Long noncoding RNA TUG1 promotes γ radiation-induced senescence of human bone marrow mesenchymal stem cells <italic style="font-style: italic">in vitro</italic>

Jiang WU; Weiwei ZHANG; Zhongjun LI

doi:10.16016/j.1000-5404.201801017

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Long noncoding RNA TUG1 promotes γ radiation-induced senescence of human bone marrow mesenchymal stem cells in vitro

Basic Medicine | 更新时间：2026-01-09

- Long noncoding RNA TUG1 promotes γ radiation-induced senescence of human bone marrow mesenchymal stem cells in vitro
- Journal of Third Military Medical University Vol. 40, Issue 10, Pages: 904-908(2018)
- 作者机构：
  
  400037 重庆, 陆军军医大学(第三军医大学)第二附属医院输血科
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201801017
  CLC： R329.2; R818.023; R818.74
- Received：03 January 2018，
  
  Revised：2018-03-13，
  
  Published：30 May 2018
- 稿件说明：
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Jiang WU, Weiwei ZHANG, Zhongjun LI. Long noncoding RNA TUG1 promotes γ radiation-induced senescence of human bone marrow mesenchymal stem cells in vitro[J]. Journal of Third Military Medical University, 2018, 40(10): 904-908.
DOI：

Jiang WU, Weiwei ZHANG, Zhongjun LI. Long noncoding RNA TUG1 promotes γ radiation-induced senescence of human bone marrow mesenchymal stem cells in vitro[J]. Journal of Third Military Medical University, 2018, 40(10): 904-908. DOI： 10.16016/j.1000-5404.201801017.

摘要

目的

探讨lncRNA-TUG1在放射导致的骨髓间充质干细胞(bone marrow mesenchymal stem cells

BM-MSCs)衰老中所起的作用。

方法

实验分为lncRNA-TUG1干扰组、lncRNA-TUG1对照组，用γ射线造成放射损伤后，采用实时荧光定量PCR(RT-PCR)检测lncRNA-TUG1在2组的表达变化，采用Western blot检测2组的γ-H2AX蛋白反映DNA损伤，检测衰老相关蛋白p53表达以及β-半乳糖苷酶染色反映细胞衰老。

结果

γ射线放射显著增加了BM-MSCs中lncRNA-TUG1表达(

0.01)，BM-MSCs中迅速出现γ-H2AX磷酸化，p53蛋白水平升高，BM-MSCs衰老明显增加；而干扰lncRNA-TUG1后，放射后BM-MSCs的DNA损伤修复加快，p53蛋白水平下降，细胞衰老明显减少(

0.01)。

结论

放射可能通过lncRNA-TUG1促进BM-MSCs的衰老。

Abstract

Objective

To study the role of long noncoding RNA TUG1 (LncRNA-TUG1) in γ radiation-induced senescence of human bone marrow mesenchymal stem cells (BM-MSCs)

in vitro

Methods

Human BM-MSCs were transfected with a lentiviral vector carrying short-hairpin RNA (shRNA) targeting LncRNA-TUG1 (LncRNA-TUG1-SI) or with an empty lentiviral vector prior to γ-ray radiation. Cell senescence following the exposure was analyzed using SA-β-Gal staining

and Western blotting was used to detect the changes in the expressions of γ-H2AX (an indicator of DNA double-strand breaks) and p53 in the cells.

Results

γ-ray radiation at 9 Gy resulted in rapid γ-H2AX phosphorylation

significantly increased cell senescence

and increased protein levels enhanced expression of p53 and the expression of LncRNA-TUG1 in human BM-MSCs. In contrast

BM-MSCs transfected with LncRNA-TUG1 shRNA showed a fast recovery from γ radiation-induced DNA damage with significantly reduced cell senescence and decreased protein levels of p53.

Conclusions

LncRNA-TUG1 may promote senescence of BM-MSCs following γ radiation and can serve as a potential target for intervention of radiation-induced senescence of BM-MSCs.

关键词

Keywords

references

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Long noncoding RNA TUG1 promotes γ radiation-induced senescence of human bone marrow mesenchymal stem cells in vitro

DOI：10.16016/j.1000-5404.201801017

摘要

Abstract

关键词

Keywords

references