Fibrin glue and triamcinolone acetonide prevent postoperative esophageal stenosis after endoscopic submucosal dissection in a canine model

Yijun JIA; Hongbo WU; Ling WANG; Yusi QIN; Guiyong PENG

doi:10.16016/j.1000-5404.201801131

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Fibrin glue and triamcinolone acetonide prevent postoperative esophageal stenosis after endoscopic submucosal dissection in a canine model

更新时间：2026-01-09

- Fibrin glue and triamcinolone acetonide prevent postoperative esophageal stenosis after endoscopic submucosal dissection in a canine model
- Journal of Third Military Medical University Vol. 40, Issue 11, Pages: 979-983(2018)
- 作者机构：
- 作者简介：
- 基金信息：
  
  Supported by the General Program of National Natural Science Foundation of China(81070384);the General Program of National Natural Science Foundation of China(81470907);the Major Technical Innovation Program of First Affiliated Hospital of Third Military Medical University(SWH2016ZDCX2011)
- DOI：10.16016/j.1000-5404.201801131
  CLC：
- Received：02 January 2018，
  
  Published：2018-06
- 稿件说明：
移动端阅览
Yijun JIA, Hongbo WU, Ling WANG, et al. Fibrin glue and triamcinolone acetonide prevent postoperative esophageal stenosis after endoscopic submucosal dissection in a canine model[J]. Journal of Third Military Medical University, 2018, 40(11): 979-983.
DOI：

Yijun JIA, Hongbo WU, Ling WANG, et al. Fibrin glue and triamcinolone acetonide prevent postoperative esophageal stenosis after endoscopic submucosal dissection in a canine model[J]. Journal of Third Military Medical University, 2018, 40(11): 979-983. DOI： 10.16016/j.1000-5404.201801131.

摘要

Abstract

Objective

To investigate the preventive efficacy of fibrin glue supplementation with triamcinolone acetonide in a dog model of post-endoscopic esophageal dissection (ESD).

Methods

Twenty 12-month-old domestic dogs in good condition (both sexes

12 to 15 kg)

were randomly divided into 4 groups (

=5):fibrin glue-triamcinolone acetonide treatment group (FT)

fibrin glue treatment group (F)

triamcinolone acetonide treatment group (T) and control group. ESD model was inflicted by exfoliating three-quarters circumference of esophageal mucosa in 30~34 cm away from the incisor. Then fibrin glue suspensions with triamcinolone acetonide

fibrin glue solution or triamcinolone acetonide solution were correspondingly sprayed to cover the ulcer wound after ESD

respectively

and no drug was applied for the control group. Gross observation

stenosis rate

and HE staining and immunohistochemical examination of α-SMA for the stenosis tissues were performed in 6 weeks after ESD.

Results

Mucosal folds were sighted under endoscopy but in which a thinner esophageal wall was noted in macropathology. The dogs in the FT group gained a lower mucosal stenosis rate as to the dogs in the T group (

0.05) and control group (

0.01)

and a similar lower rate happened in the comparison with the dogs from the F group to the control group (

0.05). HE staining displayed atrophy in the muscularis propria

extensive myofibroblasts and collagen fibers in submucosa layer in the control group. Compared with the FT group

the expression level of α-SMA was augmented in the T group and control group (

0.01)

as well as in the T group (

0.01) and control group (

0.05) when compared with the F group.

Conclusion

Spraying fibrin glue-triamcinolone acetonide suspensions or fibrin glue alone can relieve esophageal post-ESD stenosis

probably

via

alleviating submucosa fibrosis and inflammation

but a single dose of triamcinolone acetonide does not work.

关键词

Keywords

references

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