Effect of overexpression of TPX2 gene on apoptosis and invasion in human cervical cancer HeLa cells

Haiping CHANG; Shufang SONG; Jian ZHENG

doi:10.16016/j.1000-5404.201710154

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Effect of overexpression of TPX2 gene on apoptosis and invasion in human cervical cancer HeLa cells

Basic Medicine | 更新时间：2026-01-09

- Effect of overexpression of TPX2 gene on apoptosis and invasion in human cervical cancer HeLa cells
- Journal of Third Military Medical University Vol. 40, Issue 3, Pages: 216-221(2018)
- 作者机构：
  
  010050 呼和浩特，内蒙古医科大学附属医院妇产科
- 作者简介：
- 基金信息：
  
  Supported by the Regional Program of National Natural Science Foundation of China(81360385)
- DOI：10.16016/j.1000-5404.201710154
  CLC： R394.3;R730.23;R737.33
- Received：21 October 2017，
  
  Revised：2017-11-26，
  
  Published：15 February 2018
- 稿件说明：
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Haiping CHANG, Shufang SONG, Jian ZHENG. Effect of overexpression of TPX2 gene on apoptosis and invasion in human cervical cancer HeLa cells[J]. Journal of Third Military Medical University, 2018, 40(3): 216-221.
DOI：

Haiping CHANG, Shufang SONG, Jian ZHENG. Effect of overexpression of TPX2 gene on apoptosis and invasion in human cervical cancer HeLa cells[J]. Journal of Third Military Medical University, 2018, 40(3): 216-221. DOI： 10.16016/j.1000-5404.201710154.

摘要

目的

通过TPX2过表达慢病毒载体在人宫颈癌HeLa细胞过表达TPX2基因，在体外研究TPX2对人宫颈癌HeLa细胞侵袭和凋亡的作用及其相关机制。

方法

构建TPX2过表达慢病毒载体(LV11-TPX2)及阴性对照(LV11-NC)，选取稳定感染过表达慢病毒载体(LV11-TPX2)的HeLa细胞作为过表达组，将稳定感染(LV11-NC)的HeLa细胞作为阴性对照组，未感染病毒的人宫颈癌HeLa细胞作为空白对照组(CON)。采用Transwell基底膜侵袭实验测定各组细胞的侵袭能力，流式细胞术检测各组细胞的凋亡情况，Western blot检测细胞凋亡及侵袭相关蛋白质Bcl-2、Bax、Caspase-3、MMP-9、基质金属蛋白酶抑制剂-1(tissue inhibitor of metalloproteinase-1，TIMP-1)及nm23-H1的表达。

结果

成功构建TPX2过表达慢病毒载体(LV11-TPX2)，可有效过表达TPX2 mRNA及蛋白质。与对照组比较

TPX2过表达组的HeLa细胞凋亡率明显减少(

0.05)，穿过Transwell小室基底膜细胞明显增加(

0.01)。LV11-TPX2感染组HeLa细胞中凋亡相关蛋白Bcl-2的表达明显上调(

0.05)

Caspase-3及Bax的表达明显下调(

0.05)；侵袭相关蛋白质nm23-H1及TIMP-1水平明显下调(

0.05)

MMP-9水平明显上调(

0.05)。

结论

在宫颈癌细胞过表达TPX2基因后，能抑制细胞凋亡，增强其侵袭能力，可能的机制为在宫颈癌细胞过表达TPX2能诱导凋亡和侵袭相关蛋白Caspase-3、Bax、nm23-H1及TIMP-1水平下调，Bcl-2及MMP-9水平上调。

Abstract

Objective

To investigate the effect of targeting protein for Xklp2 (TPX2) on the invasion and apoptosis of human cervical carcinoma HeLa cells by lentiviral vectors carrying TPX2 and to explore its possible mechanism.

Methods

Lentiviral vectors carrying TPX2 (LV11-TPX2) and negative control were constructed respectively. HeLa cells stably infected with LV11-TPX2 served experimental group

the cells infected with LV11-NC as negative control group

and the cells without infection as the blank control group. Cell invasion and apoptosis were determined by Transwell chamber test and flow cytometry

respectively. The expression levels of Bcl-2

Bax

Caspase-3

MMP-9

tissue inhibitor of metalloproteinase-1 (TIMP-1) and nm23-H1 were detected by Western blotting.

Results

The lentiviral vector LV11-TPX2 was successfully constructed

and its infection into HeLa cells resulted in effectively overexpressed TPX2 at mRNA and protein levels. Compared with the control group

the apoptotic rate of LV11-TPX2 group was significantly reduced (

0.05)

and the cells through the Transwell chamber basement membrane was significantly increased (

0.01). Overexpression of TPX2 gene could down-regulate the protein levels of Caspase-3

Bax

nm23-H1 and TIMP-1

and up-regulate the expression of Bcl-2 and MMP-9 in HeLa cells (

0.05).

Conclusion

Overexpression of TPX2 gene inhibits cell apoptosis and promotes invasive ability in cervical carcinoma cells

which may be through down-regulating the expression of Caspase-3

Bax

nm23-H1 and TIMP-1

and up-regulating the expression of Bcl-2 and MMP-9.

关键词

Keywords

references

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