Advanced glycation end products enhance proliferation and invasion of human colon cancer SW480 cells through JAK2/STAT3 pathway

Wuyi WANG; Yong CHENG; Lin WAN

doi:10.16016/j.1000-5404.201708199

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Advanced glycation end products enhance proliferation and invasion of human colon cancer SW480 cells through JAK2/STAT3 pathway

Basic Medicine | 更新时间：2026-01-09

- Advanced glycation end products enhance proliferation and invasion of human colon cancer SW480 cells through JAK2/STAT3 pathway
- Journal of Third Military Medical University Vol. 39, Issue 24, Pages: 2390-2395(2017)
- 作者机构：
  
  400016 重庆, 重庆医科大学附属第一医院胃肠外科
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201708199
  CLC： R394.2; R730.23; R735.35
- Received：31 August 2017，
  
  Revised：2017-10-08，
  
  Published：30 December 2017
- 稿件说明：
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Wuyi WANG, Yong CHENG, Lin WAN. Advanced glycation end products enhance proliferation and invasion of human colon cancer SW480 cells through JAK2/STAT3 pathway[J]. Journal of Third Military Medical University, 2017, 39(24): 2390-2395.
DOI：

Wuyi WANG, Yong CHENG, Lin WAN. Advanced glycation end products enhance proliferation and invasion of human colon cancer SW480 cells through JAK2/STAT3 pathway[J]. Journal of Third Military Medical University, 2017, 39(24): 2390-2395. DOI： 10.16016/j.1000-5404.201708199.

摘要

目的

观察糖基化终末产物(advanced glycation end products

AGEs)对结肠癌SW480细胞增殖、凋亡、侵袭、迁移及上皮间质转化的影响，并探讨其相关机制。

方法

将结肠癌SW480细胞分为对照组、AGEs组(200 μg/mL AGEs)、AGEs(200 μg/mL)+AG490(50 μmol/L)组，CCK-8检测细胞增殖活力，流式细胞术检测细胞周期、凋亡，Transwell实验检测细胞体外侵袭能力，划痕实验检测细胞体外迁移能力。不同浓度(0、50、100、200 μg/mL)AGEs和200 μg/mL AGEs、50 μmol/L AG490单独处理及二者联合处理SW480细胞后，Western blot检测上皮间质转化相关分子标志物E-cadherin、N-cadherin、Vimentin及JAK2/STAT3信号通路相关分子的变化。

结果

与对照组比较，AGEs能明显促进结肠癌SW480细胞的增殖，减少G

/S期阻滞，抑制凋亡，增强体外的侵袭、迁移能力(

＜0.05)。与AGEs组比较，AGEs+AG490组结肠癌SW480细胞增殖减少，G

/S期阻滞增加，凋亡增加，侵袭、迁移能力降低，差异有统计学意义(

＜0.05)。Western blot检测结果显示，AGEs处理结肠癌SW480细胞后E-cadherin表达降低，N-cadherin、Vimentin、p-STAT3、p-JAK2表达升高，而AG490可逆转AGEs对结肠癌SW480细胞的作用。

结论

AGEs能够通过JAK2/STAT3信号通路促进结肠癌SW480细胞的增殖，抑制凋亡，增强侵袭、迁移能力，促进上皮间质转化。

Abstract

Objective

To explore the effects and the possible mechanisms of advanced glycation products (AGEs) on the proliferation

invasion and epithelial-mesenchymal transition(EMT) of human colon cancer cell line SW480.

Methods

The SW480 cells were divided into control group

AGEs (200 μg/mL) group

and AGEs (200 μg/mL)+AG490(50 μmol/L) group. After the treatment

the cell proliferation

cell cycle and apoptosis

invasion and migration were tested by CCK-8 assay

flow cytometry

Transwell chamber test and wound healing assay. After the cells were treated with 0

100 and 200 μg/mL AGEs or 200 μg/mL AGEs plus 50 μmol/L AG490

the expression levels of EMT markers

E-cadherin

N-cadherin and Vimentin

and JAK2/STAT3 pathway molecules

STAT3

p-STAT3

JAK2 and p-JAK2 were detected by Western blotting.

Results

Compared with the control group

AGEs significantly promoted the proliferation

invasion and migration of SW480 cells

reduced the number of cells arrested at G

/S phase

and inhibited cell apoptosis (

0.05). Whereas

these effects were obviously reversed by the addition of 50 μmol/L AG490 (

0.05). Western blotting showed that AGEs increased the expression levels of N-cadherin

Vimentin

p-STAT3 and p-JAK2

and decreased that of E-cadherin. But AG490 reversed such changes resulted from AGEs treatment.

Conclusion

AGEs enhance proliferation

invasion and migration

and promote EMT in human colon cancer cell line SW480 through JAK2/STAT3 signaling pathway.

关键词

Keywords

references

TORRE L A, BRAY F, SIEGEL R L, et al. Global cancer statistics, 2012[J]. CA Cancer J Clin, 2015, 65(2): 87-108. DOI: 10.3322/caac.21262.

陈万青, 郑荣寿, 张思维, 等. 2012年中国恶性肿瘤发病和死亡分析[J].中国肿瘤, 2016, 25(1): 1-8. DOI: 10.11735/j.issn.1004-0242.2016.01.A001.

CHEN W Q, ZHENG R S, ZAHNG S W, et al. Report of cancer incidence and mortality in china, 2012[J]. China Cancer, 2016, 25(1): 1-8. DOI: 10.11735/j.issn.1004-0242.2016.01.A001.

YAMAGISHI S, NAKAMURA N, SUEMATSU M, et al. Advanced glycation end products: amolecular target for vascular complications in diabetes[J]. Mol Med, 2015, 21 Suppl 1: S32-S40.

