Drug resistance and epidemiological characteristics of <italic style="font-style: italic">Acinetobacter baumannii</italic> isolated from respiratory department of our hospital, Chongqing

Fujia YANG; Wei FENG; Fengjun SUN

doi:10.16016/j.1000-5404.201709112

您当前的位置：

首页 >

文章列表页 >

Drug resistance and epidemiological characteristics of Acinetobacter baumannii isolated from respiratory department of our hospital, Chongqing

Clinical Medicine | 更新时间：2026-01-09

- Drug resistance and epidemiological characteristics of Acinetobacter baumannii isolated from respiratory department of our hospital, Chongqing
- Journal of Third Military Medical University Vol. 40, Issue 3, Pages: 259-263(2018)
- 作者机构：
  
  1.100069 北京，首都医科大学口腔医学系
  2.400038 重庆，陆军军医大学(第三军医大学)第一附属医院药学部
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201709112
  CLC： R181.24;R378.7;R915
- Received：15 September 2017，
  
  Revised：2017-10-20，
  
  Published：15 February 2018
- 稿件说明：
移动端阅览
Fujia YANG, Wei FENG, Fengjun SUN. Drug resistance and epidemiological characteristics of Acinetobacter baumannii isolated from respiratory department of our hospital, Chongqing[J]. Journal of Third Military Medical University, 2018, 40(3): 259-263.
DOI：

Fujia YANG, Wei FENG, Fengjun SUN. Drug resistance and epidemiological characteristics of Acinetobacter baumannii isolated from respiratory department of our hospital, Chongqing[J]. Journal of Third Military Medical University, 2018, 40(3): 259-263. DOI： 10.16016/j.1000-5404.201709112.

摘要

目的

探讨呼吸科鲍曼不动杆菌耐药及流行病学特征。

方法

收集2015年1-12月陆军军医大学第一附属医院呼吸科院内感染患者各类临床标本分离的69株鲍曼不动杆菌

通过医院电子病历系统获得患者临床特征

采用琼脂平板倍比稀释法检测细菌最低抑菌浓度

PCR扩增碳青霉烯酶耐药基因

脉冲场凝胶电泳对鲍曼不动杆菌进行基因分型。

结果

呼吸机使用、抗菌药物使用及住院时间是患者感染鲍曼不动杆菌的危险因素(

0.001)。除米诺环素外

鲍曼不动杆菌对其他抗菌药物均具有较高的耐药性。所有菌株含有OXA-51基因

51株携带OXA-23基因

13株携带VIM基因

2株携带IMP-4基因。69株鲍曼不动杆菌共分为9个型和19个亚型

其中D型和H型最为普遍流行。

结论

我院呼吸科鲍曼不动杆菌耐药率较高

具有耐药基因水平传播和小规模暴发流行的特点。

Abstract

Objective

To explore the drug resistance and epidemiological characteristics of isolated

Acinetobacter baumannii

(

A. baumannii

Methods

A total of 69 stains of

A. baumannii

were isolated from the different clinical specimens of nosocomial infectious patients hospitalized in the respiratory department of the First Affiliated Hospital of Army Medical University from January 2015 to December 2015. The clinical data of these patients were obtained from the electronic medical record system of the hospital. The antimicrobial susceptibility was evaluated by agar disk dilution. The carbapenemase resistance genes were amplified by PCR

and the gene types were analyzed by the pulsed-field gel electrophoresis (PFGE).

Results

Mechanical ventilation

prior antibiotics and long hospital stay were the risk factors for

A. baumannii

infection (

0.001). Except for minocycline

our isolated strains showed high drug resistance rates to the other antibiotics. All the isolates carried OXA-51 gene

and 51 isolates had OXA-23 gene. VIM gene presented in 13 isolates

and IMP gene only existed in 2 isolates. The PFGE results showed that the 69 isolates had 9 different genotypes and 19 subtypes

and D and E genotypes were the most popular.

Conclusion

Our isolated

A. baumannii

stains have quite higher resistance rate

and are characterized by horizontal spread of resistance genes and small-scale epidemic outbreak.

