Genomic analysis of vaginal microbiome community in female early rheumatoid arthritis patients

Caixia HUI; Juan ZHANG; Benhua ZENG; Bing ZHONG; Ping WAN; Li YANG; Like WU; Hong WEI; Yongfei FANG

doi:10.16016/j.1000-5404.201802065

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Genomic analysis of vaginal microbiome community in female early rheumatoid arthritis patients

更新时间：2026-01-09

- Genomic analysis of vaginal microbiome community in female early rheumatoid arthritis patients
- Journal of Third Military Medical University Vol. 40, Issue 11, Pages: 1005-1010(2018)
- 作者机构：
- 作者简介：
- 基金信息：
  
  Supported by the General Program of National Natural Science Foundation of China(81072921)
- DOI：10.16016/j.1000-5404.201802065
  CLC：
- Received：07 February 2018，
  
  Published：15 June 2018
- 稿件说明：
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Caixia HUI, Juan ZHANG, Benhua ZENG, et al. Genomic analysis of vaginal microbiome community in female early rheumatoid arthritis patients[J]. Journal of Third Military Medical University, 2018, 40(11): 1005-1010.
DOI：

Caixia HUI, Juan ZHANG, Benhua ZENG, et al. Genomic analysis of vaginal microbiome community in female early rheumatoid arthritis patients[J]. Journal of Third Military Medical University, 2018, 40(11): 1005-1010. DOI： 10.16016/j.1000-5404.201802065.

摘要

Abstract

Objective

To analyze the differences of vaginal microbiome between the patients with early rheumatoid arthritis (RA) and healthy reproductive-age women from the overall perspective of bacteria.

Methods

A total of 24 vaginal swabs were collected from 12 early RA patients and 12 healthy women (control). The bacterial genomic DNA of all obtained samples was extracted for amplifying the 16S rRNA V3 and V4 hypervariable region by PCR and pyrosequencing by Illumina. The abundance and composition of vaginal microbiota were analyzed by Uparse and LEfSe software.

Results

The Alpha diversity was significantly increased in the bacterial strains from the early RA patients (P=0.04). Lactobacillus was the predominant genus in healthy women (accounting for 84.98%). The amount of Lactobacillus was decreased (11.01%) but that of Gardnerella increased (43.16%) significantly in the RA group. Moreover

the relative abundances of Bifidobacterium (9.94%)

Streptococcus (8.05%) and Atopobium (7.86%) were increased in different degrees. The results of principal coordinate analysis (PCoA) and hierarchical cluster analysis showed that the structure of vaginal flora in RA group had high similarity

and the vaginal microflora structure of the healthy control group could be distinctly distinguished. LEfSe analysis found that there were signific

ant differences in the abundance of Lactobacillus

Gardiner

bifidobacteria and Singularities at the genus level (

=0.017

0.046

0.018

0.007).

Conclusion

A correlation is found between vaginal dysbacteriosis and RA. The diversity of vaginal microbiome is increased in the RA patients

with significantly decreased relative abundance of Lactobacillus

while increased abundance of Gardnerella.

关键词

Keywords

references

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