFMG) on the angiogenesis and plaque stability in the atherosclerosis model of the Apolipoprotein E knockout mice (ApoE

-/-

Methods

A total of 20 ApoE

-/-

mice were randomly divided into 4 groups (

=5): model group

solvent (DMSO) group

DFMG group

and lovastatin group. And 5 C57BL/6 mice were assigned to the blank control group. The bla

nk control group was fed with normal diet and other 4 groups were fed with high-fat diet to induce atherosclerosis model. DFMG of 10 mg/(kg·d) was given to the mice of the DFMG group

and lovastatin 5 mg/(kg·d) to those of the lovastatin group. After 16 weeks

lipid content in the mice serum was measured by automatic biochemical analyzer. The lipid deposition in the gross thoracic aorta was evaluated by oil red O staining. The plaque stability in the thoracic aorta was measured by Masson staining. The angiogenesis was measured by the immunohistochemical staining with VEGF and vWF antibodies. The protein levels of TLR4 in the thoracic aorta were detected by Western blotting.

Results

DFMG decreased the ratio of atherosclerotic plaque to intraluminal diameter (

0.05)

reduced the levels of plasma lipids

including LDL

VLDL

TG and CHOL (

0.05)

and attenuated the amount of lipid red stains in the thoracic aorta (

0.05)

increased collagen in the plaque (

0.05) and lowered the expression of VEGF

vWF and TLR4 in the thoracic aorta (

0.05).

Conclusion

DFMG may inhibit the angiogenesis and maintain the stability of atherosclerotic plaque in the atherosclerotic ApoE

-/-

mice.

关键词

Keywords

references

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⁰

DFMG maintains plaque stability in ApoE-/- mouse model of atherosclerosis by modulating angiogenesis

DOI：10.16016/j.1000-5404.201803026

摘要

Abstract

关键词

Keywords

references

DFMG maintains plaque stability in ApoE^-/- mouse model of atherosclerosis by modulating angiogenesis