Construction of aptamer electrochemical sensor based on SWCNHs@HPtCs complexes for perforin detection

Fanfei MENG; Fei LIU; Chang LIU; Dongneng JIANG; Xiaoyun PU

doi:10.16016/j.1000-5404.201708196

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Construction of aptamer electrochemical sensor based on SWCNHs@HPtCs complexes for perforin detection

Basic Medicine | 更新时间：2026-01-09

- Construction of aptamer electrochemical sensor based on SWCNHs@HPtCs complexes for perforin detection
- Journal of Third Military Medical University Vol. 40, Issue 1, Pages: 76-81(2018)
- 作者机构：
  
  400037 重庆, 陆军军医大学新桥医院检验科
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201708196
  CLC： R313;R341;R392-33
- Received：29 August 2017，
  
  Revised：2017-10-30，
  
  Published：15 January 2018
- 稿件说明：
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Fanfei MENG, Fei LIU, Chang LIU, et al. Construction of aptamer electrochemical sensor based on SWCNHs@HPtCs complexes for perforin detection[J]. Journal of Third Military Medical University, 2018, 40(1): 76-81.
DOI：

Fanfei MENG, Fei LIU, Chang LIU, et al. Construction of aptamer electrochemical sensor based on SWCNHs@HPtCs complexes for perforin detection[J]. Journal of Third Military Medical University, 2018, 40(1): 76-81. DOI： 10.16016/j.1000-5404.201708196.

摘要

目的

构建一种基于单壁碳纳米角(single-walled carbon nanohorns

SWCNHs)与空心纳米铂链(hollow Pt chains

HPtCs)复合物(SWCNHs@HPtCs)的电化学适配体传感器。

方法

将单壁碳纳米管(single-wall carbon nanotubes

SWCNTs)溶液滴加在洁净的玻碳电极上(glassy carbon electrode

GCE)

使之在电极表面形成一层膜

通过循环伏安法(cyclic voltammetry

CV)在电极表面电沉积纳米金(Au nanoparticles

AuNPs)

得到AuNPs/ SWCNTs/GCE。进一步通过金硫键将穿孔素适配体固载在电极表面。制备SWCNHs@HPtCs复合物

固载穿孔素抗体和氨基二茂铁(aminoferrocene

AFc)

当目标物穿孔素存在时

可与适配体和抗体形成夹心结构

使标记有AFc的SWCNHs@HPtCs复合物连接到电极表面

通过示差脉冲伏安法(differential pulse voltammetry

DPV)检测AFc还原峰电流值变化

实现对穿孔素的定量检测。

结果

成功制备了SWCNHs@HPtCs复合物。反应底液的最佳pH值为6.5

复合物最佳孵育时间为60 min。在优化实验条件下

连续扫描50圈

峰电流值下降6.3%。穿孔素浓度在1~20 nmol/L范围内与DPV峰电流值呈良好的线性关系

检出限为0.56 nmol/L。

结论

该电化学传感器具有电流响应快

灵敏度高

稳定性好

选择性好的特点

可应用于穿孔素的检测。

Abstract

Objective

To construct an aptamer electrochemical sensor based on single-walled carbon nanohorns (SWCNHs) and hollow Pt chains (HPtCs) complex (SWCNHs@HPtCs).

Methods

A solution containing single-wall carbon nanotubes (SWCNTs) was added on a clean glassy carbon electrode (GCE) to form a film on the surface of the electrode. A film of nano-gold was electrodeposited on the surface of the electrode by cyclic voltammetry (CV) to form AuNPs/SWCNTs/GCE. The SWCNHs@HPtCs complex was prepared by immobilizing perforin aptamers on the electrode surface and labeling aminoferrocene (AFc) as a signal carrier. When the target existed

the aptamers

perforin and antibodies formed a sandwich structure

which would connect AFc labeled HPtCs@SWCNHs complex to the electrode surface. And then detection of differential pulse voltammetry (DPV) would display the changes of AFc reduction peak current to achieve the identification of perforin and quantitative detection.

Results

The SWCNHs@HPtCs complex was prepared successfully. The optimal pH value of the detection solution was 6.5 and the optimal incubation time of the complex was 60 min. Under the optimal experimental conditions

the current was decreased by 6.3% after 50 cycles of consecutive scanning. There was a good linear relationship between the concentration of perforin in the range of 1 to 20 nmol/L and the peak current of the DPV. The detection limit was 0.56 nmol/L.

Conclusion

Our prepared electrochemical sensor has the characteristics of fast current response

high sensitivity

good stability and good selectivity

and can be applied to the detection of perforin.

关键词

Keywords

references

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