Role and mechanism of adrenomedullin expression in <italic style="font-style: italic">Helicobacter pylori</italic> infection

Hui KONG; Yuan ZHUANG; Fangyuan MAO; Liusheng PENG; Yongsheng TENG; Shugang LIU; Yipin LYU; Gang GUO

doi:10.16016/j.1000-5404.201709041

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Role and mechanism of adrenomedullin expression in Helicobacter pylori infection

Basic Medicine | 更新时间：2026-01-09

- Role and mechanism of adrenomedullin expression in Helicobacter pylori infection
- Journal of Third Military Medical University Vol. 40, Issue 3, Pages: 183-189(2018)
- 作者机构：
  
  重庆，陆军军医大学(第三军医大学)药学与检验医学系微生物与生化药学教研室
- 作者简介：
- 基金信息：
  
  Supported by the General Program of National Natural Science Foundation of China(81670510)
- DOI：10.16016/j.1000-5404.201709041
  CLC： R377;R392.3;R573.02
- Received：06 September 2017，
  
  Revised：2017-11-14，
  
  Published：15 February 2018
- 稿件说明：
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Hui KONG, Yuan ZHUANG, Fangyuan MAO, et al. Role and mechanism of adrenomedullin expression in Helicobacter pylori infection[J]. Journal of Third Military Medical University, 2018, 40(3): 183-189.
DOI：

Hui KONG, Yuan ZHUANG, Fangyuan MAO, et al. Role and mechanism of adrenomedullin expression in Helicobacter pylori infection[J]. Journal of Third Military Medical University, 2018, 40(3): 183-189. DOI： 10.16016/j.1000-5404.201709041.

摘要

目的

探讨肾上腺髓质素(adrenomedullin

ADM)在幽门螺杆菌感染中的表达调控及其对CD3

T细胞增殖的影响。

方法

建立幽门螺杆菌(

Helicobacter pylori

H.pylori

)感染C57小鼠及胃上皮细胞模型，运用定量PCR、免疫组织化学染色和Western blot检测ADM的表达；并在细胞模型中探讨

H.pylori

感染诱导ADM上调的机制；利用ADM刺激CD3

T细胞，检测其对T细胞增殖的影响。

结果

H.pylori

感染可诱导胃上皮细胞AGS和HGC-27上调ADM的表达，并具有时间和感染菌量依赖性(

0.05)；PI3K/AKT信号通路阻断可显著抑制

H.pylori

感染诱导的ADM上调表达(

0.05)；相对于未感染组，

H.pylori

感染小鼠胃组织中的ADM水平也显著增高；而相对于野生型全毒株，敲除cagA基因后，

H.pylori

感染诱导ADM上调的能力则显著下降(

0.05)；ADM能够以剂量依赖的方式诱导CD3

T细胞的增殖并促进IFN-γ的表达(

0.05)。

结论

幽门螺杆菌感染可经PI3K/AKT信号诱导肾上腺髓质素的表达上调，后者则可能通过影响CD3

T细胞的增殖和分化而发挥免疫调节作用。

Abstract

Objective

To explore the expression regulation of adrenomedullin (ADM) in

Helicobacter pylori

(

H.pylori

) infection and its effect on the proliferation of CD3

T cells.

Methods

H.pylori

infection model was established in C57 mice and gastric epithelial cell lines (AGS and HGC-27). RT-PCR was used to detect the expression of ADM in the cells incubated with or without

H.pylori

. The protein levels of ADM

AKT and p-AKT were detected by Western blotting. The function of ADM on the proliferation of CD3

T cells were evaluated by flow cytometry.

Results

H.pylori

infection increased the expression of ADM in AGS cells and HGC-27 cells in a time-and dose-dependent manner (

0.05). While

blocking PI3K/AKT signal pathway reversed above increase induced by

H.pylori

(

0.05). Compared with uninfected mice

the infection also enhanced the ADM expression in the mouse gastric tissue. Compared with WT

H.pylori

infected AGS and HGC-27 cells

knockout of cagA resulted in decrease in ADM expression which was induced by

H.pylori

infection (

0.05). ADM induced the proliferation of CD3

T cells in a dose-dependent manner

and enhanced the expression of IFN-γ (

0.05).

Conclusion

H.pylori

infection up-regulates ADM expression through PI3K/AKT signaling pathway activation

and ADM exerts immunoregulatory effect by promoting proliferation and IFN-γ production of CD3

T cells.

关键词

Keywords

references

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⁰

Role and mechanism of adrenomedullin expression in Helicobacter pylori infection

DOI：10.16016/j.1000-5404.201709041

摘要

Abstract

关键词

Keywords

references