Glucose metabolic alteration in oral carcinoma-associated fibroblasts

Wanrong TANG; Ying MA; Jie YU; Lihua LI; Ying LIU

doi:10.16016/j.1000-5404.201709090

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Glucose metabolic alteration in oral carcinoma-associated fibroblasts

Basic Medicine | 更新时间：2026-01-09

- Glucose metabolic alteration in oral carcinoma-associated fibroblasts
- Journal of Third Military Medical University Vol. 40, Issue 1, Pages: 53-57(2018)
- 作者机构：
  
  637000 四川南充, 川北医学院口腔医学系
- 作者简介：
- 基金信息：
  
  the Doctoral Program Foundation of North Sichuan Medical College(CBY13-QD-07);the Key Project of Scientific Research Development of North Sichuan Medical College(CBY15-A-ZD09)
- DOI：10.16016/j.1000-5404.201709090
  CLC： Q591.4;R730.23;R739.8
- Received：12 September 2017，
  
  Revised：2017-11-01，
  
  Published：15 January 2018
- 稿件说明：
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Wanrong TANG, Ying MA, Jie YU, et al. Glucose metabolic alteration in oral carcinoma-associated fibroblasts[J]. Journal of Third Military Medical University, 2018, 40(1): 53-57.
DOI：

Wanrong TANG, Ying MA, Jie YU, et al. Glucose metabolic alteration in oral carcinoma-associated fibroblasts[J]. Journal of Third Military Medical University, 2018, 40(1): 53-57. DOI： 10.16016/j.1000-5404.201709090.

摘要

目的

通过对口腔鳞癌相关成纤维细胞(carcinoma-associate fibroblasts

CAFs)糖代谢相关指标的检测

探讨肿瘤微环境CAFs糖代谢的可能方式并分析其作用和意义。

方法

培养CAFs和癌旁正常成纤维细胞(normal fibroblasts

NFs)

詹纳斯绿B染色观察线粒体的数量及分布

JC-1荧光染色观察线粒体膜电位的改变。培养1、2、3、4 d

通过定磷法用可见分光光度计检测细胞中ATP的含量

葡萄糖氧化酶-过氧化物酶法检测细胞摄取葡萄糖的能力

乳酸酶法检测细胞分泌乳酸情况。

结果

与NFs组相比

CAFs线粒体詹纳斯绿B和JC-1荧光染色强度明显下降

显示CAFs线粒体活性下降。随着培养时间的延长

CAFs较NFs组摄取葡萄糖增多

ATP产能却减少

分泌乳酸增加

差异均有统计学意义(

0.05);以培养第4天差异更明显

CAFs和NFs组培养基葡萄糖浓度分别为(10.85±1.42)、(16.04±1.09)mmol/L

产生ATP浓度分别为(42.99±4.64)、(57.37±2.75)μmol/g

产生乳酸浓度分别为(12.1±0.81)、(7.06±0.95)mmol/L。

结论

口腔鳞癌CAFs细胞糖代谢方式发生了改变

以糖酵解为主。

Abstract

Objective

To explore the possible ways of glucose metabolism in carcinoma-associated fibroblasts (CAFs) by detecting the related indexes of metabolism

and analyze its function and significance in tumor-stroma interplay.

Methods

After the CAFs and paracancerous normal fibroblasts (NFs) were cultured

the amount and distribution of mitochondria were observed by Janus green B staining. The mitochondrial membrane potential was tested with specific fluorescent dye JC-1 staining. The phosphomolybdic acid colorimetry was used to measure the ATP content of the cells. Glucose oxidase-peroxidase reagent was used to detect the intracellular glucose level. The change of lactate concentration in 2 groups of cells was detected by lactic acid assay kit.

Results

Compared with the NFs group

the mitochondrial activity was lower in the CAFs group

according to the results of Janus green B staining and JC-1 fluorescence staining. As the time elapsed

the ability of glucose-uptaking and lactate secretion were increased in CAFs group

while ATP production was declined

with significant difference when compared with the NFs group (

0.05). At the fouth day

glucose concentration were 10.85±1.42 mmol/L in the CAFs group and 16.04±1.09 mmol/L in the NFs group

while

ATP concentration was 42.99±4.64 and 57.37±2.75 μmol/g

respectively

and lactic acid concentration were 12.1±0.81 and 7.06±0.95 mmol/L

separately.

Conclusion

Oral squamous CAFs have glucose metabolic alteration

mainly through glycolysis.

关键词

Keywords

references

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