Expression and significance of serum content of vitamin A and intestinal expression of retinoic acid receptor α in children with congenital anorectal malformation

Zhili WANG; Chengchao GU; Quan WANG; Jing SUN; Jinping HOU; Wei LIU; Xiaoqing LI; Yang BI; Yi WANG

doi:10.16016/j.1000-5404.201802071

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Expression and significance of serum content of vitamin A and intestinal expression of retinoic acid receptor α in children with congenital anorectal malformation

更新时间：2026-01-09

- Expression and significance of serum content of vitamin A and intestinal expression of retinoic acid receptor α in children with congenital anorectal malformation
- Journal of Third Military Medical University Vol. 40, Issue 17, Pages: 1584-1589(2018)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Key Project of National Clinical Keydiscipline Construction Programs of China(国卫办医函〔2013〕544);the Key Project of Medical Scientific Research of Chongqing Municipal Health and Family Planning Commission(20141010)
- DOI：10.16016/j.1000-5404.201802071
  CLC：
- Received：09 February 2018，
  
  Published：15 September 2018
- 稿件说明：
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Zhili WANG, Chengchao GU, Quan WANG, et al. Expression and significance of serum content of vitamin A and intestinal expression of retinoic acid receptor α in children with congenital anorectal malformation[J]. Journal of Third Military Medical University, 2018, 40(17): 1584-1589.
DOI：

Zhili WANG, Chengchao GU, Quan WANG, et al. Expression and significance of serum content of vitamin A and intestinal expression of retinoic acid receptor α in children with congenital anorectal malformation[J]. Journal of Third Military Medical University, 2018, 40(17): 1584-1589. DOI： 10.16016/j.1000-5404.201802071.

摘要

Abstract

Objective

To investigate the serum content of vitamin A (VA) and the intestinal expression of retinoic acid receptor α (RARα) in the children with congenital anorectal malformation (ARM)

and explore the possible association of them with abnormalities of ARM enteric nervous system (ENS).

Methods

A total of 32 freshly-diagnosed ARM neonates who undergoing elective staged surgical treatment in our hospital from February 2015 to February 2017 were recruited in this study. High performance liquid chromatography was used to detect the serum VA content in the 32 ARM neonates and 30 neonates (as control) without alimentary tract malformations. RT-PCR was employed to detect the mRNA expression of RARα in intestinal specimens at the end of rectum and stoma from 30 ARM neonates and 4 neonates (as control) without alimentary tract malformations. According to the results of pathological study on the intestinal tissues at the end of rectum

the tissues were divided into normal and abnormal ganglion cells groups (

=6 for each group). Then immunohistochemical assay and Western blotting were adopted to detect the expression of RARα protein in the rectum specimens.

Results

The serum content of VA was significantly lower in the ARM neonates than the control neonates (0.40±0.14

0.54±0.10 μmol/L

0.01). The expression level of RARα mRNA was significantly lower in the rectum of ARM (0.78±0.40) than in the

colon of stoma (1.28±0.72

0.01) and the rectum of control (1.12±0.14

0.01)

but there was no difference in the level between the colon of stoma and rectum of control (

0.05). RARα were expressed in the nucleus of ganglion cells and nerve fibers in the rectum. The protein level of RARα was significantly lower in the abnormal ganglion cells group than the normal ganglion cells group (0.77±0.08

1.03±0.06

0.01).

Conclusion

ARM neonates have lower serum VA content and abnormal intestinal expression of RARα.

关键词

Keywords

references

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