Deltex-1 overexpression suppresses proliferation and promotes differentiation of bone marrow mesenchymal stem cells into smooth muscle cells <italic style="font-style: italic">in vitro</italic>

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Deltex-1 overexpression suppresses proliferation and promotes differentiation of bone marrow mesenchymal stem cells into smooth muscle cells in vitro

Basic Medicine | 更新时间：2026-01-09

- Deltex-1 overexpression suppresses proliferation and promotes differentiation of bone marrow mesenchymal stem cells into smooth muscle cells in vitro
- Journal of Third Military Medical University Vol. 40, Issue 2, Pages: 109-115(2018)
- 作者机构：
  
  1.400021 重庆，重庆市妇幼保健院
  2.400038 重庆，陆军军医大学(第三军医大学)第一附属医院妇产科
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(30772309)
- DOI：10.16016/j.1000-5404.201708201
  CLC： R322.74;R329.24;R394.2
- Received：30 August 2017，
  
  Revised：2017-10-17，
  
  Published：30 January 2018
- 稿件说明：
移动端阅览
Ying WANG, Yanzhou WANG, Lubin LIU, et al. Deltex-1 overexpression suppresses proliferation and promotes differentiation of bone marrow mesenchymal stem cells into smooth muscle cells in vitro[J]. Journal of Third Military Medical University, 2018, 40(2): 109-115.
DOI：

Ying WANG, Yanzhou WANG, Lubin LIU, et al. Deltex-1 overexpression suppresses proliferation and promotes differentiation of bone marrow mesenchymal stem cells into smooth muscle cells in vitro[J]. Journal of Third Military Medical University, 2018, 40(2): 109-115. DOI： 10.16016/j.1000-5404.201708201.

摘要

目的

2

探讨Deltex-1对骨髓间充质干细胞(bone marrow mesenchymal stem cells

bMSCs)增殖及其向平滑肌细胞(smooth muscle cells，SMCs)分化的影响。

方法

2

分离培养大鼠骨髓bMSCs，采用流式细胞仪检测鉴定。通过Deltex-1过表达腺病毒载体pAd/Deltex-1感染bMSCs后，采用CCK-8(cell count kit-8)法检测Deltex-1过表达对bMSCs增殖的影响; 将未感染、经Deltex-1病毒感染及经空病毒感染的bMSCs与SMCs共培养，采用免疫荧光细胞化学、RT-PCR及Western blot分别检测其bMSCs中SMCs标志物平滑肌肌球蛋白重链(smooth muscle myosin heavy chain，SM-MHC)及Notch信号通路相关因子Notch-1的表达，单纯培养的bMSCs、SMCs的相同检测作正常对照。

结果

2

成功分离、培养大鼠bMSCs。CCK-8检测证实：与对照比较，经Deltex-1病毒感染的bMSCs生长减慢，48 h开始显得更为明显(

P

<

0.05，

P

<

0.01);免疫荧光细胞化学、RT-PCR及Western blot检测显示：与对照比较，经Deltex-1病毒感染的bMSCs与SMCs共培养后显著表达SMCs的标志物SM-MHC，较弱表达Notch信号通路相关因子Notch-1(

P

<

0.01)。

结论

2

Deltex-1过表达可抑制bMSCs的增殖，并促进其向SMCs分化。

Abstract

Objective

2

To investigate the effect of Deltex-1 overexpression on the proliferation of bone marrow mesenchymal stem cells (bMSCs) and their differentiation into smooth muscle cells (SMCs) in vitro.

Methods

2

Rat bMSCs were isolated

cultured and identified. The effect of Deltex-1 overexpression on the proliferation of bMSCs infected with the adenovirus vector pAd/Deltex-1 was observed using cell count kit-8 (CCK-8). In a co-culture system with SMCs

bMSCs infected with pAd/Deltex-1 or an empty viral vector or with no treatment were examined for the expression of smooth muscle myosin heavy chain (SM-MHC

a marker of SMCs) using immunofluorescence cytochemistry staining

RT-PCR and Western blotting

with bMSCs and SMCs without treatment in separate cultures as normal controls.

Results

2

Compared with the control cells

bMSCs infected by the adenovirus vector pAd/Deltex-1 exhibited significantly lowered growth rate

which was especially obvious at 48 h after the infection (

P

<

0.05

0.01). The

Results

2

of immunofluorescence cytochemistry

real-time PCR and Western blotting all showed that bMSCs infected with the Deltex-1 vector and co-cultured with SMCs expressed a significantly increased level of SM-MHC with a lowed expression of Notch-1 (

P

<

0.01).

Conclusion

2

Deltex-1 overexpression causes suppression of bMSC proliferation and promotes the differentiation of bMSCs into smooth muscle cells in vitro.

关键词

Keywords

references

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