Low-frequency and low-intensity ultrasound suppresses activity of <italic style="font-style: italic">Mycobacterium smegmatis</italic> in macrophages

Shuang XIE; Min YANG; Yonghong DU; Dairong LI

doi:10.16016/j.1000-5404.201802075

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Low-frequency and low-intensity ultrasound suppresses activity of Mycobacterium smegmatis in macrophages

更新时间：2026-01-09

- Low-frequency and low-intensity ultrasound suppresses activity of Mycobacterium smegmatis in macrophages
- Journal of Third Military Medical University Vol. 40, Issue 16, Pages: 1476-1482(2018)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Project of Basic Science and Frontier Technology Research of Chongqing(CSCT2016jcyjA0098);the Project of Social Undertakings and People's Livelihood Security of Chongqing(CSCT2016shmszx130029)
- DOI：10.16016/j.1000-5404.201802075
  CLC：
- Received：15 February 2018，
  
  Published：30 August 2018
- 稿件说明：
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Shuang XIE, Min YANG, Yonghong DU, et al. Low-frequency and low-intensity ultrasound suppresses activity of Mycobacterium smegmatis in macrophages[J]. Journal of Third Military Medical University, 2018, 40(16): 1476-1482.
DOI：

Shuang XIE, Min YANG, Yonghong DU, et al. Low-frequency and low-intensity ultrasound suppresses activity of Mycobacterium smegmatis in macrophages[J]. Journal of Third Military Medical University, 2018, 40(16): 1476-1482. DOI： 10.16016/j.1000-5404.201802075.

摘要

Abstract

Objective

To investigate the effect of low-frequency and low-intensity ultrasound (LFLIU) in inducing damages of

Mycobacterium smegmatis

(

M.smegmatis

) in macrophages and explore the mechanism.

Methods

Murine macrophage RAW264.7 cells were exposed to ultrasound irradiation for 5

10 or 15 min

and the changes in the cell viability were analyzed with MTT assay.The apoptotic and necrosis rates of RAW264.7 cells after ultrasonic irradiation and after phagocytosis of

M.smegmatis

were assessed using flow cytometry; the activity of

M.smegmatis

in RAW264.7 cells following the exposure was evaluated with plate colony counting.

Laser scanning confocal microscopy was used to observe the production of reactive oxygen species (ROS) in RAW264.7 cells after ultrasound exposure.

Results

Exposure of RAW264.7 cells to a ultrasonic intensity of 0.14 W/cm

for 5 min and 10 min resulted in cell survival rates of (116.37±9.54)% and (97.14±9.26)%

respectively

suggesting no significant impact of LFLIU exposure on the cell viability.The apoptotic rate of RAW264.7 cells increased significantly after irradiation with 0.14 W/cm

ultrasound for 5 min compared with the control cells (

0.05).The results of plate colony counting suggested that the viability of

M.smegmatis

in RAW264.7 cells was significantly lowered after exposure to a ultrasound intensity of 0.14 W/cm

for 5 and 10 min.Confocal laser scanning microscopy revealed obvious ROS production in RAW264.7 cells in response to 0.14 W/cm

ultrasound exposure.

Conclusion

Exposure to LFLIU induces apoptosis and ROS production in RAW264.7 cells and causes decreased activity of

M.smegmatis

within the cells.

关键词

Keywords

references

RUSSELL D G Who puts the tubercle in tuberculosis? Nat Rev Microbiol 2007 5 1 39 47 10.1038/nrmicro1538 RUSSELL D G. Who puts the tubercle in tuberculosis?[J]. Nat Rev Microbiol, 2007, 5(1): 39-47. DOI: 10.1038/nrmicro1538.

JOHNSON E E SRIKANTH C V SANDGREN A Siderocalin inhibits the intracellular replication of Mycobacterium tuberculosis in macrophages FEMS Immunol Med Microbiol 2010 58 1 138 145 10.1111/j.1574-695X.2009.00622.x JOHNSON E E, SRIKANTH C V, SANDGREN A, et al . Siderocalin inhibits the intracellular replication of Mycobacterium tuberculosis in macrophages[J ] . FEMS Immunol Med Microbiol, 2010, 58(1): 138-145. DOI: 10.1111/j.1574-695X.2009.00622.x.

YU H CHEN S CAO P Synergistic bactericidal effects and mechanisms of low intensity ultrasound and antibiotics against bacteria: a review Ultrason Sonochem 2012 19 3 377 382 10.1016/j.ultsonch.2011.11.010 YU H, CHEN S, CAO P. Synergistic bactericidal effects and mechanisms of low intensity ultrasound and antibiotics against bacteria: a review[J]. Ultrason Sonochem, 2012, 19(3): 377-382. DOI: 10.1016/j.ultsonch.2011.11.010.

DONG Y SU H JIANG H Experimental study on the influence of low-frequency and low-intensity ultrasound on the permeability of the Mycobacterium smegmatis cytoderm and potentiation with levofloxacin Ultrason Sonochem 2017 37 1 8 10.1016/j.ultsonch.2016.12.024 DONG Y, SU H, JIANG H, et al . Experimental study on the influence of low-frequency and low-intensity ultrasound on the permeability of the Mycobacterium smegmatis cytoderm and potentiation with levofloxacin[J ] . Ultrason Sonochem, 2017, 37: 1-8. DOI: 10.1016/j.ultsonch.2016.12.024.

SU H LI Z DONG Y Damage effects on bacille calmette-guérin by low-frequency, low-intensity ultrasound: a pilot study J Ultrasound Med 2016 35 3 581 587 10.7863/ultra.14.11056 SU H, LI Z, DONG Y, et al . Damage effects on bacille calmette-guérin by low-frequency, low-intensity ultrasound: a pilot study[J ] . J Ultrasound Med, 2016, 35(3): 581-587. DOI: 10.7863/ultra.14.11056.

