Expression of circRNA-IARS in pancreatic carcinoma and its clinical significance

Jie LI; Zhonghu LI; Peng JIANG; Xiaowu LI

doi:10.16016/j.1000-5404.201711069

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Expression of circRNA-IARS in pancreatic carcinoma and its clinical significance

Clinical Medicine | 更新时间：2026-01-09

- Expression of circRNA-IARS in pancreatic carcinoma and its clinical significance
- Journal of Third Military Medical University Vol. 40, Issue 13, Pages: 1229-1235(2018)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院肝胆外科研究所
- 作者简介：
- 基金信息：
  
  the Key Program of National Natural Science Foundation of China(81430063)
- DOI：10.16016/j.1000-5404.201711069
  CLC： R394.3;R730.23;R735.9
- Received：09 November 2017，
  
  Revised：2018-04-18，
  
  Published：15 July 2018
- 稿件说明：
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Jie LI, Zhonghu LI, Peng JIANG, et al. Expression of circRNA-IARS in pancreatic carcinoma and its clinical significance[J]. Journal of Third Military Medical University, 2018, 40(13): 1229-1235.
DOI：

Jie LI, Zhonghu LI, Peng JIANG, et al. Expression of circRNA-IARS in pancreatic carcinoma and its clinical significance[J]. Journal of Third Military Medical University, 2018, 40(13): 1229-1235. DOI： 10.16016/j.1000-5404.201711069.

摘要

目的

检测环状RNA-IARS(hsa_circ_0087493

circRNA-IARS)在胰腺癌组织中的表达水平并探讨其表达与临床病理特征及预后的关系。

方法

利用环状RNA(circular RNA

circRNA)芯片比较胰腺癌Hs 766T与Hs 766T-L2细胞circRNA表达差异，并用实时定量PCR(qRT-PCR)验证，结合生物信息学分析选择circRNA-IARS；用qRT-PCR检测25对胰腺癌组织及配对癌旁组织中circRNA-IARS和linar-IARS(线性IARS)的表达水平；同时检测115例胰腺癌中circRNA-IARS表达水平，并分析其与胰腺癌临床病例特征和预后的关系。

结果

circRNA芯片示Hs 66T-L2细胞中28条circRNA表达上调。circRNA-IARS在胰腺癌组织表达水平明显高于癌旁组织中(

＜0.05)，linar-IARS表达无差异(

0.05)；circRNA-IARS表达水平与淋巴结侵袭、血管侵袭、肝转移及肿瘤TNM分期有关(

＜0.05)，linar-IARS表达与临床病理特征无关(

0.05)；circRNA-IARS高表达组患者术后生存时间明显短于低表达组(

＜0.05)。

结论

circRNA-IARS在胰腺癌组织中高表达，与肿瘤淋巴结侵袭、血管侵犯、肝转移及TNM分期有关，并影响患者预后。

Abstract

Objective

To investigate the expression of circRNA-IARS in pancreatic carcinoma and its relation with the clinicopathological features and prognosis of the patients.

Methods

The differential expressions of circular RNA (circRNA) between pancreatic cancer cell lines Hs 766T and Hs 766T-L2 were analyzed using gene chips

and the microarray results were verified with real-time quantitative PCR (qRT-PCR). Based on these results and using bioinformatic analysis

circRNA-IARS was identified as the potential target circRNA in close relation with the metastasis and invasion of pancreatic carcinoma. The expression levels of circRNA-IARS and linar-IARS were detected in 25 pairs of pancreatic carcinoma tissues and adjacent normal tissues using qRT-PCR. The expression of circRNA-IARS was also detected in 115 pancreatic tumor tissues to analyze its relations with the clinicopathological characteristics and prognosis of the patients.

Results

The microarray results showed that 28 circRNA were upregulated in Hs 766T-L2 cells. In the 25 pairs of pancreatic carcinoma and adjacent tissues

the expression of circRNA-IARS was significantly increased in the tumor tissues as compared with the adjacent tissues (

0.05). In patients with pancreatic carcinoma

a high expression of circRNA-IARS in the tumor was found to closely correlate with lymph node involvement

vascular invasion

liver metastasis and TNM stages (

0.05)

and the patients with a high expression level of circRNA-IARS had a significantly shorter postoperative survival than those with a low circRNA-IARS expression (

0.05).

Conclusion

CircRNA-IARS is over-expressed in pancreatic carcinoma

and its expression level is closely related to lymph node invasion

vascular invasion

liver metastasis and TNM stage

and affects the prognosis of the patients.

关键词

Keywords

references

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