Dynamic evaluation of inflammation activity in rheumatoid arthritis rabbits using contrast-enhanced ultrasound

Changjie WU; Yahui WANG; Xing HUA; Yanli GUO; Yongliang TENG; Xueting LIU

doi:10.16016/j.1000-5404.201801218

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Dynamic evaluation of inflammation activity in rheumatoid arthritis rabbits using contrast-enhanced ultrasound

更新时间：2026-01-09

- Dynamic evaluation of inflammation activity in rheumatoid arthritis rabbits using contrast-enhanced ultrasound
- Journal of Third Military Medical University Vol. 40, Issue 11, Pages: 984-990(2018)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81471677)
- DOI：10.16016/j.1000-5404.201801218
  CLC：
- Received：26 January 2018，
  
  Published：2018-06
- 稿件说明：
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Changjie WU, Yahui WANG, Xing HUA, et al. Dynamic evaluation of inflammation activity in rheumatoid arthritis rabbits using contrast-enhanced ultrasound[J]. Journal of Third Military Medical University, 2018, 40(11): 984-990.
DOI：

Changjie WU, Yahui WANG, Xing HUA, et al. Dynamic evaluation of inflammation activity in rheumatoid arthritis rabbits using contrast-enhanced ultrasound[J]. Journal of Third Military Medical University, 2018, 40(11): 984-990. DOI： 10.16016/j.1000-5404.201801218.

摘要

Abstract

Objective

To evaluate the value of contrast-enhanced ultrasound (CEUS) in the dynamic detection of inflammation activity in rabbit model of rheumatoid arthritis (RA).

Methods

A total of 24 rabbits (both sexes

6 months old

weighing 3.0~3.5 kg) were used to establish RA model

via

ovalbumin sensitization in 48 knee joints. The rabbits were divided into 4 groups

that is

control (pre-modeling)

M1 (1 week post induction)

M4 (4 weeks post induction) and M8 (8 weeks post induction) groups

with 12 knees in each group. Ultrasound examination including grey scale ultrasonography and power Doppler imaging (PDI) was performed in both femorotibial joints of each rabbit to measure the synovial thickness and semi-quantitatively score (0~3). CEUS was subsequently performed to evaluate the enhancement of synovium. Time-intensity curve (TIC) was drawn with automatic fitting

and the data including peak intensity (PI)

peak time (PT)

area under curve (AUC) and half time of descending (T1/2) were determined automatically. All the rats were killed

and the synovial tissues were collected for pathological examination. The synovial vascular bed area index was calculated in each group

and the results were compared. The correlations between ultrasonic data and pathologic data were analyzed.

Results

Ultrasonographic results showed that the thickness of femorotibial synovium was increased with modeling time

with significant differences among groups (

0.01). There was a significant difference in PDI score between the M4 and M8 groups (

0.05). A positive correlation was found between synovial thickness and pathologic score (

=0.887

0.01). Positive rate of CEUS was higher than that of PDI in both the M4 and M8 groups (8/12

3/12

12/12

10/12). Among the TIC data

the values of PI and AUC in the synovial tissues were lower than those of surrounding fat tissues (

0.01). There were positive correlations of the PI value

AUC value and PDI score of the synovial tissue with synovial vascular bed area index (

=0.664

0.01;

=0.711

0.01;

=0.483

0.05).

Conclusion

Grey scale ultrasonography can reflect the pathological changes of synovitis. CEUS is more sensitive than PDI in reflecting the formation of early angiogenesis in the synovium

and is valuable in the early dynamic detection of inflammation activity in RA.

关键词

Keywords

references

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