Effects of calcitonin gene-related peptide on osteogenic differentiation of human umbilical cord mesenchymal stem cells

Zhen GAO; Qin WU; Junfeng GUO; You LI; Xuedan CHEN; Qiangguo GAO; Gang ZHANG

doi:10.16016/j.1000-5404.201711055

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Effects of calcitonin gene-related peptide on osteogenic differentiation of human umbilical cord mesenchymal stem cells

Basic Medicine | 更新时间：2026-01-09

- Effects of calcitonin gene-related peptide on osteogenic differentiation of human umbilical cord mesenchymal stem cells
- Journal of Third Military Medical University Vol. 40, Issue 6, Pages: 479-486(2018)
- 作者机构：
  
  1.400037 重庆，陆军军医大学(第三军医大学)第二附属医院口腔科
  2.400038 重庆，陆军军医大学(第三军医大学)基础医学院医学遗传学教研室
- 作者简介：
- 基金信息：
  
  the general Program of National Natural Science Foundation of China(81271098)
- DOI：10.16016/j.1000-5404.201711055
  CLC：
- Received：08 November 2017，
  
  Revised：2017-12-19，
  
  Published：30 March 2018
- 稿件说明：
移动端阅览
Zhen GAO, Qin WU, Junfeng GUO, et al. Effects of calcitonin gene-related peptide on osteogenic differentiation of human umbilical cord mesenchymal stem cells[J]. Journal of Third Military Medical University, 2018, 40(6): 479-486.
DOI：

Zhen GAO, Qin WU, Junfeng GUO, et al. Effects of calcitonin gene-related peptide on osteogenic differentiation of human umbilical cord mesenchymal stem cells[J]. Journal of Third Military Medical University, 2018, 40(6): 479-486. DOI： 10.16016/j.1000-5404.201711055.

摘要

目的

探讨降钙素基因相关肽(calcitonin gene related peptide，CGRP)在人脐带间充质干细胞(human umbilical cord mesenchymalstem cells，HUMSCs)向成骨细胞分化过程中的作用及机制。

方法

体外培养HUMSCs细胞，加入0、10

-9

、10

-8

、10

-7

mol/L浓度的CGRP诱导其向成骨细胞分化，分别在第7、9、11、14天时采用茜素红染色检测成骨分化程度，实时定量PCR和Western blot检测ERK、RUNX2、OSTERIX的mRNA和磷酸化的ERK、RUNX2、OSTERIX的蛋白表达。

结果

HUMSCs高表达CD73、CD90、CD105等间充质干细胞表面抗原，但不表达CD14、CD20、CD34、CD45等上皮和造血干细胞的表面抗原，可诱导向成骨细胞分化，随着时间延长矿化结节数量增加(

＜0.05)；随着CGRP浓度的升高，矿化结节减少，ERK、RUNX2、OSTERIX的mRNA和蛋白表达降低(

＜0.05)。

结论

CGRP通过ERK信号通路抑制HUMSCs向成骨细胞的分化，并呈现出浓度和时间依赖性。

Abstract

Objective

To investigate the role and underlying mechanism of calcitonin gene-related peptide (CGRP) in the differentiation of human umbilical cord mesenchymal stem cells (HUMSCs) into osteoblasts.

Methods

Different concentrations (0

-9

-8

and 10

-7

mol/L) of CGRP were added into the culture media of HUMSCs to induce differentiation into osteoblasts. In 7

11 and 14 d after treatment

Alizarin red staining was used to detect the differentiation of osteogenesis. Real-time PCR and Western blotting were employed to detect the mRNA expression levels of ERK

RUNX2 and OSTERIX and protein expression levels of phosphorylation of ERK

RUNX2 and OSTERIX.

Results

HUMSCs with high expression of CD73

CD90

CD105 and other mesenchymal stem cell surface antigens

but not with the expression of CD14

CD20

CD34

CD45 and other epithelial and hematopoietic stem cell surface antigens

were identified to induce differentiation into osteoblasts. The number of mineralized nodules was increased with time elapsing (

＜0.05)

while the number was decreased with the increasing concentrations of CGRP

and the mRNA and protein expression levels of ERK

RUNX2 and OSTERIX were decreased (

＜0.05).

Conclusion

CGRP inhibits the differentiation of HUMSCs into osteoblasts by ERK signaling pathway in a concentration-and time-dependent manner.

关键词

Keywords

references

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