Comparison of demineralization kinetic curves of 2 commonly used demineralizing solutions and their effects on collagen dissolution and degradation before dentin bonding

Tingting KOU; Yu XIONG; Yue CUI; Jiangwen CAI; Can ZHU; Jixiang ZHOU

doi:10.16016/j.1000-5404.201802122

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Comparison of demineralization kinetic curves of 2 commonly used demineralizing solutions and their effects on collagen dissolution and degradation before dentin bonding

Biomedical Engineering | 更新时间：2026-01-09

- Comparison of demineralization kinetic curves of 2 commonly used demineralizing solutions and their effects on collagen dissolution and degradation before dentin bonding
- Journal of Third Military Medical University Vol. 40, Issue 12, Pages: 1155-1160(2018)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院口腔科
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81671022)
- DOI：10.16016/j.1000-5404.201802122
  CLC： R313;R783.1;R988.2
- Received：22 February 2018，
  
  Revised：2018-03-27，
  
  Published：2018-01
- 稿件说明：
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Tingting KOU, Yu XIONG, Yue CUI, et al. Comparison of demineralization kinetic curves of 2 commonly used demineralizing solutions and their effects on collagen dissolution and degradation before dentin bonding[J]. Journal of Third Military Medical University, 2018, 40(12): 1155-1160.
DOI：

Tingting KOU, Yu XIONG, Yue CUI, et al. Comparison of demineralization kinetic curves of 2 commonly used demineralizing solutions and their effects on collagen dissolution and degradation before dentin bonding[J]. Journal of Third Military Medical University, 2018, 40(12): 1155-1160. DOI： 10.16016/j.1000-5404.201802122.

摘要

目的

比较37%磷酸和17% EDTA对人牙本质的脱矿效率，并评价不同脱矿时间对人牙本质中Ⅰ型胶原基质降解速率的影响。

方法

制取等量人牙本质粉，分别以37%磷酸(Pi)与17% EDTA脱矿处理0 s(对照组)、10 s、15 s、30 s、60 s、120 s、72 h后离心荡洗。检测计算两种脱矿液的脱矿效率及胶原溶脱率并绘制动力学曲线。1 g/L Ⅰ型胶原酶溶液300 μL酶解样本沉淀，于1、7 d用羟脯胺酸(HYP)试剂盒测定并绘制胶原降解变化趋势图。利用Pearson相关系数分析脱矿总量与胶原溶脱、酶解1 d胶原降解量、酶解7 d胶原降解量及增量之间的相关性。

结果

磷酸组15 s即脱矿显著，胶原溶脱30 s明显增高(

0.05)，胶原降解量各时间点差异无统计学意义，降解增量15 s开始降低(

0.05)，脱矿量与胶原溶脱量及降解增量呈显著相关(

=0.864，

=-0.713，

0.05)。EDTA组30 s明显脱矿，胶原溶脱较少，且120s内各时间点差异无统计学意义，胶原降解量30 s达峰值，随后各时间点胶原降解量明显降低(

0.05)，降解增量60s开始降低(

0.05)，脱矿量与胶原溶脱量呈显著相关(

=0.894，

0.05)。

结论

磷酸脱矿效率迅速、高效，胶原溶脱率较高，稳定性较好；EDTA脱矿效率缓慢、持续，胶原溶脱率较低，稳定性尚可。临床进行牙本质粘结前表面处理牙本质时，以37%磷酸酸蚀10~15 s或17% EDTA表面处理60~90 s为较合理。

Abstract

Objective

To compare the demineralization efficiency of 37% phosphoric acid and 17% EDTA in human dentin and evaluate the effect of different etching time on the degradation rate of type Ⅰ collagen matrix in human dentin.

Methods

Equal amounts of human dentin powder were demineralized with 37% phosphoric acid and 17% EDTA for 0 s (control group)

60 and 120 s

or 72 h. After centrifugation and washing

the samples were analyzed for concentrations of Ca

and P

in the washing solution using an automatic biochemical analyzer. The demineralization curves were drawn and the demineralization efficiency of the 2 demineralization solutions was compared. The precipitates in each group were enzymatically hydrolyzed on day 1 and day 7 with 300 μL type Ⅰ collagenase solution (1 g/L). Pearson correlation coefficient was used to analyze the correlation among the total demineralization

collagen dissolution and collagen degradation.

Results

Treatment with 37% phosphoric acid solution for 15 s caused obvious demineralization in the dentin samples

and collagen dissolution increased significantly at 30 s (

0.05); collagen degradation levels did not show significant changes over time

and the increment of collagen degradation began to decrease significantly at 15 s (

0.05). The amount pf demineralization was strongly correlated with collagen dissolution (

=0.864

0.05) and degradation increment (

=-0.713

0.05)

. Treatment with 17% EDTA for 30 s resulted in obvious demineralization of dentin but less obvious collagen dissolution

which showed no significant changes within 120 s of treatment (

0.05); The peak level of collagen degradation occurred at 30 s followed by significant and progressive decreases (

0.05)

and the degradation increment began to decrease significantly at 60 s (

0.05). The amount of demineralization was strongly correlated with collagen dissolution (

=0.894

0.05).

