Effects of TGF-β1 on transdifferentiation and extracellular matrix synthesis in L929 cells

Ling WANG; Min LI; Yijun JIA; Yusi QIN; Guiyong PENG

doi:10.16016/j.1000-5404.201710068

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Effects of TGF-β1 on transdifferentiation and extracellular matrix synthesis in L929 cells

Basic Medicine | 更新时间：2026-01-09

- Effects of TGF-β1 on transdifferentiation and extracellular matrix synthesis in L929 cells
- Journal of Third Military Medical University Vol. 40, Issue 5, Pages: 367-371, 379(2018)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院全军消化病研究所
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81470907)
- DOI：10.16016/j.1000-5404.201710068
  CLC： R322.43;R329.28;R34
- Received：12 October 2017，
  
  Revised：2017-12-11，
  
  Published：15 March 2018
- 稿件说明：
移动端阅览
Ling WANG, Min LI, Yijun JIA, et al. Effects of TGF-β1 on transdifferentiation and extracellular matrix synthesis in L929 cells[J]. Journal of Third Military Medical University, 2018, 40(5): 367-371, 379.
DOI：

Ling WANG, Min LI, Yijun JIA, et al. Effects of TGF-β1 on transdifferentiation and extracellular matrix synthesis in L929 cells[J]. Journal of Third Military Medical University, 2018, 40(5): 367-371, 379. DOI： 10.16016/j.1000-5404.201710068.

摘要

目的

探讨转化生长因子β1(transforming growth factor-β1，TGF-β1)对L929细胞增殖的影响，为体外研究预防食管内镜黏膜下剥离术(endoscopic submucosal dissection，ESD)术后狭窄提供理论基础。

方法

实验分为两组，①空白对照组：L929细胞不做处理；②TGF-β1组，用10 ng/mL TGF-β1持续刺激L929细胞，依据刺激时间不同又分为24、48、72、96、120 h 5个亚组。观察各组对细胞增殖的影响。用CCK-8法检测细胞增殖，qPCR检测α-SMA及Ⅰ型前胶原、Ⅲ型前胶原、MMP1、TIMP1 mRNA表达，Western blot检测α-SMA、MMP1、TIMP1蛋白水平，ELISA检测Ⅰ型前胶原、Ⅲ型前胶原合成。

结果

与空白对照组比较，TGF-β1组细胞增殖能力显著增高(

0.05)，TGF-β1组α-SMA、Ⅰ型前胶原、Ⅲ型前胶原、MMP1、TIMP1 mRNA表达均显著升高(

0.05)；TGF-β1刺激24 h组Ⅰ型前胶原合成增加(

0.05)，TGF-β1组Ⅲ型前胶原合成增加(

0.05)；TGF-β1组MMP1、TIMP1蛋白表达增加(

0.05)。

结论

TGF-β1能促进L929细胞增殖，并诱导L929细胞向肌成纤维细胞转分化，同时促进Ⅰ型前胶原、Ⅲ型前胶原以及MMP1、TIMP1合成。

Abstract

Objective

To investigate the effect of TGF-β1 on the growth of L929 cells in order to found the basis for theoretical study on prevention of esophageal stricture after endoscopic submucosal dissection.

Methods

Experiments had two sets: a control group without any treatments and a TGF-β1 treatment group in which cells were treated with 10 ng/mL TGF-β1. According to the time of stimulation by TGF-β1

subgroups were set as 24

96 and 120 h treatment groups

the cell proliferation were measured with CCK-8 assay in every group of cells. qPCR was used to detect the mRNA expression of α -SMA and procollagen type Ⅰ (Col Ⅰ) and Ⅲ (Col Ⅲ)

matrix metalloproteinase 1 (MMP1)

tissue inhibitor of metalloprotein 1 (TIMP1). Western blotting was employed for protein levels of α -SMA

MMP1 and TIMP1. ELISA was adopted to measure the synthesis of Col Ⅰ and Col Ⅲ.

Results

Compared with the blank control cells

TGF-β1 treatment significantly promote cell proliferation (

0.05)

and enhanced the mRNA levels of α-SMA

Col Ⅰ

Col Ⅲ

MMP1 and TIMP1 in L929 cells (

0.05). TGF-β1 stimulation for 24 h increased the syntheses of Col Ⅰ (

0.05) and Col Ⅲ (

0.05)

and protein level of MMP1. The treatment for 96 and 120 h also enhanced the protein level of TIMP1 (

0.05).

Conclusion

TGF-β1 can promote the proliferation of L929 cells

induce the transformation of L929 cells into myofibroblasts

and improve the syntheses of Col Ⅰ

Col Ⅲ

MMP1 and TIMP1.

关键词

Keywords

references

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