Effect of miR-455-3p overexpression on migration and invasion of colon cancer HCT116 cells and possible mechanism

Yijuan LIU; Lin ZHANG; Jiantao ZHENG; Liangqing LI

doi:10.16016/j.1000-5404.201711074

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Effect of miR-455-3p overexpression on migration and invasion of colon cancer HCT116 cells and possible mechanism

Basic Medicine | 更新时间：2026-01-09

- Effect of miR-455-3p overexpression on migration and invasion of colon cancer HCT116 cells and possible mechanism
- Journal of Third Military Medical University Vol. 40, Issue 14, Pages: 1286-1290(2018)
- 作者机构：
  
  1.350000 福州，福建医科大学附属第一医院：消化内科
  2.350000 福州，福建医科大学附属第一医院：胃肠外科
- 作者简介：
- 基金信息：
  
  the Medical Science Innovation Project of Fujian Province(2017-CX-29)
- DOI：10.16016/j.1000-5404.201711074
  CLC： R394.3; R730.23; R735.35
- Received：10 November 2017，
  
  Revised：2018-04-15，
  
  Published：30 July 2018
- 稿件说明：
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Yijuan LIU, Lin ZHANG, Jiantao ZHENG, et al. Effect of miR-455-3p overexpression on migration and invasion of colon cancer HCT116 cells and possible mechanism[J]. Journal of Third Military Medical University, 2018, 40(14): 1286-1290.
DOI：

Yijuan LIU, Lin ZHANG, Jiantao ZHENG, et al. Effect of miR-455-3p overexpression on migration and invasion of colon cancer HCT116 cells and possible mechanism[J]. Journal of Third Military Medical University, 2018, 40(14): 1286-1290. DOI： 10.16016/j.1000-5404.201711074.

摘要

目的

探讨miRNA-455-3p对HCT116结肠癌细胞迁移、侵袭的影响及机制研究。

方法

采用脂质体Lipofectamine

3000将miR-455-3p mimics和miR-455-3p NC转入HCT116结肠癌细胞中，Real-time PCR检测细胞中miR-455-3p表达，MTT法检测细胞活力，划痕实验检测细胞迁移能力，Transwell实验检测细胞侵袭能力，Western blot检测细胞中基质金属蛋白酶2(matrix metalloproteinases 2，MMP2)、MMP9、磷脂酰肌醇3激酶(phosphatidylinositol 3 kinase，PI3K)/蛋白激酶B(protein kinase B，AKT)信号通路相关蛋白的表达。

结果

与miR-455-3p NC组比较，miR-455-3p mimics组miR-455-3p表达量显著提高(

＜0.01)，细胞活力、迁移能力、侵袭能力均显著降低(

＜0.01)，MMP2、MMP9、PI3K及p-AKT表达量均显著下调(

＜0.01)。

结论

miR-455-3p mimics可能通过阻断PI3K/AKT信号通路抑制HCT116细胞的迁移及侵袭。

Abstract

Objective

To investigate the effect of miR-455-3p overexpression on the migration and invasion of colon cancer HCT116 cells and explore the underlying mechanism.

Methods

HCT116 cells were transfected with miR-455-3p mimics or miR-455-3p NC using Lipofectamine

3000

and the expression of miR-455-3p in t

he cells was detected with real-time PCR. The changes in the viability of the transfected cells was assessed using MTT assay

and the alterations in the cell migration and invasion abilities of the cells were investigated using scratch wound healing assay and Transwell assay

respectively. The expression levels of matrix metalloproteinases 2 (MMP2)

MMP9

and the proteins related with the phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) signaling pathway were detected using Western blotting.

Results

Compared with miR-455-3p NC

transfection with miR-455-3p mimics resulted in significantly up-regulated expression of miR-455-3p (

0.01)

obviously lowered cell viability and migration and invasion abilities (

0.01)

and significant down-regulation of the expression of MMP2

MMP9

PI3K and p-AKT (

0.01) in HCT116 cells.

Conclusion

Overexpression of miR-455-3p can inhibit the migration and invasion of HCT116 cells

in vitro

possibly by blocking the PI3K/AKT signaling pathway.

关键词

Keywords

references

刘丽丽, 李咏梅.多层螺旋CT和MRI对结肠癌术前评估的研究进展[J].中国医学影像学杂志, 2013, 21 (6): 475-477.DOI:10.3969/j.issn.1005-5185.2013.06.021.

LIU L L, LI Y M. Advance in preoperative evaluation of colon cancer by multi-slice spiral CT and MRI [J]. Chin J Med Imag, 2013, 21(6): 475-477. DOI: 10.3969/j.issn.1005-5185.2013.06.021

康清杰, 向征.结肠癌筛查和诊疗的研究进展[J].重庆医学, 2015, 44 (28): 4001-4003.DOI:10.3969/j.issn.1671-8348.2015.28.037.

KANG Q J, XIANG Z. Progress in screening and diagnosis of colon cancer [J]. Chongqing Med, 2015, 44 (28): 4001-4003. DOI: 10.3969/j.issn.1671-8348.2015.28.037

刘静, 黄红.结肠癌发病机制中原癌基因与抑癌基因研究及相关药物治疗进展[J].中国药房, 2014, 25(45): 4302-4304.DOI:10.6039/j.issn.1001-0408.2014.45.25.