TUDELA I SáEZ V ESCLAPEZ M D Simulation of the spatial distribution of the acoustic pressure in sonochemical reactors with numerical methods: a review Ultrason Sonochem 2014 21 3 909 919 10.1016/j.ultsonch.2013.11.012 TUDELA I, SáEZ V, ESCLAPEZ M D, et al .Simulation of the spatial distribution of the acoustic pressure in sonochemical reactors with numerical methods: a review[J ] . Ultrason Sonochem, 2014, 21(3): 909-919. DOI: 10.1016/j.ultsonch.2013.11.012.

REYRAT J M KAHN D Mycobacterium smegmatis: an absurd model for tuberculosis Trends Microbiol 2001 9 10 472 474 10.1016/S0966-842X(01)02169-2 REYRAT J M, KAHN D. Mycobacterium smegmatis: an absurd model for tuberculosis[J]. Trends Microbiol, 2001, 9(10): 472-474. DOI: 10.1016/S0966-842X(01)02169-2.

SOTGIU G SULIS G MATTEELLI A Tuberculosis-a world health organization perspective Microbiol Spectr 2017 5 1 1 18 10.1128/microbiolspec SOTGIU G, SULIS G, MATTEELLI A. Tuberculosis-a world health organization perspective[J]. Microbiol Spectr, 2017, 5(1): 1-18. DOI: 10.1128/microbiolspec.

GENGENBACHER M KAUFMANN S H Mycobacterium tuberculosis: success through dormancy FEMS Microbiol Rev 2012 36 3 514 532 10.1111/j.1574-6976.2012.00331.x GENGENBACHER M, KAUFMANN S H. Mycobacterium tuberculosis: success through dormancy[J]. FEMS Microbiol Rev, 2012, 36(3): 514-532.DOI: 10.1111/j.1574-6976.2012.00331.x.

WU X JOYCE E M MASON T J Evaluation of the mechanisms of the effect of ultrasound on Microcystis aeruginosa at different ultrasonic frequencies Water Res 2012 46 9 2851 2858 10.1016/j.watres.2012.02.019 WU X, JOYCE E M, MASON T J. Evaluation of the mechanisms of the effect of ultrasound on Microcystis aeruginosa at different ultrasonic frequencies[J]. Water Res, 2012, 46(9): 2851-2858. DOI: 10.1016/j.watres.2012.02.019.

ALEXANDROV B S RASMUSSEN K Ø BISHOP A R Non-thermal effects of terahertz radiation on gene expression in mouse stem cells Biomed Opt Express 2011 2 9 2679 2689 10.1364/BOE.2.002679 ALEXANDROV B S, RASMUSSEN K Ø, BISHOP A R, et al . Non-thermal effects of terahertz radiation on gene expression in mouse stem cells[J ] . Biomed Opt Express, 2011, 2(9): 2679-2689. DOI: 10.1364/BOE.2.002679.

BEDARD K KRAUSE K H The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology Physiol Rev 2007 87 1 245 313 10.1152/physrev.00044.2005 BEDARD K, KRAUSE K H. The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology[J]. Physiol Rev, 2007, 87(1): 245-313. DOI: 10.1152/physrev.00044.2005.

ISLAM M A PRÖLL M HÖLKER M Alveolar macrophage phagocytic activity is enhanced with LPS priming, and combined stimulation of LPS and lipoteichoic acid synergistically induce pro-inflammatory cytokines in pigs Innate Immun 2013 19 6 631 643 10.1177/1753425913477166 ISLAM M A, PRÖLL M, HÖLKER M, et al . Alveolar macrophage phagocytic activity is enhanced with LPS priming, and combined stimulation of LPS and lipoteichoic acid synergistically induce pro-inflammatory cytokines in pigs[J ] . Innate Immun, 2013, 19(6): 631-643. DOI: 10.1177/1753425913477166.

LEE H Z LIN C J YANG W H Aloe-emodin induced DNA damage through generation of reactive oxygen species in human lung carcinoma cells Cancer Lett 2006 239 1 55 63 10.1016/j.canlet.2005.07.036 LEE H Z, LIN C J, YANG W H , et al .Aloe-emodin induced DNA damage through generation of reactive oxygen species in human lung carcinoma cells[J ] . Cancer Lett, 2006, 239(1): 55-63. DOI: 10.1016/j.canlet.2005.07.036.

HUANG S K ZHANG Q QIU Z Mechanistic impact of outdoor air pollution on asthma and allergic diseases J Thorac Dis 2015 7 1 23 33 10.3978/j.issn.2072-1439.2014.12.13 HUANG S K, ZHANG Q, QIU Z, et al . Mechanistic impact of outdoor air pollution on asthma and allergic diseases[J ] . J Thorac Dis, 2015, 7(1): 23-33. DOI: 10.3978/j.issn.2072-1439.2014.12.13.

WEISS G SCHAIBLE U E Macrophage defense mechanisms against intracellular bacteria Immunol Rev 2015 264 1 182 203 10.1111/imr.12266 WEISS G, SCHAIBLE U E. Macrophage defense mechanisms against intracellular bacteria[J]. Immunol Rev, 2015, 264(1): 182-203. DOI: 10.1111/imr.12266.

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⁰

Low-frequency and low-intensity ultrasound suppresses activity of Mycobacterium smegmatis in macrophages

DOI：10.16016/j.1000-5404.201802075

摘要

Abstract

关键词

Keywords

references