Alkaline phosphatase, liver/bone/kidney, inhibits proliferation of ovarian cancer cell line SKOV3

Lijuan CHENG; Shijie ZHAN; Bin LIU; Hua LINGHU

doi:10.16016/j.1000-5404.201708130

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Alkaline phosphatase, liver/bone/kidney, inhibits proliferation of ovarian cancer cell line SKOV3

Basic Medicine | 更新时间：2026-01-09

- Alkaline phosphatase, liver/bone/kidney, inhibits proliferation of ovarian cancer cell line SKOV3
- Journal of Third Military Medical University Vol. 40, Issue 1, Pages: 58-63(2018)
- 作者机构：
  
  1.400016 重庆, 重庆医科大学:附属第一医院妇产科
  2.400016 重庆, 重庆医科大学:基础医学院病理科
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201708130
  CLC： R394.2;R730.23;R737.31
- Received：21 August 2017，
  
  Revised：2017-10-19，
  
  Published：15 January 2018
- 稿件说明：
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Lijuan CHENG, Shijie ZHAN, Bin LIU, et al. Alkaline phosphatase, liver/bone/kidney, inhibits proliferation of ovarian cancer cell line SKOV3[J]. Journal of Third Military Medical University, 2018, 40(1): 58-63.
DOI：

Lijuan CHENG, Shijie ZHAN, Bin LIU, et al. Alkaline phosphatase, liver/bone/kidney, inhibits proliferation of ovarian cancer cell line SKOV3[J]. Journal of Third Military Medical University, 2018, 40(1): 58-63. DOI： 10.16016/j.1000-5404.201708130.

摘要

目的

研究肝/肾/骨型碱性磷酸酶基因(alkaline phosphatase

liver/bone/kidney

ALPL)在卵巢癌细胞株中的表达及其对卵巢癌细胞SKOV3增殖的影响。

方法

通过qRT-PCR检测ALPL在不同卵巢癌细胞株(HEY、SKOV3、8910、ES-2、OVCAR3、3AO、A2780) mRNA中的表达

并与正常卵巢组织进行比较。在低表达ALPL的卵巢癌细胞株SKOV3中外源性过表达ALPL

通过MTT法、平板克隆实验检测细胞的增殖

流式细胞仪检测细胞周期。通过建立裸鼠皮下种植瘤模型

观察过表达ALPL的SKOV3细胞在体内的成瘤能力。

结果

ALPL在卵巢癌细胞中的mRNA表达低于正常卵巢组织(

0.01)

外源性过表达ALPL可抑制SKOV3细胞的增殖和克隆形成率(

0.01)

阻滞细胞G

期向S的转化

并抑制肿瘤细胞的成瘤能力(

0.05)。

结论

ALPL表达的恢复可以明显抑制卵巢癌细胞SKOV3的增殖及成瘤能力。

Abstract

Objective

To investigate the expression of tissue-nonspecific alkaline phosphatase

liver/bone/kidney (ALPL) in human ovarian cancer cell lines

and determine its effect on the proliferation of ovarian cancer SKOV3 cells.

Methods

qRT-PCR was used to detect the expression of ALPL mRNA level in 7 ovarian cancer cell lines (HEY

SKOV3

8910

ES-2

OVCAR3

3AO and A2780). The results were compared with its level in normal ovarian tissues. After the SKOV3 cells (with low expression of ALPL) were interfered with lentiviral vector to induce ALPL overexpression

cell proliferation was measured by MTT assay and plate colony formation assay

and cell cycle were assessed by flow cytometry. Tumorigenesis was evaluated through xenograft growth of ALPL-overexpressed SKOV3 cells in nude mice.

Results

The expression level of ALPL was significantly lower in all the ovarian cancer cell lines studied when compared with that in normal ovarian tissues (

0.01). ALPL Over-expression resulted in the decreases in cell proliferation and clone formation rate (

0.01) and blocked the transition of G

phase to S phase in SKOV3 cells

as well as suppressed the subcutaneous tumorigenesis in xenografts tumor model (

0.05).

Conclusion

Reversion of ALPL expression level might obviously suppress the cell proliferation and tumorigenesis of SKOV3 cells.

关键词

Keywords

references

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