Capsaicin enhances cisplatin sensitivity of osteosarcoma by inhibiting expression and nuclear-translocation of inhibitor of differentiation 1

Chang YU; Yang WANG; Mei XIONG; Zhengxue QUAN

doi:10.16016/j.1000-5404.201802097

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Capsaicin enhances cisplatin sensitivity of osteosarcoma by inhibiting expression and nuclear-translocation of inhibitor of differentiation 1

更新时间：2026-01-09

- Capsaicin enhances cisplatin sensitivity of osteosarcoma by inhibiting expression and nuclear-translocation of inhibitor of differentiation 1
- Journal of Third Military Medical University Vol. 40, Issue 16, Pages: 1492-1499(2018)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation(81272039);the Key Project of Chongqing Manicipal Science and Technology Committee(CSTC2017jcyjBX0017)
- DOI：10.16016/j.1000-5404.201802097
  CLC：
- Received：17 February 2018，
  
  Published：30 August 2018
- 稿件说明：
移动端阅览
Chang YU, Yang WANG, Mei XIONG, et al. Capsaicin enhances cisplatin sensitivity of osteosarcoma by inhibiting expression and nuclear-translocation of inhibitor of differentiation 1[J]. Journal of Third Military Medical University, 2018, 40(16): 1492-1499.
DOI：

Chang YU, Yang WANG, Mei XIONG, et al. Capsaicin enhances cisplatin sensitivity of osteosarcoma by inhibiting expression and nuclear-translocation of inhibitor of differentiation 1[J]. Journal of Third Military Medical University, 2018, 40(16): 1492-1499. DOI： 10.16016/j.1000-5404.201802097.

摘要

Abstract

Objective

To investigate the expression of inhibitor of differentiation 1 (Id1) in osteosarcoma tissues and evaluate the effect of capsaicin to enhance the chemosensitivity of osteosarcoma to cisplatin.

Methods

Immunohistochemistry was used to investigate Id1 expression levels in 12 osteosacroma tissue specimens and bone tissues from 12 patients with osteoarthritis.Three osteosarcoma cell lines (MG63

HOS and 143B) were treated with cisplatin and capsaicin

alone or in combination

and the expression levels of Id1

p-p65 and p-Smad1/5 in the cells were detected with Western blotting; immunofluorescence assay was used to investigate the location of Id1

and flow cytometry was used to analyze cell apoptosis in the treated cells.

Results

Osteosarcoma tissues expressed high levels of Id1 compared with osteoarthritis tissues.In the osteosarcoma cell lines

cisplain treatment induced an obvious up-regulation of Id1 expression (

0.05) and nuclear translocation of Id1

while treatment with capsaicin significantly reduced Id1 expression by suppressing p65 and Smad1/5 (

0.05).In cells treated with both capsaicin and cisplatin

capsaicin obviously reduced cisplatin-induced nuclear translocation of Id1 (

0.05) and significantly enhanced cisplatin-mediated cell apoptosis (

0.05).

Conclusion

Capsaicin can inhibit cisplatin-induced up-regulation and nuclear-translocation of Id1 and enhance cisplatin-mediated osteosarcoma cell apoptosis

and may thus serve as a potential agent for adjuvant chemotherapy of osteosarcoma.

关键词

Keywords

references

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