关键词

Keywords

references

HOWARD A, OƊONOGHUE M, FEENEY A, et al . Acinetobacter baumannii : an emerging opportunistic pathogen[J ] . Virulence, 2012, 3(3): 243-250.DOI:10.4161/viru.19700..

PEREZ F, HUJER A M, HUJER K M, et al . Global challenge of multidrug-resistant Acinetobacter baumannii [J ] . Antimicrob Agents Chemother, 2007, 51(10): 3471-3484. DOI:10.1128/AAC.01464-06..

施腾飞, 陈惠瑜, 刘银环, 等.福州地区碳青霉烯类耐药鲍曼不动杆菌碳青霉烯酶基因型研究[J].中国微生态学杂志, 2017, 29(2): 155-158. DOI:10.13381/j.cnki.cjm.201702008.

SHI T F, CHEN H Y, LIU Y H, et al . The genotypes of carbapenemases in carbapenem-resistant Acinetobacter baumannii in fuzhou area[J ] . Chin J Microecol, 2017, 29(2): 155-158. DOI:10.13381/j.cnki.cjm.201702008..

SHETE V B, GHADAGE D P, MULEY V A, et al . Multi-drug resistant Acinetobacter ventilator-associated pneumonia[J ] . Lung India, 2010, 27(4): 217-220. DOI:10. 4103/0970-2113.71952..

孙凤军, 戴晓天, 冯伟, 等. 2003-2011年某院呼吸科病原菌的分布及耐药性分析[J].重庆医学, 2015, 44(14): 1931-1933. DOI:10.3969/j.issn.1671-8348.2015.14.022.

SUN F J, DAI X T, FENG W, et al . Analysis on distribution and drug resistance of pathogens in respiratory department from 2003 to 2011[J ] . Chongqing Med, 2015, 44(14): 1931-1933. DOI:10.3969/j.issn.1671-8348.2015.14.022.

GONG Y, SHEN X, HUANG G, et al . Epidemiology and resistance features of Acinetobacter baumannii isolates from the ward environment and patients in the burn ICU of a Chinese hospital[J ] . J Microbiol, 2016, 54(8): 551-558. DOI:10.1007/s12275-016-6146-0..

SUN F, OU Q, WANG Q, et al . The resistance and transmission mechanism of Acinetobacter baumannii isolates in a tertiary care hospital, China[J ] . J Chemother, 2016, 28(6): 476-481. DOI:10.1080/1120009X.2016.1139335..

DAI X T, SUN F J, CHEN Z H, et al . The epidemiology and resistance mechanisms of Acinetobacter baumannii isolates from the respiratory department ICU of a hospital in China[J ] . Microb Drug Resist, 2014, 20(6): 618-622. DOI:10.1089/mdr.2014.0005..

DIJKSHOORN L, NEMEC A, SEIFERT H. An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii [J ] . Nat Rev Microbiol, 2007, 5(12): 939-951. DOI:10.1038/nrmicro1789.

RAMOS T, DEDESKO S, SIEGEL J A, et al . Spatial and temporal variations in indoor environmental conditions, human occupancy, and operational characteristics in a new hospital building[J ] . PLoS ONE, 2015, 10(3): e0118207. DOI:10.1371/journal.pone.0118207.

LUO T L, RICKARD A H, SRINIVASAN U, et al . Association of blaOXA-23 and bap with the persistence of Acinetobacter baumannii within a major healthcare system[J ] . Front Microbiol, 2015, 6:182.DOI:10.3389/fmicb.2015.00182..

JI Y J, PARK M S, SONG E K, et al . Risk factors for multi-drug resistant Acinetobacter baumannii bacteremia in patients with colonization in the intensive care unit[J ] . BMC Infectious Dis, 2010, 10(1):228. DOI:10.1186/1471-2334-10-228..

马明远, 徐杰, 于娜, 等.综合ICU内鲍曼不动杆菌的耐药性和相关因素分析[J].中华危重病急救医学, 2013, 25(11):686-689. DOI:10.3760/cma.j.issn.2095-4352.2013.11.012.

