Determination of ginsenoside Rg3 in rat plasma using high-performance liquid chromatography-tandem mass spectrometry

Rongfeng XIANG; Qing DAI; Yan ZHOU; Mingjie YU; Xiaodan LAI

doi:10.16016/j.1000-5404.201712007

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Determination of ginsenoside Rg3 in rat plasma using high-performance liquid chromatography-tandem mass spectrometry

Basic Medicine | 更新时间：2026-01-09

- Determination of ginsenoside Rg3 in rat plasma using high-performance liquid chromatography-tandem mass spectrometry
- Journal of Third Military Medical University Vol. 40, Issue 12, Pages: 1073-1078(2018)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院药剂科
- 作者简介：
- 基金信息：
  
  the Basic and Frontier Sci-Tech Research Program of Chongqing(CSTC 2015jcyjBX0058)
- DOI：10.16016/j.1000-5404.201712007
  CLC： R285.5;R965.2;R969.1
- Received：01 December 2017，
  
  Revised：2018-02-11，
  
  Published：2018-01
- 稿件说明：
移动端阅览
Rongfeng XIANG, Qing DAI, Yan ZHOU, et al. Determination of ginsenoside Rg3 in rat plasma using high-performance liquid chromatography-tandem mass spectrometry[J]. Journal of Third Military Medical University, 2018, 40(12): 1073-1078.
DOI：

Rongfeng XIANG, Qing DAI, Yan ZHOU, et al. Determination of ginsenoside Rg3 in rat plasma using high-performance liquid chromatography-tandem mass spectrometry[J]. Journal of Third Military Medical University, 2018, 40(12): 1073-1078. DOI： 10.16016/j.1000-5404.201712007.

摘要

目的

建立高效液相色谱-串联质谱(HPLC-MS/MS)法测定大鼠血浆中人参皂苷Rg3的浓度，用于大鼠体内人参皂苷Rg3的药代动力学研究。

方法

血浆样品经乙酸乙酯液-液提取。色谱柱为Inertsil ODS-3(2.1 mm×50 mm，5 μm)，流动相：乙腈：10 mmol/L乙酸铵水溶液(含0.1%甲酸

90 :10)；流速：0.4 mL/min；ESI离子源，负离子模式监测。6只大鼠灌胃给予人参皂苷Rg3 20 mg/kg后按预定时间点眼眶采血。采用DAS 3.3.1软件统计其药代动力学参数。

结果

血浆中人参皂苷Rg3的线性范围为2~400 ng/mL，日内、日间精密度及基质效应RSD均小于15%，主要的药代动力学参数：AUC

0-t

为(821.659±170.125)ng·h/mL，AUC

0-∞

为(912.468±190.653)ng·h/mL，

max

为(138.803±28.997)ng/mL，

1/2

为(2.803±0.263)h。

结论

建立的方法快速、灵敏、准确。适用于大鼠血浆中人参皂苷Rg3浓度的测定。

Abstract

Objective

To establish a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) system for determination of ginsenoside Rg3 content in rat plasma.

Methods

Venous blood samples were collected from the orbit of 6 rats at different time points after intragastric administration of 20 mg/kg ginsenosides Rg3. Plasma was isolated and the samples were extracted with ethyl acetate

followed by chromatographic separation using a C18 column (2.1 mm×50 mm

5μm) with acetonitrile-ammonium acetate (10 mmol/L

containing 0.1% formic acid) (90 :10) as the mobile phase at a flow rate 0.4 mL/min. The detection was accomplished with the electrospray ionization source operated in the negative ionization mode. The pharmacokinetic parameters were computed using DAS 3.3.1 pharmacokinetic software.

Results

The linear calibration curves of ginsenoside Rg3 in rat plasma were obtained within the concentration range of 2~400 ng/mL. The inter- and intra-batch RSDs and the matrix effect of ginsenoside Rg3 detection were all below 15%. Analysis of the pharmacokinetic parameters showed that in rats

ginsenoside Rg3 had an AUC

0-t

of 821.659±170.125 ng·h/mL

an AUC

0-∞

of 912.468±190.653 ng·h/mL

max

of 138.803±28.997 ng/mL

and a

1/2

of 2.803±0.263 h.

Conclusion

This HPLC-MS/MS system allows rapid

sensitive and accurate determination of ginsenoside Rg3 in rat plasma.

关键词

Keywords

references

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