Preparation and identification of <sup>131</sup>I-RP215 McAb molecular probe and its application for radioimmunoimaging in lung adenocarcinoma-bearing nude mice

Mengxue WU; Chengming LEI; Zuwen TAN; Hui ZHANG; Jiali TANG; Dong DUAN

doi:10.16016/j.1000-5404.201711070

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Preparation and identification of ¹³¹I-RP215 McAb molecular probe and its application for radioimmunoimaging in lung adenocarcinoma-bearing nude mice

Basic Medicine | 更新时间：2026-01-09

- Preparation and identification of ¹³¹I-RP215 McAb molecular probe and its application for radioimmunoimaging in lung adenocarcinoma-bearing nude mice
- Journal of Third Military Medical University Vol. 40, Issue 7, Pages: 584-589(2018)
- 作者机构：
  
  400016 重庆, 重庆医科大学附属第一医院核医学科
- 作者简介：
- 基金信息：
  
  the General Program of Chongqing Natural Science Foundation of China(CSTC2014jcyjA10012);the Project of Science and Technology Plan of Chongqing(渝科发计〔2014〕21号);the National Clinical Key-discipline Construction Program of China(国卫办医函〔2013〕544号)
- DOI：10.16016/j.1000-5404.201711070
  CLC： R392-33;R734.2;R817.4
- Received：10 November 2017，
  
  Revised：2017-12-06，
  
  Published：15 April 2018
- 稿件说明：
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Mengxue WU, Chengming LEI, Zuwen TAN, et al. Preparation and identification of ¹³¹I-RP215 McAb molecular probe and its application for radioimmunoimaging in lung adenocarcinoma-bearing nude mice[J]. Journal of Third Military Medical University, 2018, 40(7): 584-589.
DOI：

Mengxue WU, Chengming LEI, Zuwen TAN, et al. Preparation and identification of ¹³¹I-RP215 McAb molecular probe and its application for radioimmunoimaging in lung adenocarcinoma-bearing nude mice[J]. Journal of Third Military Medical University, 2018, 40(7): 584-589. DOI： 10.16016/j.1000-5404.201711070.

摘要

目的

制备靶向肺腺癌细胞表面碳水化合物相关抗原(carbohydrate antigen 215，CA215)的核素分子探针

131

I-RP215单克隆抗体(monoclonal antibody

McAb)，鉴定其理化性质并探讨其在荷瘤裸鼠模型中的体内分布情况和放射免疫显像特点。

方法

采用氯胺T法对McAb RP215进行

131

I标记，Sephadex G25M柱纯化标记抗体，并鉴定其理化特性，观察该抗体标记物在荷A549裸鼠模型中的生物分布及放射免疫显像情况。

结果

131

I-RP215 McAb标记率为(91.03±2.36)%，纯化后的放化纯度为(93.15±1.40)%，比活度(3.37±0.42)MBq/μg；具有较好的稳定性及免疫活性。荷瘤裸鼠体内生物分布及SPECT显像结果显示，

131

I-RP215 McAb能够在肿瘤组织中选择性浓聚，

131

I-RP215 McAb作用24 h时，肿瘤/肌肉比达最高，且瘤体显影最清晰。

结论

成功制备了特性理想的

131

I-RP215 McAb分子探针，在荷人肺腺癌裸鼠模型中有较好的显像效果。

Abstract

Objective

To prepare a targeted probe for nuclide labeling of

131

I-RP215 monoclonal antibody (McAb) against carbohydrate-associated antigen

carbohydrate antigen 215 (CA215) which are expressed on the surface of lung adenocarcinoma cells

identify the physico-chemical properties of the probe

and to explore its distribution

in vivo

and radioimmunoimaging (RII) characteristics in tumor-bearing nude mice model.

Methods

McAb RP215 was labeled with

131

I by the chloramine T method

and then the products were purified with the Sephadex G25M column. The physico-chemical properties of the obtained McAbs were studied. Nude mice model bearing human lung adenocarcinoma was established by transplantation of A549 cells. Then the mice received the injection of

131

I-RP215 McAbs

via

tail vein

and the bio-distribution and RII characteristics were observed with single-photon emission computerized tomography (SPECT).

Results

The obtained

131

I-RP215 McAbs showed a labeling rate of (91.03±2.36)%

a radiochemical purity of (93.15±1.40)%

and a specific activity of 3.37±0.42 MBq/μg

and had good stabilities and immunological competence. Biodistribution and SPECT imaging showed

131

I-RP215 selectively accumulated in tumor tissue

and the target/non-target (T/NT) ratio reached the highest with the clearest tumor imaging at 24 h after injection of McAbs.

Conclusion

Molecular probes

131

I-RP215 McAbs

with ideal physicochemical properties are successfully prepared

and show satisfactory SPECT imaging in tumor-bearing nude mice.

关键词

Keywords

references

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⁰

Preparation and identification of 131I-RP215 McAb molecular probe and its application for radioimmunoimaging in lung adenocarcinoma-bearing nude mice

DOI：10.16016/j.1000-5404.201711070

摘要

Abstract

关键词

Keywords

references

Preparation and identification of ¹³¹I-RP215 McAb molecular probe and its application for radioimmunoimaging in lung adenocarcinoma-bearing nude mice