Resveratrol improves lipid droplets lipolysis <italic style="font-style: italic">via</italic> up-regulating PKA-PLIN2 pathway in HepG2 cells

Xikun YE; Yu QIN; Li RAN; Rui ZHOU; Long YI; Mantian MI

doi:10.16016/j.1000-5404.201709044

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Resveratrol improves lipid droplets lipolysis via up-regulating PKA-PLIN2 pathway in HepG2 cells

Basic Medicine | 更新时间：2026-01-09

- Resveratrol improves lipid droplets lipolysis via up-regulating PKA-PLIN2 pathway in HepG2 cells
- Journal of Third Military Medical University Vol. 39, Issue 24, Pages: 2345-2351(2017)
- 作者机构：
  
  400038 重庆，第三军医大学军事预防医学院营养与食品卫生学教研室，营养与食品安全研究中心，重庆市医学营养研究中心，重庆市营养与食品安全重点实验室
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81673154)
- DOI：10.16016/j.1000-5404.201709044
  CLC： R282.71; R285.1; R575.5
- Received：07 September 2017，
  
  Revised：2017-09-28，
  
  Published：30 December 2017
- 稿件说明：
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Xikun YE, Yu QIN, Li RAN, et al. Resveratrol improves lipid droplets lipolysis via up-regulating PKA-PLIN2 pathway in HepG2 cells[J]. Journal of Third Military Medical University, 2017, 39(24): 2345-2351.
DOI：

Xikun YE, Yu QIN, Li RAN, et al. Resveratrol improves lipid droplets lipolysis via up-regulating PKA-PLIN2 pathway in HepG2 cells[J]. Journal of Third Military Medical University, 2017, 39(24): 2345-2351. DOI： 10.16016/j.1000-5404.201709044.

摘要

目的

明确围脂滴蛋白2(perilipinin-2，PLIN2)在白藜芦醇(resveratrol，RSV)改善脂肪变性HepG2细胞脂滴代谢中的作用机制。

方法

0.2 mmol/L棕榈酸处理HepG2细胞24 h，建立肝细胞脂肪变性模型。使用0、10、20、40、80 μmol/L RSV及转染PLIN2 siRNA或PKI过表达质粒后，再用40 μmol/L RSV干预24 h，油红O染色检测脂滴形成情况，酶法检测细胞内甘油三酯以及细胞外游离甘油含量，Western blot检测PLIN2蛋白表达，实时定量PCR检测PLIN2 mRNA表达。

结果

RSV可以降低脂肪变性HepG2细胞脂滴蓄积程度，促进脂滴的脂解。RSV可以通过促进磷酸化增强PKA的活性，并呈浓度依赖性地上调PLIN2 mRNA和蛋白表达水平(

＜0.05)。抑制PKA活性或下调PLIN2表达后，RSV促进脂肪变性HepG2细胞脂解的作用显著减弱(

＜0.05)。

结论

RSV通过激活PKA-PLIN2通路促进HepG2细胞脂滴脂解过程，从而可能改善非酒精性脂肪性肝病。

Abstract

Objective

Elucidate the role of perilipinin-2 (PLIN2) in RSV improving lipid droplet metabolism in liver steatosis in HepG2 cells.

Methods

HepG2 cells were incubated with 0.2 mmol/L of palmitic acid (PA) for 24 h to set up a hepatic steatosis model. The cells were then treated with or without a series of concentrations (10

40 and 80 μmol/L) of RSV

or transfected with PLIN2 siRNA or PKI overexpression plasmid

40 μmol/L RSV was added for another 24 h. Hepatic lipid accumulation was assayed by Oil Red O staining. Contents of triglyceride in cells and free glycerin glycerol in culture medium were detected by Enzyme method. The protein and mRNA levels of PLIN2 were measured by Western blot assay and real-time quantitative PCR

respectively.

Results

RSV reduced the lipid droplet accumulation and increased the lipolysis in PA-induced HepG2 cells. Additionally

RSV increased the activity of PKA by phosphorylation and dose-dependently raised the mRNA and protein levels of PLIN2(

0.05). The promoting effect of RSV on the lipolysis was significantly reduced by the inhibition of PKA activity and the down-regulation of PLIN2 (

0.05).

Conclusion

RSV promotes lipid droplet lipolysis by activating PKA-PLIN2 pathway in HepG2 cells

and may attenuate non-alcoholic fatty liver disease.

关键词

Keywords

references

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Resveratrol improves lipid droplets lipolysis via up-regulating PKA-PLIN2 pathway in HepG2 cells

DOI：10.16016/j.1000-5404.201709044

摘要

Abstract

关键词

Keywords

references