Effects of aspirin on proliferation and apoptosis of mouse glioma stem cells <italic style="font-style: italic">in vitro</italic>

Dewei ZOU; Junwei WANG; Pan WANG; Bin LIAO; Jinyu PAN; Chengren LI; Nan WU

doi:10.16016/j.1000-5404.201801221

您当前的位置：

首页 >

文章列表页 >

Effects of aspirin on proliferation and apoptosis of mouse glioma stem cells in vitro

更新时间：2026-01-09

- Effects of aspirin on proliferation and apoptosis of mouse glioma stem cells in vitro
- Journal of Third Military Medical University Vol. 40, Issue 16, Pages: 1456-1460(2018)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81672493);Project of Southwest Hospital(SWH2016JCYB-42)
- DOI：10.16016/j.1000-5404.201801221
  CLC：
- Received：25 January 2018，
  
  Published：30 August 2018
- 稿件说明：
移动端阅览
Dewei ZOU, Junwei WANG, Pan WANG, et al. Effects of aspirin on proliferation and apoptosis of mouse glioma stem cells in vitro[J]. Journal of Third Military Medical University, 2018, 40(16): 1456-1460.
DOI：

Dewei ZOU, Junwei WANG, Pan WANG, et al. Effects of aspirin on proliferation and apoptosis of mouse glioma stem cells in vitro[J]. Journal of Third Military Medical University, 2018, 40(16): 1456-1460. DOI： 10.16016/j.1000-5404.201801221.

摘要

Abstract

Objective

To study the effects of aspirin on proliferation and apoptosis of mouse glioma stem cells (GSCs).

Methods

In vitro

cultured GSCs isolated from mouse glioma cell line GL261 were treated with 2.5

5.0 and 10 mmol/L aspirin

with normal saline as the control.The pro

liferation of GSCs was assayed using CCK-8 kit

and the changes in cell cycle distribution and cell apoptosis were detected with flow cytometry and TUNEL assay

respectively; LDH kit was used to assess the cell damage after aspirin treatment.

Results

Compared with the control cells

the GSCs treated with different concentrations of aspirin showed dose- and time-dependent inhibition of cell proliferation (

0.05) and obviously reduced number and size of the tumor spheres (

0.05).Aspirin caused obvious cell cycle changes in GSCs

shown by a significantly decreased percentage of S phase cells and an increased percentage of G

phase cells (

0.05).Aspirin also induced obvious apoptosis of GSCs and significantly increased the release of cytosolic LDH (

0.05).

Conclusion

Aspirin inhibits the proliferation and promotes apoptosis of mouse GSCs

in vitro

关键词

Keywords

references

KONG B H MOON J H HUH Y M Prognostic value of glioma cancer stem cell isolation in survival of primary glioblastoma patients Stem Cells Int 2014 2014 838950 10.1155/2014/838950 KONG B H, MOON J H, HUH Y M, et al . Prognostic value of glioma cancer stem cell isolation in survival of primary glioblastoma patients[J ] . Stem Cells Int, 2014, 2014: 838950. DOI: 10.1155/2014/838950.

SINGH S K HAWKINS C CLARKE I D Identification of human brain tumour initiating cells Nature 2004 432 7015 396 401 10.1038/nature03128 SINGH S K, HAWKINS C, CLARKE I D, et al . Identification of human brain tumour initiating cells[J ] . Nature, 2004, 432(7015): 396-401. DOI: 10.1038/nature03128.

MITRUGNO A SYLMAN J L RIGG R A Carpe low-dose aspirin: the new anti-cancer face of an old anti- platelet drug Platelets 2017 21 1 6 10.1080/09537104.2017.1416076 MITRUGNO A, SYLMAN J L, RIGG R A, et al .Carpe low-dose aspirin: the new anti-cancer face of an old anti- platelet drug[J ] . Platelets, 2017, 21: 1-6. DOI: 10.1080/09537104. 2017.1416076.

HARRIS R E BEEBE-DON K J DOS S H Aspirin, ibuprofen, and other non-steroidal anti-inflammatory drugs in cancer prevention: a critical review of non-selective COX2 blockade(review) Oncol Rep 2005 13 4 559 583 https://www.ncbi.nlm.nih.gov/pubmed/15756426 HARRIS R E, BEEBE-DON K J, DOS S H, et al .Aspirin, ibuprofen, and other non-steroidal anti-inflammatory drugs in cancer prevention: a critical review of non-selective COX2 blockade(review)[J ] . Oncol Rep, 2005, 13(4): 559-583.

GAIST D GARCIA-RODRIGUEZ L A SORENSEN H T Use of low-dose aspirin and non-aspirin nonsteroidal anti-inflammatory drugs and risk of glioma: a case-control study Br J Cancer 2013 108 5 1189 1194 10.1038/bjc.2013.87 GAIST D, GARCIA-RODRIGUEZ L A, SORENSEN H T, et al . Use of low-dose aspirin and non-aspirin nonsteroidal anti-inflammatory drugs and risk of glioma: a case-control study[J ] . Br J Cancer, 2013, 108(5): 1189-1194. DOI: 10.1038/bjc.2013.87.

LAN F YUE X HAN L Antitumor effect of aspirin in glioblastoma cells by modulation of β-catenin/T-cell factor-mediated transcriptional activity J Neurosurg 2011 115 4 780 788 10.3171/2011.5.JNS113 LAN F, YUE X, HAN L, et al . Antitumor effect of aspirin in glioblastoma cells by modulation of β-catenin/T-cell factor-mediated transcriptional activity[J ] . J Neurosurg, 2011, 115(4): 780-788. DOI: 10.3171/2011.5.JNS113.

