Therapeutic effect of camostat in a mouse model of acute pancreatitis

Yao YAO; Lu ZHUANG; Kun LIN; Baoan JI; Haojie HUANG; Zhaoshen LI

doi:10.16016/j.1000-5404.201801230

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Therapeutic effect of camostat in a mouse model of acute pancreatitis

Basic Medicine | 更新时间：2026-01-09

- Therapeutic effect of camostat in a mouse model of acute pancreatitis
- Journal of Third Military Medical University Vol. 40, Issue 14, Pages: 1291-1294(2018)
- 作者机构：
  
  1.200433 上海，海军军医大学(第二军医大学)附属长海医院消化内科
  2.32224美国佛罗里达州，美国Mayo Clinic肿瘤生物系
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201801230
  CLC： R-332; R576.05; R977.3
- Received：29 January 2018，
  
  Revised：2018-03-17，
  
  Published：30 July 2018
- 稿件说明：
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Yao YAO, Lu ZHUANG, Kun LIN, et al. Therapeutic effect of camostat in a mouse model of acute pancreatitis[J]. Journal of Third Military Medical University, 2018, 40(14): 1291-1294.
DOI：

Yao YAO, Lu ZHUANG, Kun LIN, et al. Therapeutic effect of camostat in a mouse model of acute pancreatitis[J]. Journal of Third Military Medical University, 2018, 40(14): 1291-1294. DOI： 10.16016/j.1000-5404.201801230.

摘要

目的

探究胰蛋白酶制剂卡莫司他对雨蛙素诱导的急性胰腺炎的作用。

方法

用雨蛙素腹腔注射法诱导C57BL/6J小鼠建立急性胰腺炎模型，以卡莫司他灌胃法为治疗组，生理盐水灌胃法为对照组。通过观察小鼠行为学的变化和检测各组小鼠血清淀粉酶水平，比较胰腺质量/体质量、胰腺组织病理变化等指标，分析炎症的不同损伤程度及变化。

结果

治疗组小鼠的胰腺质量/体质量和血清淀粉酶水平均低于非治疗组；非治疗组C57BL/6J小鼠注射雨蛙素后有急性胰腺炎的典型病理组织学改变，而治疗组小鼠则炎症明显改善，水肿及坏死均减轻，炎症细胞浸润减少。治疗组和对照组之间的胰腺质量/体质量和血清淀粉酶水平比较差异均没有统计学意义。

结论

卡莫司他可通过抑制胰腺蛋白酶活性减轻急性胰腺炎的炎症严重程度。

Abstract

Objective

To explore the therapeutic effect of camostat

a trypsinogen inhibitor

on cerulein-induced acute pancreatitis in mice.

Methods

A mouse model of acute pancreatitis was established by intraperitoneal injection of cerulein and treated with camostat (camostat treatment group) or normal saline (model group) administered by oral gavage

with mice receiving both intraperitoneal injection and oral gavage of saline serving as the control group. The changes in the behaviors

serum amylase levels

pancreas/body weight ratio and pancreas histology of the mice were observed after the treatment to evaluate the changes in the inflammation.

Results

Compared with the mice in the model group

the mice in camostat treatment group showed significantly lowered pancreas/body weight ratio and serum amylase level with obviously alleviated pancreatic edema

necrosis and inflammatory cell infiltration. No significant difference was found in pancreas/body ratio or serum amylase between camostat treatment group and the control group.

Conclusion

In the mouse model of cerulein-induced acute pancreatitis

camostat treatment alleviates the inflammation by inhibiting trypsin activity

which highlights the role of trypsin in the process of acute pancreatitis.

关键词

Keywords

references

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