Conclusion

Phosphoric acid results in rapid and efficient demineralization of dentin with a high collagen dissolution rate

and the dentin collagen matrix has a good stability after demineralization. EDTA has a relatively low efficiency of demineralization and collagen dissolution

and the stability of dentin collagen matrix after demineralization is acceptable. Clinically

both 37% phosphoric acid with an etching time of 10~15 s and 17% EDTA acid with an etching time of 60~90 s can be optimal for dentin treatment before dentin bonding.

关键词

Keywords

references

赵三军, 陈吉华.酸蚀时间对牙本质粘接界面纳米渗漏和粘接强度的影响[J].实用口腔医学杂志, 2009, 25(1): 36-40. DOI: 10.3969/j.issn.1001-3733.2009.01.08.

ZHAO S J, CHEN J H. Effects of acid etching time on nanoleakage and microtensile bond strength of the adhesive-dentin bond[J]. J Pract Stomatol, 2009, 25(1): 36-40. DOI: 10.3969/j.issn.1001-3733.2009.01.08.

裴丹丹, 黄雪清, 黄翠.牙本质混合层降解及其与牙本质粘接耐久性的关系[J].中华口腔医学杂志, 2011, 46(1): 58-60. DOI: 10.3760/cma.j.issn.1002-0098.2011.01.018.

PEI D D, HUANG X Q, HUANG C.Association of the degradation of the hybrid layer and the durability of dentin adhesion[J]. Chin J Stomatol, 2011, 46(1): 58-60. DOI: 10.3760/cma.j.issn.1002-0098.2011.01.018.

何恩宝, 王彦, 梁澜晨, 等.聚乙烯磷酸对牙本质基质金属蛋白酶活性的影响[J].中华口腔医学研究杂志(电子版), 2013, 7(4): 288-292. DOI: 10.3877/cma.j.issn.1674-1366.2013.04.006.

HE E B, WANG Y, LIANG L C, et al . The influence of polyvinylphosphonic acid on matrix metalloproteinases activities in dentin[J ] . Chin J Stomatol Res(Electron Ed), 2013, 7(4): 288-292. DOI: 10.3877/cma.j.issn.1674-1366.2013.04.006.

周恬, 朱梓园, 张保卫.牙本质内基质金属蛋白酶的测定及其对牙本质胶原降解作用的研究[J].口腔医学, 2012, 32(1): 25-28.

ZHOU T, ZHU Z Y, ZHANG B W. Study of measurement of matrix metalloproteinases in crown and root dentin and their effects on degradation of collagen fibers[J]. Stomatology, 2012, 32(1): 25-28.

SAURO S, TOLEDANO M, AGUILERA F S.Resin-dentin bonds to EDTA-treated vs. acid-etched dentin using ethanol wet-bonding. Part Ⅱ: Effects of mechanical cycling load on microtensile bond strengths[J]. Dent Mater, 2011, 27(6): 563-572. DOI: 10.1016/j.dental.2011.02.010.

OSORIO R, ERHARDT M C, PIMENTA L A, et al . EDTA treatment improves resin-dentin bonds' resistance to degradation[J ] . J Dent Res, 2005, 84(8): 736-740. DOI: 10.1177/154405910508400810.

TJÄDERHANE L, NASCIMENTO F D, BRESCHI L, et al .Optimizing dentin bond durability: control of collagen degradation by matrix metalloproteinases and cysteine cathepsins[J ] . Dent Mater, 2013, 29(1): 116-135. DOI: 10.1016/j.dental.2012.08.004.

HENCH L L.An Introduction to Bioceramics[M]. 2nd Edition. Singapore: World Scientific Publishing, 2013: 150-151.

KUMAR Y, LOHAR J, BHAT S, et al .Comparative evaluation of demineralization of radicular dentin with 17% EDTA and MTAD at different time intervals——An in-vitro study[J ] . Int Soc Prev Community Dent, 2016, 6(1): 44-48. DOI: 10.4103/2231-0762.175412..

MISHRA V K, BHATTACHARJEE B N, KUMAR D, et al .Effect of a chelating agent at different pH on the spectroscopic and structural properties of microwave derived hydroxyapatite nanoparticles: a bone mimetic material[J ] . New J Chemistry, 2016, 40(6): 5432-5441. DOI: 10.1039/C5NJ03322E.

HEDENBJÖRK-LAGER A, HAMBERG K, PÄÄKKÖNEN V, et al . Collagen degradation and preservation of MMP-8 activity in human dentine matrix after demineralization[J ] . Arch Oral Biol, 2016, 68: 66-72. DOI: 10.1016/j.archoralbio.2016.04.003.

许强建, 李全利, 陈佳龙, 等.多巴胺对基质金属蛋白酶活性及牙本质胶原纤维降解的影响[J].中华口腔医学杂志, 2015, 50(3): 186-189. DOI: 10.3760/cma.j.issn.1002-0098.2015.03.015.

XU Q J, LI Q L, CHEN J L, et al . Effect of dopamine on the activity of matrix metalloproteinases and degradation of dentin collagen[J ] . Chin J Stomatol, 2015, 50(3): 186-189.

SAI K, SHIMAMURA Y, TAKAMIZAWA T, et al . Influence of degradation conditions on dentin bonding durability of three universal adhesives[J ] . J Dent, 2016, 54: 56-61. DOI: 10.1016/j.jdent.2016.09.004.

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