<italic style="font-style: italic">Lrg1</italic> deficiency causes abnormal spermatogenesis and steroidogenesis in mice

Shiwei XIAO; Jie HAO; Chong LI; Xiaogang LIAO

doi:10.16016/j.1000-5404.201801114

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Lrg1 deficiency causes abnormal spermatogenesis and steroidogenesis in mice

更新时间：2026-01-09

- Lrg1 deficiency causes abnormal spermatogenesis and steroidogenesis in mice
- Journal of Third Military Medical University Vol. 40, Issue 16, Pages: 1483-1491(2018)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(30901062)
- DOI：10.16016/j.1000-5404.201801114
  CLC：
- Received：25 January 2018，
  
  Published：30 August 2018
- 稿件说明：
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Shiwei XIAO, Jie HAO, Chong LI, et al. Lrg1 deficiency causes abnormal spermatogenesis and steroidogenesis in mice[J]. Journal of Third Military Medical University, 2018, 40(16): 1483-1491.
DOI：

Shiwei XIAO, Jie HAO, Chong LI, et al. Lrg1 deficiency causes abnormal spermatogenesis and steroidogenesis in mice[J]. Journal of Third Military Medical University, 2018, 40(16): 1483-1491. DOI： 10.16016/j.1000-5404.201801114.

摘要

Abstract

Objective

To investigate the potential role of leucine-rich-alpha-2-glycoprotein 1 (

Lrg1

) in the biological functions of the testis by

Lrg1

deficiency mice.

Methods

Lrg1

knock-out mice were generated using Crispr/cas9 technology.Six 4-month-old

Lrg1

knock-out mice and 6 wild type mice served as experimental group and control group

respectively.Then

testicular damage was evaluated by HE staining.Serum levels of luteinizing hormone (LH) and testosterone

and intratesticular testosterone level from testis homogenates were measured with ELISA.High throughput Global gene expression analysis was performed for transcriptional levels of the genes in the testis.

Results

LRG1 was expressed in the spermatogonial cells and spermatocytes.Western blotting and immunofluorescence assay showed that the expr

ession of

Lrg1

was absent in the testis of

Lrg1

knock-out mice.The knock-out of

Lrg1

resulted in the development of testis

including decreased diameter of seminiferous tubules (196.22±27.88

183.67±26.32 μm

0.05)

increased degenerated germ cells (26.17±5.03

196.00±40.34

0.01)

reduced average number of round spermatids per seminiferous tubule (76.00±12.45

60.00±11.40 cells/tubule

0.01)

lowered serum testosterone level (2.90±0.92

1.90±0.29 ng/mL

0.05)

and decreased amount and vitality of spermatozoa in the epididymis.Bioinformatics analysis revealed that the altered gene expression induced by

Lrg1

deficiency mainly involved in meiosis

mitosis

chromosome segregation

spermatogenesis

and metabolism processes.In addition

48 members of the C2H2-type zinc finger protein family and 17 miRNAs were identified as deregulated in

Lrg1

-deficient testis

indicating that the testicular transcriptional regulatory network was altered.

Conclusion

Lrg1

takes part in spermatogenesis and steroidogenesis in the testis.

关键词

Keywords

references

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⁰

Lrg1 deficiency causes abnormal spermatogenesis and steroidogenesis in mice

DOI：10.16016/j.1000-5404.201801114

摘要

Abstract

关键词

Keywords

references