Function of circular RNA hsa_circ_0000711 in hepatocellular carcinoma cell lines

Xia LIN; Yuanxin ZHU; Hangwei FU; Guangyao LI; Ping CHEN

doi:10.16016/j.1000-5404.201712097

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Function of circular RNA hsa_circ_0000711 in hepatocellular carcinoma cell lines

Basic Medicine | 更新时间：2026-01-09

- Function of circular RNA hsa_circ_0000711 in hepatocellular carcinoma cell lines
- Journal of Third Military Medical University Vol. 40, Issue 9, Pages: 788-794(2018)
- 作者机构：
  
  400042 重庆, 陆军军医大学(第三军医大学)第三附属医院(野战外科研究所)肝胆外科
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201712097
  CLC： R394.3;R730.23;R735.7
- Received：11 December 2017，
  
  Revised：2018-01-04，
  
  Published：15 May 2018
- 稿件说明：
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Xia LIN, Yuanxin ZHU, Hangwei FU, et al. Function of circular RNA hsa_circ_0000711 in hepatocellular carcinoma cell lines[J]. Journal of Third Military Medical University, 2018, 40(9): 788-794.
DOI：

Xia LIN, Yuanxin ZHU, Hangwei FU, et al. Function of circular RNA hsa_circ_0000711 in hepatocellular carcinoma cell lines[J]. Journal of Third Military Medical University, 2018, 40(9): 788-794. DOI： 10.16016/j.1000-5404.201712097.

摘要

目的

研究环状RNA hsa_circ_0000711在人肝癌细胞系中的表达以及对肝癌细胞生物学功能的影响。

方法

利用RT-qPCR检测环状RNA hsa_circ_0000711在肝癌细胞系的表达; 通过小干扰RNA特异性敲低hsa_circ_0000711的表达; 通过CCK-8实验检测肝癌细胞系的增殖情况; 利用流式细胞技术检测细胞周期及细胞凋亡。Western blot检测下调hsa_circ_0000711后Cyclin D1、CDK4、cleavaed Caspase 3及Bcl-2蛋白的表达水平。

结果

Hsa_circ_0000711在肝癌细胞HepG2和SMMC-7721中明显高表达(

0.05)。与对照组相比

hsa_circ_0000711干扰组的肝癌细胞增殖能力显著降低(

0.05)

处于G

期细胞增多(

0.05)

且凋亡细胞显著增加(

0.05)。干扰hsa_circ_0000711的表达后

肝癌细胞中Cyclin D1、CDK4和Bcl-2的蛋白表达量显著降低(

0.05)

cleavaed Caspase 3蛋白表达量显著升高(

0.05)。

结论

Hsa_circ_0000711可能通过调控细胞周期及凋亡影响肝癌细胞的增殖。

Abstract

Objective

To explore the expression profile of circular RNA (circRNA)

hsa_circ_0000711

in hepatocellular carcinoma (HCC) cell lines and determine the effect of hsa_circ_0000711 on the biological behaviors of HCC cells.

Methods

The expression level of Hsa_circ_0000711 were detected by real-time polymerase chain reaction assay (RT-qPCR) in HCC cell lines HepG2 and SMMC-7721 and human live cell line L02.Small RNA interference was used to knock down the expression of hsa_circ_0000711.Then CCK-8 assay was employed to detect the proliferation

and flow cytometry for cell cycle and apoptosis.The protein levels of Cyclin D1

CDK4

cleaved Caspase 3 and Bcl-2 were measured by Western blotting.

Results

Hsa_circ_0000711 was more strongly expressed in the HepG2 and SMMC-7721 cells than the L02 cells (

0.05).Down-regulation of hsa_circ_0000711 significantly suppressed the growth rate of HepG2 and SMMC-7721 cells (

0.05)

arrested the cells at cycle G

(

0.05)

and promoted cell apoptosis (

0.05).After hsa_circ_0000711 interference

the protein expression levels of Cyclin D1

CDK4 and Bcl-2 were decreased notably (

0.05)

but that of cleaved Caspase 3 was increased (

0.05).

Conclusion

Hsa_circ_0000711 may affect HCC cell proliferation by regulating cell cycle and cell apoptosis.

关键词

Keywords

references

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