Electrospinning membranes with different microscopic morphologies: hydrophilicity, degradation, and influence on osteoblast proliferation and differentiation

Yigong ZHANG; Qiongyue ZHANG; Huaying SHAO; Xue YANG; Dan ZHANG; Yihui MA; Xiaohong WU

doi:10.16016/j.1000-5404.201803003

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Electrospinning membranes with different microscopic morphologies: hydrophilicity, degradation, and influence on osteoblast proliferation and differentiation

Basic Medicine | 更新时间：2026-01-09

- Electrospinning membranes with different microscopic morphologies: hydrophilicity, degradation, and influence on osteoblast proliferation and differentiation
- Journal of Third Military Medical University Vol. 40, Issue 14, Pages: 1295-1300(2018)
- 作者机构：
  
  4000015 重庆，重庆医科大学附属口腔医院修复科，口腔疾病与生物医学重庆市重点实验室，重庆市高校市级口腔生物医学工程重点实验室
- 作者简介：
- 基金信息：
  
  the Project for Innovation Team Construction of Higher Education in Chongqing(CXTDG201602006);the Project of Key Labrotary of Oral Biological Engineering of Higher Education in Chongqing;the Project of Chongqing Municipal Science and Technology Commission(cstc2017jcyjAX0215);the Project of Sci-Tech Development Program of Chongqing Yuzhong District(20170117)
- DOI：10.16016/j.1000-5404.201803003
  CLC： R322.71;R329-33;R329.24
- Received：01 March 2018，
  
  Revised：2018-04-17，
  
  Published：30 July 2018
- 稿件说明：
移动端阅览
Yigong ZHANG, Qiongyue ZHANG, Huaying SHAO, et al. Electrospinning membranes with different microscopic morphologies: hydrophilicity, degradation, and influence on osteoblast proliferation and differentiation[J]. Journal of Third Military Medical University, 2018, 40(14): 1295-1300.
DOI：

Yigong ZHANG, Qiongyue ZHANG, Huaying SHAO, et al. Electrospinning membranes with different microscopic morphologies: hydrophilicity, degradation, and influence on osteoblast proliferation and differentiation[J]. Journal of Third Military Medical University, 2018, 40(14): 1295-1300. DOI： 10.16016/j.1000-5404.201803003.

摘要

目的

制备微观下不同形态的聚己内酯(polycaprolactone，PCL)电纺纳米纤维膜并比较其亲水性、降解速度和对成骨细胞增殖、分化的影响。

方法

调整电纺参数制备出不同形态的电纺纤维膜，用扫描电子显微镜及倒置相差显微镜观察其形态，并通过可视接触角测试系统测试不同膜的表面接触角比较其亲水性，测量失重率评估其降解速度，细胞增殖实验、ALP活性实验和qRT-PCR实验评估其对成骨细胞增殖和分化的影响。

结果

15%、10%、8%组PCL所制得的电纺纤维膜在电子扫描显微镜500倍和倒置相差显微镜100倍下分别呈现出纯丝状、含有少量串珠的丝状、大量串珠状。3组电纺纤维膜的亲水性和对成骨细胞增殖的影响无明显差异；降解速度：纯丝组＜少量串珠组＜大量串珠组；成骨细胞分化的程度：纯丝组＞少量串珠组＞大量串珠组。

结论

调节电纺参数可制备不同形态的电纺纤维膜，纯丝状的电纺纤维膜较串珠状电纺纤维膜降解慢且更有利于成骨细胞的分化。

Abstract

Objective

To prepare electrospinning nanofiber membranes with different microscopic morphologies and compare their hydrophilicity

degradation

and influences on the proliferation and differentiation of osteoblasts.

Methods

Different electrospinning nanofiber membranes were prepared by adjusting the parameters

and the microscopic morphologies of the membranes were observed using scanning electron microscope and inverted phase-contrast microscope. The hydrophilicity and degradation of these membranes and their influences on the proliferation and differentiation of osteoblasts were analyzed using a visual contact angle test system

weight loss test

cell proliferation assay

ALP activity assay and qRT-PCR.

Results

Under scanning electron microscope (×500) and inverted phase-contrast microscope (×100)

the electrospinning nanofiber membranes prepared with 15%

10%

and 8% PCL showed a filamentous morphology

containing a small amount of beads

and a predominantly bead-like morphology

respectively. The 3 membranes exhibited similar hydrophilicity with also similar influences on osteoblast proliferation. The bead-like membrane had the highest degradation rate

followed by the bead-containing filamentous membrane and then by the filamentous membrane. The degree of osteoblasts differentiation on the 3 membranes was lowered in the order filamentous membrane

bead-containing filamentous membrane and bead-like membrane.

Conclusion

Electrospinning membrane with different microscopic morphologies can be prepared by adjusting the electrospinning parameters. Compared with the bead-containing and bead-like electrospinning membranes

the filamentous membrane is characterized by a slower degradation rate and a better capacity to promote the differentiation of osteoblasts.

关键词

Keywords

references

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