MA M Y, XU J, YU N, et al . Analysis of drug resistance of Acinetobacter baumannii and its related factors in ICU[J ] . Zhonghua Wei Zhong Bing Ji Jiu Yi Xue, 2013, 25(11): 686-689. DOI:10.3760/cma.j.issn.2095-4352.2013.11.012..

ZHENG W, YUAN S, LI L. Analysis of hospital departmental distribution and antibiotic susceptibility of Acinetobacter isolated from sputum samples[J ] . Am J Infect Control, 2013, 41(8): e73-e76. DOI:10.1016/j.ajic.2012.11.004.

YAN Z Q, SHEN D X, CAO J R, et al . Susceptibility patterns and molecular epidemiology of multidrug-resistant Acinetobacter baumannii strains from three military hospitals in China[J ] . Int J Antimicrob Agents, 2010, 35(3): 269-273. DOI:10.1016/j.ijantimicag.2009.10.016..

PARK G C, CHOI J A, JA NG S J, et al . In vitro interactions of antibiotic combinations of colistin, tigecycline, and doripenem against extensively drug-resistant and multidrug-resistant Acinetobacter baumannii [J ] . Ann Lab Med, 2016, 36(2): 124-130. DOI:10.3343/alm.2016.36.2.124..

MINH V L, NHU N T K, PHAT V V, et al . In vitro activity of colistin in antimicrobial combination against carbapenem-resistant Acinetobacter baumannii isolated from patients with ventilator-associated pneumonia in Vietnam[J ] . J Med Microbiol, 2015, 64(10): 1162-1169. DOI:10.1099/jmm.0.000137..

POGUE J M, MANN T, BARBER K E, et al . Carbapenem-resistant Acinetobacter baumannii : epidemiology, surveillance and management[J ] . Expert Rev Anti Infect Ther, 2013, 11(4): 383-393. DOI:10.1586/eri.13.14..

OPAZO A, DOMÍNGUEZ M, BELLO H, et al . OXA-type carbapenemases in Acinetobacter baumannii in South America[J ] . J Infect Dev Ctries, 2012, 6(4): 311-316. DOI:10.3855/jidc.2310..

王振, 黄文祥, 辛小娟, 等.泛耐药鲍曼不动杆菌医院感染流行病学研究[J].第三军医大学学报, 2011, 33(21): 2244-2248.

WANG Z, HUANG W X, XIN X J, et al . Epidemiological study on nosocomial i nfection of Acinetobacter baumannii [J ] . J Third Mil Med Univ, 2011, 33(21): 2244-2248..

CHEN H, YUEHUA X, SHEN W, et al . Epidemiology and resistance mechanisms to imipenem in Klebsiella pneumoniae: a multicenter study[J ] . Mol Med Rep, 2013, 7(1): 21-25. DOI:10.3892/mmr.2012.1155.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Achievements and challenges: Key priorities for prevention and control in the elderly amid the declining disease burden of benign prostatic hyperplasia in China

Overexpression of Mdm2 induces crizotinib resistance in targeted therapy for ALK-positive NSCLC

Epidemiological characteristics and trends of primary intussusception among children in Wuhan from 2016 to 2022

Comparison of different adjuvants for immune efficacy of recombinant PKF protein of Acinetobacter

Resistance rate and mechanism of ureaplasma urealyticum to fluoroquinolones in infertile patients

Related Author

ZHANG Bowen

LIU Yunlong

DING Mengdi

ZHAO Ying

ZHAO Yu

LIU Xiaomei

FANG Kai

LI Meng

Related Institution

Department of Urology， the Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine

Department of Urology， Xiyuan Hospital， China Academy of Chinese Medical Sciences

Department of Andrology， the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine， National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion

Center for Clinical Pharmacology

Department of Respiratory and Critical Care Medicine, General Hospital of Eastern Theater Command

⁰

Drug resistance and epidemiological characteristics of Acinetobacter baumannii isolated from respiratory department of our hospital, Chongqing

DOI：10.16016/j.1000-5404.201709112

摘要

Abstract

关键词

Keywords

references