LIU J, HUANG H. Advances in the study of proto-oncogene, antioncogene and related drugs in the pathogenesis of colon cancer[J]. China Pharm, 2014, 25(45): 4302-4304. DOI: 10.6039/j.issn.1001- 0408.2014.45.25

CHAKRABORTY C, CHIN K Y, DAS S. miRNA-regulated cancer stem cells: understanding the property and the role of miRNA in carcinogenesis [J]. Tumour Biol, 2016, 37 (10): 13039-13048. DOI: 10.1007/s13277-016-5156-1.

RAMALINGAM S, SUBRAMANIAM D, ANANT S. Manipulating miRNA expression: a novel approach for colon cancer prevention and chemotherapy [J]. Curr Pharmacol Rep, 2015, 1 (3): 141-153. DOI: 10.1007/s40495-015-0020-3.

AHMED F E. miRNA as markers for the diagnostic screening of colon cancer [J]. Expert Rev Anticancer Ther, 2014, 14 (4): 463-485. DOI: 10.1586/14737140.2014.869479.

KANEMATSU S, TANIMOTO K, SUZUKI Y, et al. Screening for possible miRNA-mRNA associations in a colon cancer cell line [J]. Gene, 2014, 533 (2): 520-531. DOI: 10.1016/j.gene.2013.08.005.

ZHAO Y, YAN M, YUN Y, et al. MicroRNA-455-3p functions as a tumor suppressor by targeting eIF4E in prostate cancer [J]. Oncol Rep, 2017, 37 (4): 2449-2458. DOI: 10.3892/or.2017.5502.

LI Z, MENG Q, PAN A, et al. MicroRNA-455-3p promotes invasion and migration in triple negative breast cancer by targeting tumor suppressor EI24 [J]. Oncotarget, 2017, 8 (12): 19455-19466. DOI: 10.18632/oncotarget.14307.

YANG H, WEI Y N, ZHOU J, et al. MiR-455-3p acts as a prognostic marker and inhibits the proliferation and invasion of esophageal squamous cell carcinoma by targeting FAM83F[J]. Eur Rev Med Pharmacol Sci, 2017, 21 (14): 3200-3206.

张康, 梅倩, 李祥, 等.过表达miR-455抑制宫颈癌细胞SiHa的生长[J].中国生物化学与分子生物学报, 2012, 28 (10): 935-939.DOI:10.13865/j.cnki.cjbmb.2012.10.008.

ZHANG K, MEI Q, LI X, et al. Overexpression of miR-455 inhibits proliferation in SiHa cells [J]. China J Biochem Mol Bio, 2012, 28 (10): 935-939. DOI: 10.13865/j.cnki.cjbmb.2012.10.008

ZHENG J, LIN Z, ZHANG L, et al. MicroRNA-455-3p inhibits tumor cell proliferation and induces apoptosis in HCT116 human colon cancer cells [J]. Med Sci Monit, 2016, 22: 4431-4437

EKBOTE A V, DANDA S, ZANKL A, et al. Patient with mutation in the matrix metalloproteinase 2 (MMP2) gene a case report and review of the literature [J]. J Clin Res Pediatr Endocrinol, 2014, 6 (1): 40-46. DOI: 10.4274/Jcrpe.1166.

KIM E Y, SONG H Y, KIM J C, et al. MMP-9 expression after metallic stent placement in patients with colorectal cancer: association with in-stent restenosis [J]. Radiology, 2014, 271 (3): 901-908. DOI: 10.1148/radiol.13121794.

DAMODHARAN U, GANESAN R, RADHAKRISHNAN U C. Expression of MMP2 and MMP9 (gelatinases A and B) in human colon cancer cells [J]. Appl Biochem Biotechnol, 2011, 165 (5-6): 1245-1252. DOI: 10.1007/s12010-011-9342-8.

CHEN S, FISHER R C, SIGNS S, et al. Inhibition of PI3K/Akt/mTOR signaling in PI3KR2-overexpressing colon cancer stem cells reduces tumor growth due to apoptosis [J]. Oncotarget, 2017, 8 (31): 50476-50488. DOI: 10.18632/oncotarget.9919.

MARTINI M, DE SANTIS M C, BRACCINI L, et al. PI3K/AKT signaling pathway and cancer: an updated review [J]. Ann Med, 2014, 46 (6): 372-383. DOI: 10.3109/07853890.2014.912836.

MALINOWSKY K, NITSCHE U, JANSSEN K P, et al. Activation of the PI3K/AKT pathway correlates with prognosis in stage Ⅱ colon cancer [J]. Br J Cancer, 2014, 110 (8): 2081-2089. DOI: 10.1038/bjc.2014.100.

TOKUHIRA N, KITAGISHI Y, SUZUKI M, et al. PI3K/AKT/PTEN pathway as a target for Crohn's disease therapy (Review) [J]. Int J Mol Med, 2015, 35 (1): 10-16. DOI: 10.3892/ijmm.2014.1981.

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