Effect of miR-221 on growth of transplantation tumor of neuroblastoma cell line SH-SY5Y in nude mice

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Effect of miR-221 on growth of transplantation tumor of neuroblastoma cell line SH-SY5Y in nude mice

Basic Medicine | 更新时间：2026-01-09

- Effect of miR-221 on growth of transplantation tumor of neuroblastoma cell line SH-SY5Y in nude mice
- Journal of Third Military Medical University Vol. 40, Issue 15, Pages: 1370-1374(2018)
- 作者机构：
  
  400014 重庆, 重庆医科大学附属儿童医院临床分子医学中心, 儿童发育疾病研究教育部重点实验室, 儿科学重庆市重点实验室, 重庆市(儿童发育重大疾病诊治与预防)国际科技合作基地
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Young Scholars of China(81201543)
- DOI：10.16016/j.1000-5404.201801190
  CLC： R394.3;R730.23;R730.264
- Received：23 January 2018，
  
  Revised：2018-02-08，
  
  Published：15 August 2018
- 稿件说明：
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Fangjie LIU, Xiaoyan HE, Zhenglan TAN, et al. Effect of miR-221 on growth of transplantation tumor of neuroblastoma cell line SH-SY5Y in nude mice[J]. Journal of Third Military Medical University, 2018, 40(15): 1370-1374.
DOI：

Fangjie LIU, Xiaoyan HE, Zhenglan TAN, et al. Effect of miR-221 on growth of transplantation tumor of neuroblastoma cell line SH-SY5Y in nude mice[J]. Journal of Third Military Medical University, 2018, 40(15): 1370-1374. DOI： 10.16016/j.1000-5404.201801190.

摘要

目的

2

研究高表达miR-221对神经母细胞瘤细胞SH-SY5Y裸鼠移植瘤生长的影响及其可能的分子机制。

方法

2

将18只裸鼠按照随机区组法分成3组：SH-SY5Y组（空白对照组）、miR-221-up组（实验组）和miRNA-NC组（阴性对照组），分别接种SH-SY5Y细胞、稳定转染miR-221的SH-SY5Y-miR-221细胞及稳定转染对照质粒的SH-SY5Y-miRNA-NC细胞，观察裸鼠体内成瘤及生长情况，30 d后处死取瘤体测量肿瘤体积、质量；qRT-PCR检测各组肿瘤组织中miR-221和MYCN mRNA水平的表达；Western blot和免疫组织化学法检测肿瘤组织中MYCN蛋白的表达水平。

结果

2

与SH-SY5Y组相比，miR-221-up组裸鼠的瘤体体积和质量明显增加（

P

<

0.01），miRNA-NC组无统计学差异（

P

>

0.05）；与SH-SY5Y组相比，miR-221-up组中miR-221、MYCN mRNA以及MYCN蛋白的表达明显升高（

P

<

0.01），而miRNA-NC组与SH-SY5Y组相比无统计学差异（

P

>

0.05）。

结论

2

miR-221可能通过上调MYCN的表达，促进SH-SY5Y细胞在裸鼠体内的生长。

Abstract

Objective

2

To investigate the effect and potential underlying mechanisms of miR-221 on the growth of transplantation tumor of neuroblastoma cell line SH-SY5Y in nude mice.

Methods

2

Eighteen nude mice were randomly divided into SH-SY5Y group (blank control group)

miR-221-up group (SH-SY5Y cells with miR-221 overexpression

experimental group) and miRNA-NC group (SH-SY5Y cells with miR-221 scramble siRNA

negative control group). Then SH-SY5Y cells

and stable transfected cells over-expressing miR-221 and miRNA-NC miRNA were respectively injected subcutaneously in the back of nude mice. The growth of tumor masses was observed. In 30 d after cell transplantation

the mice were sacrificed

and the tumor masses were taken for the weight and volume. Then the mRNA expression levels of miR-221 and MYCN in the tumor masses were analyzed by qRT-PCR. The protein expression of MYCN was measured by Western blotting and immunohistochemical assay.

Results

2

Significant increases in tumor size and weight were observed in the tumor masses of the miR-221-up group than those of the SH-SY5Y group (

P

<

0.05)

but no such differences were seen when compared with those of the miRNA-NC group (

P

>

0.05). The expression levels of miR-221 and MYCN mRNAs and MYCN protein were markedly increased in miR-221-up group than the SH-SY5Y group (

P

<

0.01)

but there were no such differences in the above expression levels between the SH-SY5Y group and miRNA-NC group (

P

>

0.05).

Conclusion

2

MiR-221 could promote the growth of SH-SY5Y neuroblastoma cells xenografts through enhancing MYCN expression.

关键词

Keywords

references

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[HE X Y, CHEN H, et al . MiR-221 promotes N-MYC expression and cell Proliferation in neuroblas-toma SH-SY5Y cells[J ] . Basic Clinical Med, 2016, 36(10): 1329-1334. DOI: 10.16352/j.issn.1001-6325.2016.10.002 http://dx.doi.org/10.16352/j.issn.1001-6325.2016.10.002 ..

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SCHULTE J H, HORN S, OTTO T, et al . MYCN regulates oncogenic MicroRNAs in neuroblastoma[J ] .Int J Cancer, 2008, 122(3):699-704. DOI:10.1002/ijc.23153.

KNEITZ B, KREBS M, KALOGIROU C, et al . Survival in patients with high-risk prostate cancer is predicted by miR-221, which regulates proliferation, apoptosis, and invasion of prostate cancer cells by inhibiting IRF2 and SOCS3[J ] .Cancer Res, 2014, 74(9):2591-2603. DOI:10.1158/0008-5472.CAN-13-1606.

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