WU C H, WU T Y, LI C C, et al. Impact of diabetes mellitus on the prognosis of patients with oral squamous cell carcinoma: a retrospective cohort study[J]. Ann Surg Oncol, 2010, 17(8): 2175-2183. DOI: 10.1245/s10434-010-0996-1.

NASS N, IGNATOV A, ANDREAS L, et al. Accumulation of the advanced glycation end product carboxymethyl lysine in breast cancer is positively associated with estrogen receptor expression and unfavorable prognosis in estrogen receptor-negative cases[J]. Histochem Cell Biol, 2017, 147(5): 625-634. DOI: 10.1007/s00418-016-1534-4.

邱俊霖, 钟宇华, 梁华晟, 等.糖尿病与结肠癌关系的研究进展[J].医学综述, 2015, 21(10): 1783-1785. DOI: 10.3969/j.issn.1006-2084.2015.10.020.

QIU J L, ZHONG Y H, LIANG H S, et al. Progress in the study on the correlation between diabetes mellitus and colon cancer[J]. Med Recapitulate, 2015, 21(10): 1783-1785. DOI: 10.3969/j.issn.1006-2084.2015.10.020.

JEON J Y, JEONG D H, PARK M G, et al. Impact of diabetes on oncologic outcome of colorectal cancer patients: colon vs . rectal cancer[J ] . PLoS ONE, 2013, 8(2): e55196. DOI: 10.1371/journal.pone.0055196.

SINGH R, BARDEN A, MORI T, et al. Advanced glycation end-products: a review[J]. Diabetologia, 2001, 44(2): 129-146. DOI: 10.1007/s001250051591.

LOPEZ-DIEZ R, RASTROJO A, VILLATE O, et al. Complex tissue-specific patterns and distribution of multiple RAGE splice variants in different mammals[J]. Genome Biol Evol, 2013, 5(12): 2420-2435. DOI: 10.1007/s001250051591.

CAO H, XU E, LIU H, et al. Epithelial-mesenchymal transition in colorectal cancer metastasis: A system review[J]. Pathol Res Pract, 2015, 211(8): 557-569. DOI: 10.1016/j.prp.2015.05.010.

KALLURI R, WEINBERG R A. The basics of epithelial-mesenchymal transition[J]. J Clin Invest, 2009, 119(6): 1420-1428. DOI: 10.1172/JCI39104.

KAY A M, SIMPSON C L, STEWART J A. The Role of AGE/RAGE Signaling in Diabetes-Mediated Vascular Calcification[J]. Journal of Diabetes Research, 2016, 2016: 1-8. DOI: 10.1155/2016/6809703.

BAO J M, HE M Y, LIU Y W, et al. AGE/RAGE/Akt pathway contributes to prostate cancer cell proliferation by promoting Rb phosphorylation and degradation[J]. Am J Cancer Res, 2015, 5(5): 1741-1750. DOI: 10.21037/tau.2016.s044.

陈海云, 张楠, 从丽, 等.胃癌中p-Stat3活化和SOCS3表达的临床病理意义及其对预后的影响[J].重庆医学, 2014, 43(32): 4316-4319, 4330. DOI: 10.3969/j.issn.1671-8348.2014.32.014.

CHEN H Y, ZHANG N, CONG Li, et al. Clinical pathological and prognostic significance of activated p-stat3 and expressed socs3 in patients with gastric cancer[J]. Chongqing Med, 2014, 43(32): 4316-4319, 4330. DOI: 10.3969/j.issn.1671-8348.2014.32.014.

乔丽, 李新技, 杨志勇, 等. STAT3及p-STAT3在皮肤鳞状细胞癌中的表达及其与鳞癌临床病理特征的关系[J].山西医科大学学报, 2015, 46(12): 1207-1210.

QIAO L, LI X J, YANG Z Y, et al. Expression of stat3 and p-stat3 in human squamous carcinoma cells and its relationship with clinicopathological charac-teristics[J]. J Shanxi Med Univ, 2015, 46(12): 1207-1210. DOI: 10.13753/j.issn.1007-6611.2015.12.011.

李容, 王新文, 杨晓辉, 等.细胞命运之终点——细胞死亡[J].科学通报, 2016, 61(18): 1983-1987.

LI R, WANG X W, YANG X H, et al. The end of cell fate—cell death[J]. Chin Sci Bull, 2016, 61(18): 1983-1987.

WANG Y, WU Z, HU L. Epithelial-Mesenchymal Transition Phenotype, Metformin, and Survival for Colorectal Cancer Patients with Diabetes Mellitus Ⅱ[J]. Gastroenterol Res Pract, 2017, 2017(18): 2520581. DOI: 10.1155/2017/2520581.

ZUBAIR H, BHARDWAJ A, AHMAD A, et al. Hydroxytyrosol Induces Apoptosis and Cell Cycle Arrest and Suppresses Multiple Oncogenic Signaling Pathways in Prostate Cancer Cells[J]. Nutr Cancer, 2017: 1.

伍鑫, 程勇, 庞云, 等.终末糖基化产物对结肠癌SW620细胞EMT及肿瘤干细胞标志物的影响[J].中国肿瘤生物治疗杂志, 2016, 23(3): 355-359. DOI: 10.3872/j.issn.1007-385X.2016.03.010.

WU X, CHENG Y, PANG Y, et al. Effects of advanced glycation end products on epithelial-mesenchymal transition and cancer stem cell associated markers in human colon cancer cell line sw620[J]. Chin J Cancer Biotherap, 2016, 23(3): 355-359. DOI: 10.3872/j.issn.1007-385X.2016.03.010.

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