Expression of DNA methyltransferase is significantly increased in the colonic mucosa of patients with slow transit constipation

Lei XIAO; Yue TIAN; Zhigang KE; Yong ZHANG; Weidong TONG

doi:10.16016/j.1000-5404.201711221

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Expression of DNA methyltransferase is significantly increased in the colonic mucosa of patients with slow transit constipation

Basic Medicine | 更新时间：2026-01-09

- Expression of DNA methyltransferase is significantly increased in the colonic mucosa of patients with slow transit constipation
- Journal of Third Military Medical University Vol. 40, Issue 8, Pages: 705-710(2018)
- 作者机构：
  
  400042 重庆，陆军军医大学(第三军医大学)第三附属医院野战外科研究所普通外科
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81270461);the National Natural Science Foundation of China(81570483);the National Natural Science Foundation of China(81770541)
- DOI：10.16016/j.1000-5404.201711221
  CLC： R322.45;R345.5;R574.62
- Received：27 November 2017，
  
  Revised：2017-12-19，
  
  Published：30 April 2018
- 稿件说明：
移动端阅览
Lei XIAO, Yue TIAN, Zhigang KE, et al. Expression of DNA methyltransferase is significantly increased in the colonic mucosa of patients with slow transit constipation[J]. Journal of Third Military Medical University, 2018, 40(8): 705-710.
DOI：

Lei XIAO, Yue TIAN, Zhigang KE, et al. Expression of DNA methyltransferase is significantly increased in the colonic mucosa of patients with slow transit constipation[J]. Journal of Third Military Medical University, 2018, 40(8): 705-710. DOI： 10.16016/j.1000-5404.201711221.

摘要

目的

研究DNA甲基转移酶(DNA methyltransferase

Dnmt)在慢传输性便秘(slow transit constipation

STC)结肠黏膜中的表达。

方法

收集2015年9月至2017年6月我科收治的8例STC患者乙状结肠标本，采用免疫组织化学染色、Western blot检测Dnmt1、Dnmt3a、Dnmt3b蛋白在结肠黏膜的表达情况，real-time qRT-PCR检测结肠黏膜Dnmt1、Dnmt3a、Dnmt3b mRNA相对表达量。对照组标本采用直肠癌患者术中切除的远离癌灶的近端乙状结肠。

结果

免疫组织化学染色结果显示：对照组Dnmt1、Dnmt3a、Dnmt3b IOD值分别为(705±362)、(538±206)、(359±238)，STC组Dnmt1、Dnmt3a、Dnmt3b IOD值分别为(4 077±1 219)、(4 214±1 858)、(3 026±1 619)，两组比较差异有统计学意义(

0.05)；Western blot检测结果显示：对照组Dnmt1、Dnmt3a、Dnmt3b蛋白相对表达量分别为(0.663± 0.349)、(0.250±0.159)、(0.330±0.195)，STC组Dnmt1、Dnmt3a、Dnmt3b蛋白相对表达量分别为(1.475±0.372)、(1.035±0.362)、(0.977±0.258)，两组比较差异有统计学意义(

0.05)；Real-time qRT-PCR结果显示：对照组Dnmt1、Dnmt3a、Dnmt3b mRNA相对表达量分别为(0.285±0.121)、(0.069± 0.021)、(0.512±0.112)，STC组Dnmt1、Dnmt3a、Dnmt3b mRNA相对表达量分别为(0.563±0.172)、(0.340±0.083)、(0.964±0.219)，两组比较差异有统计学意义(

0.05)。

结论

STC患者结肠黏膜Dnmt1、Dnmt3a、Dnmt3b表达显著增高，可能是STC发病的重要环节。

Abstract

Objective

To investigate the expression of DNA methyltransferase (Dnmt) in the colonic mucosa of patients with slow transit constipation (STC).

Methods

Surgical specimens of the sigmoid colon were collected from 8 patients with STC and 8 patients with rectal cancer admitted in our hospital between September

2015 and June

2017. Only the adjacent colon tissues (at least 10 cm from the tumor margin) from the rectal cancer patients were used to serve as the control. The expressions of Dnmt1

Dnmt3a and Dnmt3b proteins in the colonic mucosa were detected using immunochemistry and Western blotting

and their mRNA expressions were quantified with real-time qRT-PCR.

Results

The results of immunochemical staining showed that the integral optical densities (IOD) of Dnmt1

Dnmt3a and Dnmt3b expressions in the control group were 705±362

538±206

and 359±238

respectively

significantly lower than those in STC group (4 077±1 219

4 214±1 858

and 3 026±1 619

respectively;

0.05). Western blotting also showed significantly lower relative expressions of Dnmt1

Dnmt3a and Dnmt3b proteins in the control group than in STC group (0.663±0.349

0.250±0.159

and 0.330±0.195

1.475±0.372

1.035±0.362

and 0.977±0.258

respectively;

0.05). Real-time qRT-PCR yielded consistent results of the relative expressions of Dnmt1

Dnmt3a and Dnmt3b mRNAs in the control and STC groups (0.285±0.121

0.069±0.021

and 0.512±0.112

0.563±0.172

0.340±0.083

and 0.964±0.219

respectively;

0.05).

Conclusion

High expressions of Dnmt1

Dnmt3a and Dnmt3b in the colonic mucosa may be an important factor in the pathogenesis of STC.

关键词

Keywords

references

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