Roles of ICAT in regulation of PPARγ transcriptional activity and phenotypic transition of human mesangial cells induced by high glucose

Yi WEI; Jiawei YU; Jun ZHANG; Wei ZENG; Bing FENG

doi:10.16016/j.1000-5404.201803011

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Roles of ICAT in regulation of PPARγ transcriptional activity and phenotypic transition of human mesangial cells induced by high glucose

更新时间：2026-01-09

- Roles of ICAT in regulation of PPARγ transcriptional activity and phenotypic transition of human mesangial cells induced by high glucose
- Journal of Third Military Medical University Vol. 40, Issue 17, Pages: 1542-1547(2018)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81370820);the National Natural Science Foundation for Young Scholars of China(81400737)
- DOI：10.16016/j.1000-5404.201803011
  CLC：
- Received：02 March 2018，
  
  Published：15 September 2018
- 稿件说明：
移动端阅览
Yi WEI, Jiawei YU, Jun ZHANG, et al. Roles of ICAT in regulation of PPARγ transcriptional activity and phenotypic transition of human mesangial cells induced by high glucose[J]. Journal of Third Military Medical University, 2018, 40(17): 1542-1547.
DOI：

Yi WEI, Jiawei YU, Jun ZHANG, et al. Roles of ICAT in regulation of PPARγ transcriptional activity and phenotypic transition of human mesangial cells induced by high glucose[J]. Journal of Third Military Medical University, 2018, 40(17): 1542-1547. DOI： 10.16016/j.1000-5404.201803011.

摘要

Abstract

Objective

To explore the roles of β-catenin-interacting protein 1 (ICAT) in regulating peroxisome proliferator-activated receptor gamma (PPARγ) transcriptional activity and inducing cell phenotypic transition in human mesangial cells (HMCs) cultured in high glucose (HG).

Methods

HMCs were cultured in either 5.5 (NC) or 30 (HG) mmol/L glucose medium. RT-PCR was used to detect the expression of PPARγ

ICAT and β-catenin in the cells. The protein levels of PPARγ

ICAT and β-catenin were detected by Western blotting. Cell phenotype transition of HMCs was detected by the expression levels of α-smooth muscular actin (α-SMA). And CCK-8 assay was used to measure cell proliferation.

Results

Compared with NC

HG induced the expression of α-SMA and promoted the proliferation of HMCs. Though HG showed no significant influence on PPARγ expression in HMCs (

0.05)

it lowered the transcriptional activity of PPARγ (

0.05)

inhibited the expression of ICAT but enhanced β-catenin. Over-expression of ICAT partially promoted the transcriptional activity of PPARγ (

0.05). Under normal glucose condition

knockdown of ICAT reduced the transcriptional activity of PPARγ (

0.01)

so was β-catenin knockdown (

0.01). However

under high glucose condition

over-expression of ICAT resulted in decreased expression of α-SMA and reduced proliferation of HMCs.

Conclusion

ICAT might be one of potential molecules in regulation of PPARγ transcriptional activity

and it also participates in mediating the cell phenotypic transition of HMCs induced by high glucose.

关键词

Keywords

references

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