Establishment and evaluation of mouse model of induced membrane by femur bone defect

Wenkai WANG; Xin ZENG; Rui ZUO; Chao SUN; Yanqiu WANG; Song KE; Jie LI; Yue ZHOU

doi:10.16016/j.1000-5404.201712163

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Establishment and evaluation of mouse model of induced membrane by femur bone defect

Basic Medicine | 更新时间：2026-01-09

- Establishment and evaluation of mouse model of induced membrane by femur bone defect
- Journal of Third Military Medical University Vol. 40, Issue 9, Pages: 805-809(2018)
- 作者机构：
  
  1.400037 重庆, 陆军军医大学(第三军医大学)第二附属医院骨科
  2.400014 重庆, 重庆市药品技术审评认证中心
- 作者简介：
- 基金信息：
  
  the Clinical Research Project of Xinqiao Hospital of Third Military Medical University(2015YLC22)
- DOI：10.16016/j.1000-5404.201712163
  CLC： R-332;R322.71;R681.05
- Received：18 December 2017，
  
  Revised：2018-01-15，
  
  Published：15 May 2018
- 稿件说明：
移动端阅览
Wenkai WANG, Xin ZENG, Rui ZUO, et al. Establishment and evaluation of mouse model of induced membrane by femur bone defect[J]. Journal of Third Military Medical University, 2018, 40(9): 805-809.
DOI：

Wenkai WANG, Xin ZENG, Rui ZUO, et al. Establishment and evaluation of mouse model of induced membrane by femur bone defect[J]. Journal of Third Military Medical University, 2018, 40(9): 805-809. DOI： 10.16016/j.1000-5404.201712163.

摘要

目的

建立标准化成年小鼠股骨骨缺损诱导膜模型。

方法

取18只C57BL/6品系的小鼠

6只测量小鼠股骨解剖参数

12只小鼠制备长度为3 mm的股骨干中段骨缺损。应用骨水泥髓内钉固定股骨

术后1周X线片验证内固定效果; 术后4周获取骨水泥表面诱导膜组织

运用组织学染色观察诱导膜组织和正常骨膜组织的差异。

结果

术后1周X线片证实1只小鼠出现内固定失效; 术后4周

HE染色证实11只小鼠骨水泥周围均有典型诱导膜形成

建模成功率91.7%。

结论

通过PMMA骨水泥髓内钉系统成功制备了成年小鼠股骨骨缺损诱导膜模型

4周后诱导膜形成良好

组织学证实其为小鼠诱导膜组织。

Abstract

Objective

To establish a simple

reproductive and standardized induced membrane model by femur bone defect in mice.

Methods

Eighteen C57BL/6 mice were used in this study.Six mice were employed for anatomic parameters of the femur.Segmental defect of 3 mm in size were inflicted in the right femur of the rest 12 mice.An intramedullary nail with cement spacer was placed in the defect to offer the stability of femur.The fixation effect was evaluated at 1 week postoperatively by X-ray filming.In 4 weeks after operation

the induced membrane surrounding cement spacer was collected

and HE staining was used to observe the pathological differences between the induced membrane and normal periosteum.

Results

X-ray film demonstrated internal fixation fail was observed in 1 mouse.Induced membrane was formed around the cement spacer in the other 11 mice.HE staining confirmed the characteristics of induced membrane.Satisfactory models were created in 91.7% experimental mice.

Conclusion

An adult mouse model of induced membrane is successfully established by femur defect followed by intramedullary nail fixation with cement spacer system.Mature induced membrane could be obtained at 4 week after surgery in mice.This model of induced membrane is of high repeatability and confirmed by histology.

关键词

Keywords

references

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