Enhanced Cerenkov luminescence imaging
in vitro and in tumor-bearing mice using tetracycline, fluorescein isothiocyanate, and fluorescein sodium- Journal of Third Military Medical University Vol. 39, Issue 22, Pages: 2182-2188(2017)
- 作者机构：
100039 北京，武警总医院消化内科
- 作者简介：
- 基金信息：the General Program of National Natural Science Foundation of China(81471700)
DOI：10.16016/j.1000-5404.201709064
CLC： R312;R817.4;R981.1Received：10 September 2017，
Revised：2017-09-20，
Published：30 November 2017
- 稿件说明：
移动端阅览
Sheng ZHENG, Yawei QU, Jiali ZHA, et al. Enhanced Cerenkov luminescence imaging
in vitro and in tumor-bearing mice using tetracycline, fluorescein isothiocyanate, and fluorescein sodium[J]. Journal of Third Military Medical University, 2017, 39(22): 2182-2188.Sheng ZHENG, Yawei QU, Jiali ZHA, et al. Enhanced Cerenkov luminescence imaging
in vitro and in tumor-bearing mice using tetracycline, fluorescein isothiocyanate, and fluorescein sodium[J]. Journal of Third Military Medical University, 2017, 39(22): 2182-2188. DOI： 10.16016/j.1000-5404.201709064.
摘要
目的
2
探究四环素、荧光素钠、异硫氰酸荧光素(fluorescein isothiocyanate，FITC)这3种临床可用的荧光材料对契伦科夫光学信号增强的效果及最佳应用方案。
方法
2
将不同浓度的四环素溶液(100、50、10、5、1、0.5、0.1、0.05、0.01 mmol/L)、FITC溶液(5、4、3、2.5、1.5、1、0.5、0.25 mmol/L)和荧光素钠溶液(100、50、10、5、2、1、0.2、0.1 mmol/L)分别与100 μCi的
18
F-FDG混合后进行契伦科夫光学成像(Cerenkov luminescence imaging，CLI)，观察契伦科夫光学信号的增强效果，并勾画感兴趣区域(region of interest，ROI)，对光学信号强度进行定量分析，确定最佳信号增强效果的浓度，并对信号最强的板孔以20 nm为间隔进行500~800 nm范围的光谱分离成像。同时进行在体实验验证荧光素钠及FITC对契伦科夫光的增强效果。
结果
2
不同浓度的四环素溶液对光学信号无明显影响。FITC及荧光素钠均能显著提高契伦科夫光学信号强度。当FITC及荧光素钠浓度分别为1或2 mmol/L时产生的光学信号强度最强，为单独的
18
F-FDG的3.2倍及5倍。光谱分析表明2 mmol/L的荧光素钠能将
18
F-FDG产生的契伦科夫光(cerenkov luminescence，CL)光红移至540 nm附近。荷瘤鼠在体实验表明注射FITC或荧光素钠后能使肿瘤部位的CL信号强度提高2倍或3.9倍。
结论
2
FITC、荧光素钠均能通过契伦科夫光的激发效应来增强契伦科夫光学信号强度，提高组织穿透力。
Abstract
Objective
2
To compare the performance of 3 clinically available fluorescent materials
namely tetracycline
fluorescein sodium
and fluorescein isothiocyanate (FITC)
in enhancing Cerenkov luminescence imaging (CLI) signal and determine the best strategy for enhanced CLI.
Methods
2
In vitro
CLI was performed using
18
F-FDG (100 μCi) mixed with different concentrations of tetracycline (100
50
10
5
1
0.5
0.1
0.05
and 0.01 mmol/L)
fluorescein sodium (5
4
3
2.5
1.5
1
0.5
and 0.25 mmol/L)
or FITC (100
50
10
5
2
1
0.2
and 0.1 mmol/L)
and the enhancement of Cerenkov radiance (CR) intensity was assessed. The optical signal intensity was quantified by region of interest (ROI) analysis to determine the optimal concentration of the agents for signal enhancement. The emission spectra from the wells with the strongest signals were analyzed at a 20-nm interval within the spectrum range of 500 to 800 nm. The effects of fluorescein sodium and FITC in enhancing CR intensity were also verified by
in vivo
imaging in a tumor-bearing nude mouse model.
Results
2
The results of
in vitro
CLI showed that tetracycline failed to enhance the CR of
18
F-FDG
while both fluorescein sodium and FITC significantly enhanced the intensity of Cerenkov signals. Fluorescein sodium at 2 mmol/L and FITC at 1 mmol/L resulted in the strongest enhancement of CR intensity by 3.2 and 5 folds
respectively. Spectrum analysis confirmed that fluorescein sodium caused a red shift of Cerenkov spectrum peak to 540 nm.
In vivo
CLI of the tumor-bearing mice showed that injection of fluorescein sodium and FITC increased the CR intensity at the tumor site by 3.9 and 2 folds
respectively.
Conclusion
2
FITC and fluorescein sodium can both enhance the optical signal intensity and improve the tissue penetration of CLI by excitation of CR.
关键词
Keywords
references
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