Expression of miR-140 in mouse inner ear hair cells and its regulation on synaptic protein Dynamin-1

Xiaolin TANG; Yang LI; Fei YU; Lifeng JIA; Huajun LI; Wei YUAN

doi:10.16016/j.1000-5404.201801142

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Expression of miR-140 in mouse inner ear hair cells and its regulation on synaptic protein Dynamin-1

更新时间：2026-01-09

- Expression of miR-140 in mouse inner ear hair cells and its regulation on synaptic protein Dynamin-1
- Journal of Third Military Medical University Vol. 40, Issue 11, Pages: 966-972(2018)
- 作者机构：
- 作者简介：
- 基金信息：
  
  Supported by the General Program of National Natural Science Foundation of China(81470694);Supported by the General Program of National Natural Science Foundation of China(81271080);the Project of Southwest Hospital(JCZD-39)
- DOI：10.16016/j.1000-5404.201801142
  CLC：
- Received：17 January 2018，
  
  Published：2018-06
- 稿件说明：
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Xiaolin TANG, Yang LI, Fei YU, et al. Expression of miR-140 in mouse inner ear hair cells and its regulation on synaptic protein Dynamin-1[J]. Journal of Third Military Medical University, 2018, 40(11): 966-972.
DOI：

Xiaolin TANG, Yang LI, Fei YU, et al. Expression of miR-140 in mouse inner ear hair cells and its regulation on synaptic protein Dynamin-1[J]. Journal of Third Military Medical University, 2018, 40(11): 966-972. DOI： 10.16016/j.1000-5404.201801142.

摘要

Abstract

Objective

To investigate the expression and distribution of miR-140 in mouse inner ear hair cells and its targeting regulation on Dnm1

the gene encoding synaptophysin Dynamin-1.

Methods

After the cochlear basement membrane was harvested from healthy adult C57BL/6J mice (8 weeks old)

immunofluorescence staining and

in situ

hybridization were used to observe the expression and location of Dynamin-1 and miR-140. The luciferase reporter gene vector was constructed and then transfected into 293T cells to determine whether Dnm1 is the target gene of miR-140. Adeno-associated viral vectors carrying miR-140 precursor and blank gene were injected into the cochlea of the mice

via

circular window membrane. After 14 d

the mRNA expression of miR-140 and Dnm1 was detected by RT-PCR

and Dynamin

-1 protein was detected by Western blotting.

Results

miR-140 was expressed in the cytoplasm of inner and outer hair cells

and Dynamin-1 was mainly expressed in the cytoplasm of inner hair cells. The luciferase activity assay showed that miR-140 could inhibit the enzyme reporter activity of 3'UTR of Dnm1 (

0.05). The mRNA expression of miR-140 was significantly increased after transfection of AAV-miR-140

while the mRNA level of Dnm1 and its protein level were decreased significantly (

0.05).

Conclusion

Dnm1 is the target gene of miR-140

and the latter can inhibit the expression of Dynamin-1 in inner ear hair cells

suggesting that it may negatively regulate the process of synaptic vesicle endocytosis of inner hair cells by inhibiting Dynamin

and then affect the transmission of auditory signals.

关键词

Keywords

references

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