Oxymatrine inhibits viral replication in HBV transgenic mice by regulating T lymphocyte subsets

Jingjing XIE; Xuefeng YANG

doi:10.16016/j.1000-5404.201709193

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Oxymatrine inhibits viral replication in HBV transgenic mice by regulating T lymphocyte subsets

Basic Medicine | 更新时间：2026-01-09

- Oxymatrine inhibits viral replication in HBV transgenic mice by regulating T lymphocyte subsets
- Journal of Third Military Medical University Vol. 40, Issue 5, Pages: 415-419(2018)
- 作者机构：
  
  1.421002 湖南衡阳，南华大学附属南华医院感染科
  2.421002 湖南衡阳，南华大学附属南华医院消化内科
- 作者简介：
- 基金信息：
  
  the Key Project of Natural Science Foundation of Hunan Province(12JJ2058)
- DOI：10.16016/j.1000-5404.201709193
  CLC： R285.5;R373.21;R967
- Received：26 September 2017，
  
  Revised：2017-12-06，
  
  Published：15 March 2018
- 稿件说明：
移动端阅览
Jingjing XIE, Xuefeng YANG. Oxymatrine inhibits viral replication in HBV transgenic mice by regulating T lymphocyte subsets[J]. Journal of Third Military Medical University, 2018, 40(5): 415-419.
DOI：

Jingjing XIE, Xuefeng YANG. Oxymatrine inhibits viral replication in HBV transgenic mice by regulating T lymphocyte subsets[J]. Journal of Third Military Medical University, 2018, 40(5): 415-419. DOI： 10.16016/j.1000-5404.201709193.

摘要

目的

观察氧化苦参碱对HBV转基因小鼠病毒复制的作用，并探讨其是否与调节T淋巴细胞亚群有关。

方法

选取SPF级HBV转基因雄性小鼠36只，按随机数字表法分为模型组、氧化苦参碱组及拉米夫定组，每组12只，另取12只非转基因BALB/c雄性小鼠作为对照组。各组分别给予相应的药物治疗。观察各组小鼠肝脏组织病理变化，并检测血清及肝脏乙肝表面抗原(HBsAg)、乙型肝炎病毒HBV-DNA水平、血液T淋巴细胞亚群水平以及血清细胞因子水平。

结果

治疗后，与模型组比较，氧化苦参碱组与拉米夫定组血清及肝组织HBsAg、HBV-DNA水平较低(

0.05)，而氧化苦参碱组与拉米夫定组血清及肝组织HBsAg、HBV-DNA水平差异无统计学意义(

0.05)。与模型组比较，氧化苦参碱组与拉米夫定组血液CD3

、CD4

T淋巴细胞、CD4

/CD8

水平较高，且氧化苦参碱组＞拉米夫定组；CD8

T淋巴细胞水平较低，且氧化苦参碱组＜拉米夫定组(

0.05)；氧化苦参碱组与拉米夫定组血清干扰素-γ(IFN-γ)、白细胞介素-2(IL-2)水平较高，且氧化苦参碱组＞拉米夫定组；白细胞介素-4(IL-4)水平较低，且氧化苦参碱组＜拉米夫定组，差异均有统计学意义(

0.05)。

结论

氧化苦参碱能有效抑制HBV转基因小鼠血清及肝组织HBsAg、HBV-DNA水平，该作用可能与调节T淋巴细胞亚群水平及Th1/Th2细胞因子水平、改善免疫功能有关。

Abstract

Objective

To determine the inhibitory effect of oxymatrine on viral replication in HBV transgenic mice

and investigate whether this effect is associated with regulating T lymphocyte subsets.

Methods

Thirty-six SPF male HBV transgenic mice were randomly divided into model group

oxymatrine group and lamivudine group

with 12 rats in each group. Another 12 non-transgenic BALB/c mice served as control group. All rats were given corresponding drug treatment. Pathological changes of liver tissue were observed in each group

and the levels of hepatitis B surface antigen (HBsAg)

hepatitis B virus deoxyribonucleic acid (HBV-DNA) in serum and liver

serum T lymphocyte subsets

and serum cytokine levels were detected.

Results

After treatment

the HBsAg and HBV-DNA levels in serum and liver were significantly lower in the oxymatrine group and lamivudine group than the model group (

0.05)

but there was no significant difference in t

he serum and liver levels between the oxymatrine group and lamivudine group (

0.05). Compared with the model control group

the proportions of CD3

and CD4

T lymphocytes and the ratio of CD4

/CD8

were obviously higher

while the proportion of CD8

T lymphocytes was quite lower in the oxymatrine group and lamivudine group

but the oxymatrine group had better above values than the lamivudine group (

0.05). What's more

the serum levels of interferon-γ (IFN-γ) and interleukin-2 (IL-2) were higher while that of interleukin-4 (IL-4) was lower in the oxymatrine group and lamivudine group than the model group

and the oxymatrine group also had better values (

0.05).

Conclusion

Oxymatrine can effectively inhibit the levels of HBsAg and HBV-DNA in serum and liver tissue of HBV transgenic mice

which may be mediated by regulating the proportions of T lymphocyte subsets and the levels of Th1/Th2 cytokines

and improving the immune function.

关键词

Keywords

references

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