MAITY G DE A DAS A Aspirin blocks growth of breast tumor cells and tumor-initiating cells and induces reprogramming factors of mesenchymal to epithelial transition Lab Invest 2015 95 7 702 717 10.1038/labinvest.2015.49 MAITY G, DE A, DAS A, et al .Aspirin blocks growth of breast tumor cells and tumor-initiating cells and induces reprogramming factors of mesenchymal to epithelial transition[J ] .Lab Invest, 2015, 95(7): 702-717. DOI: 10.1038/labinvest.2015.49.

WANG Y HUANG N X LI H L Promoting oligodendroglial-oriented differentiation of glioma stem cell: a repurposing of quetiapine for the treatment of malignant glioma Oncotarget 2017 8 23 37511 3752 10.18632/oncotarget.16400 WANG Y, HUANG N X, LI H L, et al . Promoting oligodendroglial-oriented differentiation of glioma stem cell: a repurposing of quetiapine for the treatment of malignant glioma[J ] . Oncotarget, 2017, 8(23): 37511-3752. DOI: 10.18632/oncotarget.16400.

DIETRICH J DIAMOND E L KESARI S Glioma stem cell signaling: therapeutic opportunities and challenges Expert Rev Anticancer Ther 2010 10 5 709 722 10.1586/era.09.190 DIETRICH J, DIAMOND E L, KESARI S. Glioma stem cell signaling: therapeutic opportunities and challenges[J]. Expert Rev Anticancer Ther, 2010, 10(5): 709-722. DOI: 10.1586/era.09.190.

SIVAK-SEARS N R SCHWARTZBAUM J A MⅡKE R Case-control study of use of nonsteroidal anti-inflammatory drugs and glioblastoma multiforme Am J Epidemiol 2004 159 12 1131 1139 10.1093/aje/kwh153 SIVAK-SEARS N R, SCHWARTZBAUM J A, MⅡKE R, et al . Case-control study of use of nonsteroidal anti-inflammatory drugs and glioblastoma multiforme[J ] . Am J Epidemiol, 2004, 159(12): 1131-1139. DOI: 10.1093/aje/kwh153.

KIM S R BAEM K KIM J Y Aspirin in duces apoptosis through the blockade of IL-6-STAT3 signaling pathway in human glioblastoma A172 cells Biochem Biophys Res Commun 2009 387 2 342 347 10.1016/j.bbrc.2009.07.022 KIM S R, BAEM K, KIM J Y, et al . Aspirin in duces apoptosis through the blockade of IL-6-STAT3 signaling pathway in human glioblastoma A172 cells[J ] .Biochem Biophys Res Commun, 2009, 387(2): 342-347. DOI: 10.1016/j.bbrc.2009.07.022.

ZHANG Y LIU L FAN P Aspirin counteracts cancerstem cell features, desmoplasia and gemcitabine resistancein pancreatic cancer Oncotarget 2015 6 12 9999 10015 10.18632/oncotarget.3171 ZHANG Y, LIU L, FAN P, et al .Aspirin counteracts cancerstem cell features, desmoplasia and gemcitabine resistancein pancreatic cancer[J ] .Oncotarget, 2015, 6(12): 9999-10015. DOI: 10.18632/oncotarget.3171.

MOON C M KWON J H KIM J S Nonsteroidal anti-inflammatory drugs suppress cancer stem cells via inhibiting PTGS2(cyclooxygenase 2)and NOTCH/HES1 and activating PPARG in colorectal cancer Int J Cancer 2014 134 3 519 529 10.1002/ijc.28381 MOON C M, KWON J H, KIM J S, et al .Nonsteroidal anti-inflammatory drugs suppress cancer stem cells via inhibiting PTGS2(cyclooxygenase 2)and NOTCH/HES1 and activating PPARG in colorectal cancer[J ] . Int J Cancer, 2014, 134(3): 519-529. DOI: 10.1002/ijc.28381.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Analysis on advantages of autofluorescence technique in screening malignant of glioma stem cells

Value of MRI radiomics based on intratumoral and peritumoral heterogeneity in predicting spatial patterns of locally recurrent high-grade gliomas

Hypoxic transcriptional phenotype and cellular ultrastructural changes of tumor-associated macrophages in gliomas

Value of ambulatory electroencephalogram in evaluation of postoperative withdrawal of antiepileptic drugs in patients with glioma-related epilepsy

Application of different post-processing modes of dynamic contrast enhanced magnetic resonance imaging in glioma grading

Related Author

Bijia WANG

Zhenhua ZHOU

Kangning CHEN

Xuegang LI

WANG Hanwei

ZENG Linlan

ZHAO Mimi

LI Xuan

Related Institution

Department of Neurosurgery, First Affiliated Hospital, Army Medical University (Third Military Medical University)

Department of Neurology, First Affiliated Hospital, Army Medical University (Third Military Medical University)

Department of Radiology， Chongqing Clinical Research Centre of Imaging and Nuclear Medicine, Faculty of Biomedical Engineering and Imaging Medicine， Army Medical University （Third Military Medical University）

Department of Neurosurgery， Army Medical Center of PLA/Daping Hospital of Third Military Medical University， Chongqing, Faculty of Biomedical Engineering and Imaging Medicine， Army Medical University （Third Military Medical University）

Department of Digital Medicine, Faculty of Biomedical Engineering and Imaging Medicine， Army Medical University （Third Military Medical University）

⁰

Effects of aspirin on proliferation and apoptosis of mouse glioma stem cells in vitro

DOI：10.16016/j.1000-5404.201801221

摘要

Abstract

关键词

Keywords

references